Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,595,623 |
T→G |
37.3% |
G325G (GGA→GGC) |
arsB ← |
arsenite/antimonite transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,595,623 | 0 | T | G | 37.3%
| 14.2
/ 47.2
| 43 | G325G (GGA→GGC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base T (15/12); new base G (6/10); total (21/22) |
| Fisher's exact test for biased strand distribution p-value = 3.47e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 |
CAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCC > minE/2595553‑2595692
|
cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt > 1:606189/1‑71 (MQ=255)
aTGGACAACCCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc > 1:3660895/1‑70 (MQ=255)
gACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt < 1:3523388/71‑1 (MQ=255)
cAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc > 1:3578976/1‑71 (MQ=255)
gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg < 1:4148179/70‑1 (MQ=255)
gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg < 1:3499369/70‑1 (MQ=255)
cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:1755250/1‑65 (MQ=255)
accAATCAGCACCGTCGGCATATTGTTCATGACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGaac < 1:4954810/69‑3 (MQ=255)
accAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACg > 1:5332894/2‑71 (MQ=255)
ccAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt > 1:1209201/1‑71 (MQ=255)
cAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc < 1:5586629/71‑1 (MQ=255)
cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt < 1:4575829/70‑1 (MQ=255)
cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTc > 1:5083682/1‑71 (MQ=255)
aatcAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTcg > 1:1122560/4‑71 (MQ=255)
cAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCgg > 1:4912015/1‑71 (MQ=255)
aCCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc < 1:3247073/70‑1 (MQ=255)
ccGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCca < 1:2492122/70‑1 (MQ=255)
cGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata < 1:86341/71‑3 (MQ=255)
gTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata > 1:1935347/1‑68 (MQ=255)
gTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAg < 1:1573231/71‑1 (MQ=255)
tCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcataa > 1:4628111/1‑67 (MQ=255)
ggCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGt < 1:836104/56‑1 (MQ=255)
ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGcc > 1:1587922/1‑71 (MQ=255)
gCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcat < 1:3142045/65‑2 (MQ=255)
tatTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc > 1:2083290/1‑60 (MQ=255)
ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:3211892/1‑40 (MQ=255)
ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:5231212/1‑40 (MQ=255)
ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:5356225/1‑40 (MQ=255)
ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc > 1:4249838/1‑71 (MQ=255)
ttCATAATAGAAGAGGGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTa < 1:2715517/66‑1 (MQ=255)
aataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCa > 1:1830576/1‑71 (MQ=255)
ataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCAc > 1:1467928/1‑71 (MQ=255)
aagaGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGtt < 1:4530913/70‑1 (MQ=255)
tgagagaAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCa < 1:4123460/64‑1 (MQ=255)
tgagagaAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCa < 1:580369/64‑1 (MQ=255)
gagagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAg < 1:1046953/71‑1 (MQ=255)
gagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTa > 1:993494/1‑71 (MQ=255)
ggTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGaaa < 1:2801304/71‑1 (MQ=255)
gTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAg < 1:3897867/71‑1 (MQ=255)
gTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAg < 1:3026881/71‑1 (MQ=255)
aagAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCAGATTCAGCACGCCAGACAGgta > 1:3969711/3‑71 (MQ=255)
aagAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCAGATTCAGCACGCCAGACAGgta > 1:373104/3‑71 (MQ=255)
aGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACg < 1:4723700/51‑1 (MQ=255)
aTCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTcc < 1:2700788/71‑1 (MQ=255)
tCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGaaa > 1:4175024/1‑62 (MQ=255)
tCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGata > 1:4961856/1‑66 (MQ=255)
|
CAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCC > minE/2595553‑2595692
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
