Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,557 G→C 25.0% S347S (TCC→TCG)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,5570GC25.0% 264.4 / 96.1 152S347S (TCC→TCG) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (61/53);  new base C (23/15);  total (84/68)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01

Only 100 of 155 total aligned reads displayed.

AATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATC  >  minE/2595487‑2595624
                                                                      |                                                                   
aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                                     <  1:334155/71‑1 (MQ=255)
  tCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa                                                                   >  1:4288879/1‑71 (MQ=255)
  tCGCAGCCAATCACATTGGCATAAACAATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa                                                                   >  1:167266/1‑71 (MQ=255)
    gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc                                                                  <  1:4587681/70‑1 (MQ=255)
    gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc                                                                  <  1:871666/70‑1 (MQ=255)
    gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc                                                                  <  1:3386210/70‑1 (MQ=255)
    gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc                                                                  <  1:3255432/70‑1 (MQ=255)
    acAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                                                                   >  1:2101087/2‑69 (MQ=255)
    acAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGAc                                                                  <  1:3159032/69‑1 (MQ=255)
    acAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGAc                                                                  <  1:1526969/69‑1 (MQ=255)
     cAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACag                                                                <  1:1179477/71‑2 (MQ=255)
     cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa                                                                <  1:770904/71‑1 (MQ=255)
       gCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg                                                              <  1:2046393/71‑1 (MQ=255)
       gCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcg                                                              <  1:2923227/71‑1 (MQ=255)
        ccAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgc                                                             <  1:3262228/71‑1 (MQ=255)
        ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                                                             >  1:4701257/1‑71 (MQ=255)
           aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                                                                   >  1:2176540/1‑62 (MQ=255)
           aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                                                                   >  1:595489/1‑62 (MQ=255)
           aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                                                                   >  1:66522/1‑62 (MQ=255)
           aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                                                                   >  1:2181547/1‑62 (MQ=255)
           aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                                                                   >  1:2777726/1‑62 (MQ=255)
           aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                                                                   >  1:1608917/1‑62 (MQ=255)
           aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc                                                          <  1:2079645/71‑1 (MQ=255)
             cacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATgg                                                                    >  1:5564043/1‑59 (MQ=255)
             cacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa                                                        >  1:464410/1‑71 (MQ=255)
             cacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa                                                        >  1:5013667/1‑71 (MQ=255)
              acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca                                                            >  1:223661/1‑65 (MQ=255)
              acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca                                                            >  1:3832121/1‑65 (MQ=255)
              acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa                                                        <  1:466127/70‑1 (MQ=255)
              acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                       >  1:2308503/1‑71 (MQ=255)
              acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                       >  1:3459158/1‑71 (MQ=255)
              acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                       <  1:5472608/71‑1 (MQ=255)
              acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                       >  1:157761/1‑71 (MQ=255)
               caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                                      <  1:1634087/71‑1 (MQ=255)
               caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                       <  1:1966156/70‑1 (MQ=255)
                aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa                                                                   >  1:5175147/1‑57 (MQ=255)
                aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAc                                                               <  1:2983404/61‑1 (MQ=255)
                aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                                     <  1:4849085/71‑1 (MQ=255)
                 ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                                    <  1:1979696/71‑1 (MQ=255)
                 ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                                    <  1:3909527/71‑1 (MQ=255)
                  tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa                                                                   >  1:1689067/1‑55 (MQ=255)
                  tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa                                                                   >  1:2541286/1‑55 (MQ=255)
                    gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa                                                  <  1:3422995/70‑1 (MQ=255)
                    gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa                                                  <  1:2396974/70‑1 (MQ=255)
                       tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                                     <  1:135212/48‑1 (MQ=255)
                       tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                                              >  1:1124572/1‑71 (MQ=255)
                        aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc                                                 >  1:1249439/1‑67 (MQ=255)
                        aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc                                                 >  1:1088319/1‑67 (MQ=255)
                         aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa                                                                >  1:2762815/1‑51 (MQ=255)
                         aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                                                            >  1:2718291/1‑55 (MQ=255)
