Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I0 R1	2892	75.3	5698161	66.1%	3766484	59.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,595,383	T→G	27.9%	T405T (ACA→ACC)	arsB ←	arsenite/antimonite transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,595,383	0	T	G	27.9%	145.5 / 97.9	122	T405T (ACA→ACC)	arsB	arsenite/antimonite transporter
Reads supporting (aligned to +/- strand): ref base T (46/42); new base G (18/16); total (64/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

AGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAG  >  minE/2595314‑2595453

aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                                       <  1:4669639/71‑1 (MQ=255)

aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                                       <  1:4786190/71‑1 (MQ=255)

aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                                       <  1:3202795/71‑1 (MQ=255)

aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                                       <  1:1826622/71‑1 (MQ=255)

 gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                                       >  1:1153276/1‑70 (MQ=255)

 gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                                       >  1:5256164/1‑70 (MQ=255)

         gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg                                                               >  1:825321/1‑70 (MQ=255)

         gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg                                                               >  1:1827920/1‑70 (MQ=255)

         gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt                                                              <  1:5453997/71‑1 (MQ=255)

         gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt                                                              <  1:1696360/71‑1 (MQ=255)

         gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt                                                              <  1:163546/71‑1 (MQ=255)

          agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa                                                             <  1:5410098/71‑1 (MQ=255)

          agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa                                                             <  1:1321587/71‑1 (MQ=255)

                cacgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcc                                                       >  1:5377867/3‑71 (MQ=255)

                 gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc                                                      >  1:5127962/1‑71 (MQ=255)

                 gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc                                                      >  1:5591103/1‑71 (MQ=255)

                 acgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATccc                                                      >  1:3025161/2‑71 (MQ=255)

                 acgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATccc                                                      >  1:2590656/2‑71 (MQ=255)

                  cgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc                                                     <  1:3471919/71‑1 (MQ=255)

                    ccAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAg                                                   >  1:4473558/1‑71 (MQ=255)

                      aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt                                                 <  1:130726/71‑1 (MQ=255)

                       gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc                                                     <  1:4704743/66‑1 (MQ=255)

                        cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                                               <  1:513441/71‑1 (MQ=255)

                        cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                                               <  1:29933/71‑1 (MQ=255)

                          cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                                               >  1:5070701/1‑69 (MQ=255)

                          cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                                               >  1:3649646/1‑69 (MQ=255)

                           ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg                                            >  1:3870623/2‑69 (MQ=255)

                              cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGCTAGTCa                                         <  1:5555/71‑1 (MQ=255)

                              cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                                         <  1:214073/71‑1 (MQ=255)

                                gcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                                       <  1:4893225/71‑1 (MQ=255)

                                 cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                                      <  1:614030/71‑1 (MQ=255)

                                 cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                                      <  1:1256084/71‑1 (MQ=255)

                                 cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                                      <  1:4148128/71‑1 (MQ=255)

                                  gTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                                      <  1:2985680/70‑1 (MQ=255)

                                   tgacaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTaa                                                            >  1:60203/3‑47 (MQ=16)

                                   tgacaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc                                                     <  1:4123704/52‑1 (MQ=255)

                                   tgacaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttt                                    <  1:1203956/69‑2 (MQ=255)

                                   tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                                               <  1:1595552/60‑1 (MQ=255)

                                   tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc                                    <  1:1791542/71‑1 (MQ=255)

                                    cacaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc                                          >  1:5575767/1‑64 (MQ=255)

                                     acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt                                  <  1:3852905/71‑1 (MQ=255)

                                     acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttt                                   <  1:2152288/70‑3 (MQ=255)

                                     acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa                                                                >  1:2766781/1‑41 (MQ=255)

                                     acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa                                                                >  1:1231302/1‑41 (MQ=255)

                                     acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc                                                       >  1:24804/1‑50 (MQ=255)

                                     acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAg                                                   >  1:2919375/1‑54 (MQ=255)

                                     acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                                  >  1:4167808/1‑71 (MQ=255)

                                     acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                                  >  1:4352398/1‑71 (MQ=255)

