Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,595,575 |
T→C |
23.4% |
V341V (GTA→GTG) |
arsB ← |
arsenite/antimonite transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,595,575 | 0 | T | C | 23.4%
| 162.9
/ 50.3
| 94 | V341V (GTA→GTG) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base T (40/32); new base C (8/14); total (48/46) |
| Fisher's exact test for biased strand distribution p-value = 1.46e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01 |
GGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGG > minE/2595506‑2595642
|
ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt < 1:4262823/70‑1 (MQ=255)
ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt < 1:1822723/70‑1 (MQ=255)
ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt < 1:1472548/70‑1 (MQ=255)
gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa < 1:2396974/70‑1 (MQ=255)
gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa < 1:3422995/70‑1 (MQ=255)
tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt > 1:1124572/1‑71 (MQ=255)
aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc > 1:1088319/1‑67 (MQ=255)
aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc > 1:1249439/1‑67 (MQ=255)
aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:2560031/1‑71 (MQ=255)
aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc < 1:4005571/70‑1 (MQ=255)
aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:4762948/1‑71 (MQ=255)
aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:1911825/1‑71 (MQ=255)
aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:190643/1‑71 (MQ=255)
aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:4005558/1‑71 (MQ=255)
aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:4571849/1‑71 (MQ=255)
gcctcttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCatat > 1:300896/4‑70 (MQ=255)
gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt > 1:285528/1‑71 (MQ=255)
gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt < 1:4176290/71‑1 (MQ=255)
cctcttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg < 1:4234961/69‑1 (MQ=255)
cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt < 1:1743118/69‑1 (MQ=255)
tttGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt < 1:1311838/69‑1 (MQ=255)
tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt > 1:389031/1‑55 (MQ=255)
tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat < 1:278853/70‑1 (MQ=255)
gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa > 1:467426/1‑71 (MQ=255)
gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa > 1:900912/1‑71 (MQ=255)
aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt > 1:5610185/1‑64 (MQ=255)
cgaCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTc < 1:2852098/63‑1 (MQ=255)
aCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat < 1:5301797/65‑1 (MQ=255)
aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATaga < 1:1448564/71‑1 (MQ=255)
aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATaga < 1:1441744/71‑1 (MQ=255)
aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:4980414/70‑1 (MQ=255)
aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:364533/70‑1 (MQ=255)
aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:1061604/70‑1 (MQ=255)
aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:5685742/70‑1 (MQ=255)
cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa < 1:464555/71‑1 (MQ=255)
cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa < 1:3890566/71‑1 (MQ=255)
gCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCatca > 1:1893540/1‑63 (MQ=255)
gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaag < 1:1003764/71‑1 (MQ=255)
ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc < 1:2483012/45‑1 (MQ=255)
ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga < 1:1625058/71‑1 (MQ=255)
gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag < 1:5283863/71‑1 (MQ=255)
gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:1142167/1‑42 (MQ=255)
gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:1026562/1‑42 (MQ=255)
gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:5313032/1‑42 (MQ=255)
gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:5695721/1‑42 (MQ=255)
gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:3029323/1‑42 (MQ=255)
gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg > 1:3789575/1‑51 (MQ=255)
gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGt > 1:2675742/1‑52 (MQ=255)
ccGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGtt > 1:4158798/1‑52 (MQ=255)
ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat < 1:4648604/55‑1 (MQ=255)
ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat < 1:5532385/55‑1 (MQ=255)
ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat < 1:1134433/55‑1 (MQ=255)
cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg > 1:4332174/1‑69 (MQ=255)
tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCgg < 1:3779870/40‑1 (MQ=255)
tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCgg < 1:2187126/40‑1 (MQ=255)
tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCg < 1:2098620/71‑1 (MQ=255)
ctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCat > 1:569487/1‑41 (MQ=255)
acATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAg > 1:2566778/2‑71 (MQ=255)
cgtCAATCGACAGCGCACCAATCAGTACCGTCGGCATATTGTTCat > 1:1992271/3‑46 (MQ=25)
cgtCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat > 1:566624/3‑46 (MQ=255)
cATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa > 1:3032727/1‑48 (MQ=255)
tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg < 1:1539955/39‑1 (MQ=255)
tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg < 1:3412685/39‑1 (MQ=255)
tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg < 1:2668579/39‑1 (MQ=255)
tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa < 1:4671686/71‑1 (MQ=255)
cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt > 1:606189/1‑71 (MQ=255)
aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata > 1:2973408/1‑43 (MQ=255)
aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata > 1:2090024/1‑43 (MQ=255)
aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata > 1:1108406/1‑43 (MQ=255)
aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata > 1:1973001/1‑43 (MQ=255)
aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag > 1:2789313/1‑52 (MQ=255)
aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc < 1:3490046/71‑3 (MQ=255)
aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc < 1:4929070/71‑3 (MQ=255)
aTGGACAACCCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc > 1:3660895/1‑70 (MQ=255)
tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:2407353/45‑1 (MQ=255)
tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:3300775/45‑1 (MQ=255)
tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:4363712/45‑1 (MQ=255)
tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:3637119/45‑1 (MQ=255)
tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt < 1:218872/61‑1 (MQ=255)
gACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt < 1:3523388/71‑1 (MQ=255)
gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa > 1:1057806/1‑53 (MQ=255)
cAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc > 1:3578976/1‑71 (MQ=255)
gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg < 1:3499369/70‑1 (MQ=255)
gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg < 1:4148179/70‑1 (MQ=255)
cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga > 1:2020014/1‑42 (MQ=255)
cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga > 1:800238/1‑42 (MQ=255)
cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga > 1:5598692/1‑42 (MQ=255)
cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:1755250/1‑65 (MQ=255)
accAATCAGCACCGTCGGCATATTGTTCATGACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGaac < 1:4954810/69‑3 (MQ=255)
accAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACg > 1:5332894/2‑71 (MQ=255)
ccAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt > 1:1209201/1‑71 (MQ=255)
cAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc < 1:5586629/71‑1 (MQ=255)
cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt < 1:4575829/70‑1 (MQ=255)
cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTc > 1:5083682/1‑71 (MQ=255)
aatcAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTcg > 1:1122560/4‑71 (MQ=255)
aaCCAGTACCGTCGGCATATTGTTCATAATagaaga < 1:579353/36‑1 (MQ=255)
cAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCgg > 1:4912015/1‑71 (MQ=255)
|
GGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGG > minE/2595506‑2595642
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