                         aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                                                            >  1:3736814/1‑55 (MQ=255)
                         aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                                                            >  1:5035245/1‑55 (MQ=255)
                         aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                             <  1:4005571/70‑1 (MQ=255)
                         aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                            >  1:4005558/1‑71 (MQ=255)
                         aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                            >  1:1911825/1‑71 (MQ=255)
                         aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                            >  1:4571849/1‑71 (MQ=255)
                         aaCCATCGCTTCTTTGATAACGCCAGATGCCGAGCTGCCATCAATGGACAACGCGCCAAcc                                                      >  1:4243777/1‑61 (MQ=255)
                         aaCCATAGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                                                            >  1:3215243/1‑55 (MQ=255)
                                gcctcttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCatat                                      >  1:300896/4‑70 (MQ=255)
                                gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                                     >  1:285528/1‑71 (MQ=255)
                                 cctcttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg                                    <  1:4234961/69‑1 (MQ=255)
                                    cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                                                             <  1:5218190/43‑1 (MQ=255)
                                    cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                                   <  1:1743118/69‑1 (MQ=255)
                                      ttGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                                           >  1:1388473/1‑43 (MQ=255)
                                      ttGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                                           >  1:2577139/1‑43 (MQ=255)
                                       tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat                               <  1:278853/70‑1 (MQ=255)
                                        gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                             >  1:467426/1‑71 (MQ=255)
                                         aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                                   >  1:5610185/1‑64 (MQ=255)
                                            aCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                               <  1:5301797/65‑1 (MQ=255)
                                            aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATaga                         <  1:1448564/71‑1 (MQ=255)
                                            aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                          <  1:1061604/70‑1 (MQ=255)
                                            aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                          <  1:5685742/70‑1 (MQ=255)
                                            aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                          <  1:364533/70‑1 (MQ=255)
                                             cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                        <  1:3890566/71‑1 (MQ=255)
                                             cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                        <  1:464555/71‑1 (MQ=255)
                                              gCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCatca                             >  1:1893540/1‑63 (MQ=255)
                                              gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaag                       <  1:1003764/71‑1 (MQ=255)
                                               ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                                                <  1:2483012/45‑1 (MQ=255)
                                                     gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                             >  1:5313032/1‑42 (MQ=255)
                                                     gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                             >  1:1026562/1‑42 (MQ=255)
                                                     gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg                                    >  1:3789575/1‑51 (MQ=255)
                                                     gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGt                                   >  1:2675742/1‑52 (MQ=255)
                                                      ccGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGtt                                  >  1:4158798/1‑52 (MQ=255)
                                                      ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                               <  1:1134433/55‑1 (MQ=255)
                                                       cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg              >  1:4332174/1‑69 (MQ=255)
                                                         tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCgg                                           <  1:3779870/40‑1 (MQ=255)
                                                         tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCg            <  1:2098620/71‑1 (MQ=255)
                                                           ctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCat                                        >  1:569487/1‑41 (MQ=255)
                                                              acATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAg       >  1:2566778/2‑71 (MQ=255)
                                                               cgtCAATCGACAGCGCACCAATCAGTACCGTCGGCATATTGTTCat                               >  1:1992271/3‑46 (MQ=25)
                                                               cATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                             >  1:3032727/1‑48 (MQ=255)
                                                                 tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                                    <  1:2668579/39‑1 (MQ=255)
                                                                 tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                                    <  1:1539955/39‑1 (MQ=255)
                                                                  cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt   >  1:606189/1‑71 (MQ=255)
                                                                   aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag                     >  1:2789313/1‑52 (MQ=255)
                                                                   aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc  <  1:3490046/71‑3 (MQ=255)
                                                                   aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc  <  1:4929070/71‑3 (MQ=255)
                                                                    aTGGACAACCCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc  >  1:3660895/1‑70 (MQ=255)
                                                                     tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                          <  1:2407353/45‑1 (MQ=255)
                                                                     tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt          <  1:218872/61‑1 (MQ=255)
                                                                      |                                                                   
AATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATC  >  minE/2595487‑2595624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A