Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,514 C→T 29.4% V362I (GTT→ATT)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,5140CT29.4% 293.4 / 146.9 204V362I (GTT→ATT) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base C (79/65);  new base T (46/14);  total (125/79)
Fisher's exact test for biased strand distribution p-value = 4.35e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01

Only 100 of 211 total aligned reads displayed.

AGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCG  >  minE/2595444‑2595582
                                                                      |                                                                    
agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc                                                                      <  1:5198531/71‑1 (MQ=255)
       ttgcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCAt                                                                   >  1:308917/3‑67 (MQ=255)
       tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCAt                                                                   <  1:2479251/67‑1 (MQ=255)
        agcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGct                                                               >  1:117541/1‑70 (MQ=255)
        agcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAACCGCAGCCAATCACATTGGCATAAACCATCGctt                                                              >  1:1749143/1‑71 (MQ=255)
         gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctc                                                             >  1:4111743/1‑71 (MQ=255)
          ccAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAcc                                                                     <  1:1278617/62‑1 (MQ=255)
            aGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCttt                                                          <  1:1136169/71‑1 (MQ=255)
              gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa                                                        >  1:44154/1‑71 (MQ=255)
                tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCg                                                                 >  1:4759544/1‑60 (MQ=255)
                tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt                                                       >  1:2367820/1‑70 (MQ=255)
                    aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCa                                                                    >  1:1377362/1‑53 (MQ=255)
                    aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc                                                  <  1:2905130/71‑1 (MQ=255)
                      ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAcc                                                                     <  1:5342575/50‑1 (MQ=255)
                      ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc                                                  >  1:3177689/1‑69 (MQ=255)
                        ggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAg                                               <  1:3745095/70‑1 (MQ=255)
                        ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGcttctt                                                           >  1:2215367/1‑58 (MQ=255)
                        ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa                                                     >  1:2880212/1‑64 (MQ=255)
                        ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa                                                     >  1:623043/1‑64 (MQ=255)
                        ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc                                                  >  1:4763680/1‑67 (MQ=255)
                         gggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgtc                                             >  1:3301054/1‑69 (MQ=255)
                         gggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGa                                              <  1:720008/70‑1 (MQ=255)
                         gggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGa                                              <  1:2410889/70‑1 (MQ=255)
                           gTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTg                                                         <  1:4444327/57‑1 (MQ=255)
                            tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc                                          <  1:848510/71‑1 (MQ=255)
                                tttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGACAGATGCCGtg                                       <  1:1286167/70‑1 (MQ=255)
                                tttCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtac                                      >  1:4252284/1‑69 (MQ=255)
                                tttCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtac                                      >  1:947643/1‑69 (MQ=255)
                                 ttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct                                     >  1:1945590/1‑71 (MQ=255)
                                  tCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct                                     >  1:803359/1‑70 (MQ=255)
                                     gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCttt                                                          >  1:2205122/1‑46 (MQ=255)
                                      tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAt                                >  1:5619693/1‑71 (MQ=255)
                                       cctaaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTc                               <  1:2033291/68‑1 (MQ=255)
                                       cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc                                          >  1:5393428/1‑60 (MQ=255)
                                       cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc                                          >  1:3866857/1‑60 (MQ=255)
                                       cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAt                                >  1:3396011/1‑70 (MQ=255)
                                        ccAAATCGCAGCCAATCACATTGGCATAAACCATCGcttct                                                            <  1:3713674/41‑1 (MQ=255)
                                        ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCttt                                                          <  1:5252944/43‑1 (MQ=255)
                                        ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc                               <  1:3949922/70‑1 (MQ=255)
                                        ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc                               <  1:1722350/70‑1 (MQ=255)
                                        ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc                               <  1:3367170/70‑1 (MQ=255)
                                        ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc                               <  1:5299032/70‑1 (MQ=255)
                                         taaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCaa                             >  1:3565030/2‑71 (MQ=255)
                                         cAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa                             <  1:3422265/71‑1 (MQ=255)
                                          aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt                            >  1:2927406/1‑71 (MQ=255)
                                          aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt                            <  1:833011/71‑1 (MQ=255)
                                          aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt                            >  1:4870513/1‑71 (MQ=255)
                                           aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg                                         <  1:131323/57‑1 (MQ=255)
                                           aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                           <  1:334155/71‑1 (MQ=255)