                                     acaAACAGCACAGGCAGGGTCATGATAATCCCTGAACGGAAGTAATATcc                                                       >  1:5395728/1‑50 (MQ=255)

                                     acaAACAGCAAAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                                  >  1:2334813/1‑71 (MQ=255)

                                      caAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttttc                                 <  1:3068514/71‑1 (MQ=255)

                                      caAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc                                 >  1:2882560/1‑71 (MQ=255)

                                       aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct                                >  1:4119604/1‑71 (MQ=255)

                                       aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct                                >  1:1942030/1‑71 (MQ=255)

                                       aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct                                >  1:760969/1‑71 (MQ=255)

                                        aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc                                                       >  1:4917045/1‑47 (MQ=255)

                                        aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTg                               >  1:1783083/1‑71 (MQ=255)

                                           aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg                             >  1:4332141/1‑68 (MQ=255)

                                              acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATtcttct                                >  1:34713/1‑64 (MQ=255)

                                                aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt                                           <  1:2549641/51‑1 (MQ=255)

                                                aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa                       >  1:1731795/1‑71 (MQ=255)

                                                 ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc                      <  1:1130225/71‑1 (MQ=255)

                                                   caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt                    <  1:4120927/71‑1 (MQ=255)

                                                   caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt                    <  1:1486229/71‑1 (MQ=255)

                                                    aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                                       >  1:2803758/1‑51 (MQ=255)

                                                    aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                                       >  1:3604778/1‑51 (MQ=255)

                                                    acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                   >  1:1389504/4‑71 (MQ=255)

                                                    acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                   <  1:555069/68‑1 (MQ=255)

                                                     gggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGc                  <  1:1524422/71‑1 (MQ=255)

                                                     cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCa                                         <  1:3979752/46‑1 (MQ=16)

                                                     cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                   <  1:2483560/68‑1 (MQ=255)

                                                     cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc                  <  1:52761/69‑1 (MQ=255)

                                                     cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc                  <  1:725182/69‑1 (MQ=255)

                                                      ggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc                                    <  1:3415756/52‑1 (MQ=255)

                                                        tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGaa                           <  1:5615276/59‑1 (MQ=255)

                                                        tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc               >  1:4912920/1‑71 (MQ=255)

                                                        tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg                         >  1:4417615/1‑61 (MQ=255)

                                                        tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg                         >  1:774505/1‑61 (MQ=255)

                                                         cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtt                                     >  1:5272059/1‑48 (MQ=19)

                                                          aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc                                          >  1:431553/1‑42 (MQ=255)

                                                          aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa             <  1:5321996/71‑1 (MQ=255)

                                                          aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                   >  1:833614/1‑65 (MQ=255)

                                                          aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa             <  1:203005/71‑1 (MQ=255)

                                                           tGATAATCCCTGTACGGAAGTAATATCCCCAGCTg                                                <  1:3026791/35‑1 (MQ=255)

                                                           tGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                                       <  1:4453964/44‑1 (MQ=255)

                                                             aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                                         <  1:4261952/40‑1 (MQ=255)

                                                             aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                                  >  1:4746272/1‑47 (MQ=255)

                                                             aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                                  >  1:2099194/1‑47 (MQ=255)

                                                             aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag          <  1:5564881/71‑1 (MQ=255)

                                                              tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt                                           >  1:700295/1‑37 (MQ=255)

                                                              tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                         >  1:470386/1‑55 (MQ=255)

                                                              tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                         >  1:4243826/1‑55 (MQ=255)

                                                               aaTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTg                               >  1:2184508/1‑48 (MQ=19)

                                                                 tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc      >  1:4444805/1‑71 (MQ=255)

                                                                  cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta        >  1:5274802/1‑68 (MQ=255)

                                                                  cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta        >  1:516239/1‑68 (MQ=255)

                                                                  cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta        >  1:2801405/1‑68 (MQ=255)

                                                                   ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg     >  1:4551306/1‑70 (MQ=255)

                                                                     tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag  >  1:3436204/1‑71 (MQ=255)

                                                                     tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag  >  1:549829/1‑71 (MQ=255)

AGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAG  >  minE/2595314‑2595453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A