Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,595,427 |
A→C |
20.8% |
S391A (TCG→GCG) ‡ |
arsB ← |
arsenite/antimonite transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,595,427 | 0 | A | C | 20.8%
| 203.1
/ 69.2
| 125 | S391A (TCG→GCG) ‡ | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base A (60/39); new base C (10/16); total (70/55) |
| Fisher's exact test for biased strand distribution p-value = 4.88e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 129 total aligned reads displayed.
AGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCA > minE/2595362‑2595497
|
aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa > 1:1731795/1‑71 (MQ=255)
ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc < 1:4430395/71‑1 (MQ=255)
gcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg < 1:5252685/71‑1 (MQ=255)
caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt < 1:4120927/71‑1 (MQ=255)
caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt < 1:1486229/71‑1 (MQ=255)
acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCAc < 1:1809577/65‑1 (MQ=255)
acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:555069/68‑1 (MQ=255)
acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:5285263/68‑1 (MQ=255)
acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg > 1:1389504/4‑71 (MQ=255)
gggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGc < 1:1524422/71‑1 (MQ=255)
cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:2483560/68‑1 (MQ=255)
tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGaa < 1:5615276/59‑1 (MQ=255)
tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg > 1:774505/1‑61 (MQ=255)
tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg > 1:4417615/1‑61 (MQ=255)
cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGc > 1:3535977/1‑70 (MQ=255)
aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa < 1:5321996/71‑1 (MQ=255)
aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg > 1:833614/1‑65 (MQ=255)
aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa < 1:203005/71‑1 (MQ=255)
aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag < 1:5564881/71‑1 (MQ=255)
tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg > 1:5424356/1‑55 (MQ=255)
tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc > 1:4444805/1‑71 (MQ=255)
cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta > 1:516239/1‑68 (MQ=255)
cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta > 1:4924942/1‑68 (MQ=255)
cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta > 1:5274802/1‑68 (MQ=255)
cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta > 1:2801405/1‑68 (MQ=255)
ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg > 1:4551306/1‑70 (MQ=255)
cggTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagtt < 1:5530447/69‑2 (MQ=255)
tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag > 1:3436204/1‑71 (MQ=255)
gTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc > 1:4700516/1‑71 (MQ=255)
gTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCAcat < 1:2890320/52‑3 (MQ=255)
tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa > 1:1377180/1‑48 (MQ=255)
tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag < 1:846704/69‑1 (MQ=255)
accgAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc < 1:4689893/67‑1 (MQ=255)
aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc > 1:4250600/1‑69 (MQ=255)
ggAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg > 1:2068162/1‑71 (MQ=255)
aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc > 1:4607899/1‑70 (MQ=255)
aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc > 1:1061447/1‑70 (MQ=255)
aaGTAATATCCCCAAGTGATTGTCATATTTTTCTTGGCAAGCACATGCAGCCac < 1:3114598/54‑2 (MQ=255)
aaGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCac < 1:4735523/54‑2 (MQ=255)
aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtg > 1:2813062/1‑70 (MQ=255)
aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt < 1:1140045/70‑1 (MQ=255)
aGTAATATCCCCAAGTGATTGTCATATTTTTCAGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt < 1:878483/70‑1 (MQ=255)
gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:457075/45‑1 (MQ=255)
gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:1365411/45‑1 (MQ=255)
gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc < 1:1763288/71‑3 (MQ=255)
tAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc > 1:3296041/1‑68 (MQ=255)
aaTATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCa > 1:3256388/1‑45 (MQ=255)
atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg > 1:292915/1‑64 (MQ=255)
atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg > 1:5683652/1‑63 (MQ=255)
atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa > 1:2033974/1‑71 (MQ=255)
atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa < 1:5022952/70‑1 (MQ=255)
tatCCCCAGCTGATAGTCATATTCTTCTGCGaaat < 1:367818/35‑2 (MQ=255)
tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAg < 1:4743152/35‑1 (MQ=255)
tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCATAGTAGCGTAGCCAGGCTACCaa < 1:4347204/70‑1 (MQ=255)
tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt < 1:2615888/71‑1 (MQ=255)
tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg > 1:3338200/1‑71 (MQ=255)
cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg > 1:4068552/1‑71 (MQ=255)
cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg > 1:1531737/1‑71 (MQ=255)
cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga < 1:1632740/43‑1 (MQ=255)
cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga < 1:1476712/43‑1 (MQ=255)
cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga < 1:262241/43‑1 (MQ=255)
aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt > 1:2497030/1‑70 (MQ=255)
gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc > 1:1134345/1‑46 (MQ=255)
gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa < 1:1272417/71‑1 (MQ=255)
tGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCTAGGCTACCa < 1:2456311/59‑1 (MQ=255)
aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc > 1:124800/1‑47 (MQ=255)
aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAtt < 1:4232981/69‑1 (MQ=255)
tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag < 1:1191807/37‑1 (MQ=255)
aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta > 1:1767616/1‑43 (MQ=255)
aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta > 1:2896609/1‑43 (MQ=255)
aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt > 1:4653318/1‑57 (MQ=255)
aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg > 1:3891123/1‑71 (MQ=255)
aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg > 1:3583917/1‑71 (MQ=255)
gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg < 1:5434796/70‑1 (MQ=255)
tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCggg < 1:1234240/71‑2 (MQ=255)
atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa > 1:1007220/1‑43 (MQ=255)
atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa > 1:3477133/1‑43 (MQ=255)
atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt > 1:979338/1‑64 (MQ=255)
atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc > 1:61438/1‑66 (MQ=255)
atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc > 1:2489482/1‑66 (MQ=255)
atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc > 1:866379/1‑66 (MQ=255)
atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc > 1:3251462/1‑71 (MQ=255)
atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc > 1:4548297/1‑71 (MQ=255)
tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc > 1:4974575/1‑70 (MQ=255)
ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa < 1:4267265/71‑1 (MQ=255)
tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa < 1:4705840/39‑1 (MQ=255)
tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa < 1:1290473/47‑1 (MQ=255)
tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc > 1:1699749/1‑68 (MQ=255)
cttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaaa < 1:4285372/70‑1 (MQ=255)
ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc < 1:1002196/40‑1 (MQ=255)
ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc > 1:1011515/1‑40 (MQ=255)
ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc > 1:696216/1‑71 (MQ=255)
gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg > 1:4940678/1‑71 (MQ=255)
gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg > 1:2687148/1‑71 (MQ=255)
gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg > 1:3120286/1‑71 (MQ=255)
cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc < 1:2793191/68‑1 (MQ=255)
cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc < 1:2964023/68‑1 (MQ=255)
gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc < 1:3178037/65‑1 (MQ=255)
gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc > 1:3121949/1‑70 (MQ=255)
aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa < 1:1807966/71‑1 (MQ=255)
|
AGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCA > minE/2595362‑2595497
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
