Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,595,405 |
C→G |
28.7% |
S398T (AGC→ACC) ‡ |
arsB ← |
arsenite/antimonite transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,595,405 | 0 | C | G | 28.7%
| 208.4
/ 100.8
| 139 | S398T (AGC→ACC) ‡ | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base C (52/47); new base G (18/22); total (70/69) |
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 139 total aligned reads displayed.
AGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAA > minE/2595336‑2595474
|
aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt < 1:130726/71‑1 (MQ=255)
gcgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt > 1:2839713/1‑70 (MQ=255)
cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:513441/71‑1 (MQ=255)
cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:1243161/71‑1 (MQ=255)
cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa > 1:5070701/1‑69 (MQ=255)
ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg > 1:3870623/2‑69 (MQ=255)
cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa < 1:214073/71‑1 (MQ=255)
agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa > 1:5646237/1‑70 (MQ=255)
agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa < 1:1674147/70‑1 (MQ=255)
gcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata < 1:4893225/71‑1 (MQ=255)
cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat < 1:1256084/71‑1 (MQ=255)
cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat < 1:614030/71‑1 (MQ=255)
cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat < 1:4148128/71‑1 (MQ=255)
gTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat < 1:2985680/70‑1 (MQ=255)
tgacaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttt < 1:1203956/69‑2 (MQ=255)
tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:4270512/60‑1 (MQ=255)
tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc < 1:1791542/71‑1 (MQ=255)
acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt < 1:3852905/71‑1 (MQ=255)
acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt > 1:4352398/1‑71 (MQ=255)
caAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttttc < 1:3068514/71‑1 (MQ=255)
caAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc > 1:2882560/1‑71 (MQ=255)
aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct > 1:760969/1‑71 (MQ=255)
aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct > 1:1942030/1‑71 (MQ=255)
aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTg > 1:1783083/1‑71 (MQ=255)
cagcagAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGc > 1:5555800/1‑70 (MQ=255)
aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg > 1:4332141/1‑68 (MQ=255)
aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg > 1:74608/1‑68 (MQ=255)
aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt < 1:2549641/51‑1 (MQ=255)
aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa > 1:1731795/1‑71 (MQ=255)
ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc < 1:1130225/71‑1 (MQ=255)
gcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg < 1:5252685/71‑1 (MQ=255)
caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt < 1:4120927/71‑1 (MQ=255)
aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata > 1:2803758/1‑51 (MQ=255)
aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata > 1:3604778/1‑51 (MQ=255)
acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg > 1:1389504/4‑71 (MQ=255)
acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:555069/68‑1 (MQ=255)
acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:5285263/68‑1 (MQ=255)
gggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGc < 1:1524422/71‑1 (MQ=255)
cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCa < 1:3979752/46‑1 (MQ=16)
cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:2483560/68‑1 (MQ=255)
tgTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc < 1:2257996/65‑1 (MQ=255)
ggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc < 1:3415756/52‑1 (MQ=255)
tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGaa < 1:5615276/59‑1 (MQ=255)
tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc > 1:4912920/1‑71 (MQ=255)
tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg > 1:774505/1‑61 (MQ=255)
tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg > 1:4417615/1‑61 (MQ=255)
cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtt > 1:5272059/1‑48 (MQ=19)
cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGc > 1:3535977/1‑70 (MQ=255)
aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc > 1:4134779/1‑42 (MQ=255)
aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc > 1:431553/1‑42 (MQ=255)
aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa < 1:5321996/71‑1 (MQ=255)
aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa < 1:203005/71‑1 (MQ=255)
tGATAATCCCTGTACGGAAGTAATATCCCCAGCTg < 1:3026791/35‑1 (MQ=255)
tGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata < 1:3012696/44‑1 (MQ=255)
tGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata < 1:4453964/44‑1 (MQ=255)
aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt > 1:2099194/1‑47 (MQ=255)
aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt > 1:4746272/1‑47 (MQ=255)
aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag < 1:5564881/71‑1 (MQ=255)
tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt > 1:700295/1‑37 (MQ=255)
tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg > 1:4243826/1‑55 (MQ=255)
aaTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTg > 1:2184508/1‑48 (MQ=19)
tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc > 1:4444805/1‑71 (MQ=255)
cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta > 1:5274802/1‑68 (MQ=255)
cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta > 1:2801405/1‑68 (MQ=255)
cggTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagtt < 1:5530447/69‑2 (MQ=255)
tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag > 1:3436204/1‑71 (MQ=255)
tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag > 1:549829/1‑71 (MQ=255)
gTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc > 1:4700516/1‑71 (MQ=255)
tACGGAAGTAATATCCCCAGCTGATAGTGATATTCTTCTGCGAAAGTACGTGCAGCCa < 1:5601061/58‑1 (MQ=255)
tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa > 1:1377180/1‑48 (MQ=255)
tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa > 1:4353467/1‑48 (MQ=255)
tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa < 1:1988895/58‑1 (MQ=255)
tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag < 1:846704/69‑1 (MQ=255)
accgAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc < 1:4689893/67‑1 (MQ=255)
aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc > 1:4250600/1‑69 (MQ=255)
cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg > 1:1359458/1‑71 (MQ=255)
ggAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg > 1:2068162/1‑71 (MQ=255)
aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc > 1:4607899/1‑70 (MQ=255)
aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc > 1:1061447/1‑70 (MQ=255)
aaGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCac < 1:4735523/54‑2 (MQ=255)
aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtg > 1:2813062/1‑70 (MQ=255)
aGTAATATCCCCAAGTGATTGTCATATTTTTCAGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt < 1:878483/70‑1 (MQ=255)
gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:457075/45‑1 (MQ=255)
tAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc > 1:3296041/1‑68 (MQ=255)
aaTATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCa > 1:3256388/1‑45 (MQ=255)
atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg > 1:292915/1‑64 (MQ=255)
atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa < 1:5022952/70‑1 (MQ=255)
tatCCCCAGCTGATAGTCATATTCTTCTGCGaaat < 1:367818/35‑2 (MQ=255)
tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAg < 1:4743152/35‑1 (MQ=255)
tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt < 1:2615888/71‑1 (MQ=255)
atCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAtt > 1:2975719/1‑71 (MQ=255)
tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg > 1:3338200/1‑71 (MQ=255)
ccccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTgg > 1:4586071/1‑71 (MQ=255)
cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg > 1:4068552/1‑71 (MQ=255)
cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg > 1:1531737/1‑71 (MQ=255)
cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga < 1:1476712/43‑1 (MQ=255)
cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga < 1:262241/43‑1 (MQ=255)
cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga < 1:1632740/43‑1 (MQ=255)
aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt > 1:2497030/1‑70 (MQ=255)
gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc > 1:1134345/1‑46 (MQ=255)
|
AGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAA > minE/2595336‑2595474
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