                                           aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc                           <  1:5437133/71‑2 (MQ=255)
                                             tcacAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                         >  1:2392320/4‑71 (MQ=255)
                                             tCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa                         >  1:4288879/1‑71 (MQ=255)
                                              cacAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAg                                               >  1:5465245/3‑48 (MQ=255)
                                               gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc                        <  1:3255432/70‑1 (MQ=255)
                                               gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc                        <  1:871666/70‑1 (MQ=255)
                                               acAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACa                       <  1:214923/70‑1 (MQ=255)
                                                cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa                      <  1:770904/71‑1 (MQ=255)
                                                 aGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt                                        <  1:2690013/52‑1 (MQ=255)
                                                  gCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg                    >  1:4631505/1‑71 (MQ=255)
                                                   ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc                                      >  1:416460/1‑52 (MQ=255)
                                                   ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                   >  1:4701257/1‑71 (MQ=255)
                                                      aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                         >  1:1608917/1‑62 (MQ=255)
                                                      aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                         >  1:3782538/1‑62 (MQ=255)
                                                      aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                         >  1:2176540/1‑62 (MQ=255)
                                                      aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                         >  1:2777726/1‑62 (MQ=255)
                                                      aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                         >  1:595489/1‑62 (MQ=255)
                                                      aTCACATTGGCATAAACCATCGCTTCTTTGATAACGcc                                                 <  1:553610/38‑1 (MQ=255)
                                                      aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc                <  1:5071896/71‑1 (MQ=255)
                                                      aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc                <  1:2079645/71‑1 (MQ=255)
                                                       tCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                         >  1:4010419/1‑61 (MQ=255)
                                                       tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGa                                              >  1:2980187/1‑40 (MQ=255)
                                                        cacaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta                                       >  1:2658977/1‑45 (MQ=255)
                                                        cacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc                                   >  1:1351659/1‑50 (MQ=255)
                                                        cacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa              >  1:464410/1‑71 (MQ=255)
                                                         acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca                  >  1:223661/1‑65 (MQ=255)
                                                         acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc             >  1:157761/1‑71 (MQ=255)
                                                         acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc             >  1:3459158/1‑71 (MQ=255)
                                                         acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc             <  1:1037860/71‑1 (MQ=255)
                                                          caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc            <  1:1634087/71‑1 (MQ=255)
                                                           aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa                         >  1:168534/1‑57 (MQ=255)
                                                            ttGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt                            >  1:3608310/1‑53 (MQ=255)
                                                            ttGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt                            >  1:2988091/1‑53 (MQ=255)
                                                            ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg          <  1:3909527/71‑1 (MQ=255)
                                                            ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg          <  1:1979696/71‑1 (MQ=255)
                                                             tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa              >  1:3055542/1‑66 (MQ=255)
                                                              ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa                             >  1:3308327/1‑50 (MQ=255)
                                                              ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt         <  1:1822723/70‑1 (MQ=255)
                                                              ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt         <  1:1472548/70‑1 (MQ=255)
                                                                  tAAACCATCGCTTCTTTGATAACGCCAGATGCCGt                                        <  1:2900094/35‑1 (MQ=255)
                                                                  tAAACCATCGCTTCTTTGATAACGCCAGATGCCGt                                        <  1:2747557/35‑1 (MQ=255)
                                                                   aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc       >  1:1249439/1‑67 (MQ=255)
                                                                   aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc       >  1:1088319/1‑67 (MQ=255)
                                                                   aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc   >  1:2560031/1‑71 (MQ=255)
                                                                    aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                  >  1:5035245/1‑55 (MQ=255)
                                                                    aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                  >  1:2718291/1‑55 (MQ=255)
                                                                    aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg  >  1:4571849/1‑71 (MQ=255)
                                                                    aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg  >  1:4005558/1‑71 (MQ=255)
                                                                    aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg  >  1:190643/1‑71 (MQ=255)
                                                                    aaCCATCGCTTCTTTGATAACGCCAGATGCCGAGCTGCCATCAATGGACAACGCGCCAAcc            >  1:4243777/1‑61 (MQ=255)
                                                                    aaCCATAGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                  >  1:3215243/1‑55 (MQ=255)
                                                                      |                                                                    
AGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCG  >  minE/2595444‑2595582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A