Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,727 G→T 30.0% L291M (CTG→ATG) ‡ arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,7270GT30.0% 229.8 / 84.7 130L291M (CTG→ATG) ‡arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (44/47);  new base T (18/21);  total (62/68)
Fisher's exact test for biased strand distribution p-value = 8.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

Only 100 of 130 total aligned reads displayed.

CGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCG  >  minE/2595659‑2595795
                                                                    |                                                                    
cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                                                     <  1:2578486/70‑1 (MQ=255)
cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                                                     <  1:5487768/70‑1 (MQ=255)
cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                                                     <  1:237230/70‑1 (MQ=255)
cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                                                                    <  1:4913772/71‑1 (MQ=255)
  ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATa                                                                   <  1:5692575/70‑1 (MQ=255)
    aGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACAt                                                                >  1:619326/1‑71 (MQ=255)
        cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                                                            <  1:2684351/71‑1 (MQ=255)
        cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                                                            <  1:4507846/71‑1 (MQ=255)
                aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa                                                    <  1:3268683/71‑1 (MQ=255)
                aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa                                                    <  1:434414/71‑1 (MQ=255)
                 cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag                                                      >  1:3286343/1‑68 (MQ=255)
                  gCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaaaa                                                  <  1:951590/71‑3 (MQ=255)
                   ccAGAAAGATATTCCGTTAATCCGGAATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga                                                  >  1:4739359/1‑70 (MQ=255)
                     aGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa                                               <  1:3853428/71‑1 (MQ=255)
                       aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                                                    >  1:5593260/1‑64 (MQ=255)
                       aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                                                    >  1:1902425/1‑64 (MQ=255)
                         aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                           >  1:5290268/1‑71 (MQ=255)
                           atatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag                                                   <  1:3366957/61‑1 (MQ=255)
                             atTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                                 >  1:4202109/1‑61 (MQ=255)
                              ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc                                                         <  1:343597/52‑1 (MQ=255)
                              ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                      <  1:5425102/71‑1 (MQ=255)
                              ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                       <  1:3432388/70‑1 (MQ=255)
                                ccGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg                                    >  1:3361955/1‑71 (MQ=255)
                                ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGcc                                                             <  1:1361447/46‑1 (MQ=255)
                                ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGc                                       >  1:1502745/1‑68 (MQ=255)
                                 cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga                                                  >  1:3009797/1‑56 (MQ=255)
                                 cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                     <  1:2437817/69‑1 (MQ=255)
                                   ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag                                                      <  1:5134430/50‑1 (MQ=255)
                                     aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                <  1:5190734/70‑1 (MQ=255)
                                     aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                <  1:1520409/70‑1 (MQ=255)
                                       tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                               <  1:553044/69‑1 (MQ=255)
                                       tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                               <  1:395201/69‑1 (MQ=255)
                                       tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                               <  1:177354/69‑1 (MQ=255)
                                         cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt                                         <  1:4706119/57‑1 (MQ=255)
                                            cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                          >  1:4682640/1‑53 (MQ=255)
                                            cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                          >  1:5413957/1‑53 (MQ=255)
                                            cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                         >  1:311521/1‑70 (MQ=255)
                                            cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                         <  1:4448805/70‑1 (MQ=255)
                                            cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                         >  1:1359999/1‑70 (MQ=255)
                                            cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg                         >  1:4283970/1‑70 (MQ=255)
                                            cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg                         >  1:5508891/1‑70 (MQ=255)
                                            cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg                         >  1:3923888/1‑70 (MQ=255)
                                              ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTgaga                                                     <  1:5646928/40‑1 (MQ=255)
                                              ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTgaga                                                     <  1:4334479/40‑1 (MQ=255)
                                              ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTgaga                                                     <  1:628327/40‑1 (MQ=255)
                                              ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCa                       >  1:1753101/1‑70 (MQ=255)
                                              ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag                                                   <  1:5193117/42‑1 (MQ=255)
                                               tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg                      >  1:4959400/1‑70 (MQ=255)
                                               tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                             <  1:4538676/47‑1 (MQ=255)
                                               tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                             <  1:1414141/47‑1 (MQ=255)
                                               tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                             <  1:4774993/47‑1 (MQ=255)
                                               tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                             <  1:5031492/47‑1 (MQ=255)
                                                gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGAt                                           >  1:4017813/1‑48 (MQ=255)
                                                gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                          >  1:5028236/1‑49 (MQ=255)
                                                gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCAtggc                                 >  1:4981360/1‑57 (MQ=255)
                                                gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAgg                     <  1:1432804/70‑1 (MQ=255)
                                                gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGa                    >  1:2553886/1‑71 (MQ=255)
                                                gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                                 >  1:4180699/1‑42 (MQ=255)
                                                gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                            >  1:1478264/1‑47 (MQ=255)
                                                gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                            >  1:5657266/1‑47 (MQ=255)
                                                gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                          >  1:838677/1‑49 (MQ=255)
                                                gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                    >  1:5474520/1‑71 (MQ=255)
                                                 cgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGAc                   <  1:3725958/71‑1 (MQ=255)
                                                    aGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt                <  1:3163220/71‑1 (MQ=255)
                                                     ggCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt                 <  1:2847733/69‑1 (MQ=255)
                                                      gCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAc              >  1:3936944/1‑71 (MQ=255)
                                                       ccATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtttt               >  1:3117455/1‑68 (MQ=255)
                                                       ccATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtttt               >  1:176605/1‑68 (MQ=255)
                                                       ccATAAACCACCAGATACATGCCGAGCGAGAAGATGa                                               >  1:1987143/1‑37 (MQ=255)
                                                       ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                             <  1:5705/39‑1 (MQ=255)
                                                       ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                             <  1:4000200/39‑1 (MQ=255)
                                                       ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                             <  1:5257019/39‑1 (MQ=255)
                                                        cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                   >  1:1163787/1‑48 (MQ=255)
                                                         aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                   <  1:192852/63‑1 (MQ=255)
                                                         aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                   <  1:4301593/63‑1 (MQ=255)
                                                         aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                   <  1:786349/63‑1 (MQ=255)
                                                         aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                   <  1:3387899/63‑1 (MQ=255)
                                                         aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                   <  1:1097056/63‑1 (MQ=255)
                                                         aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTccgg           <  1:906572/71‑3 (MQ=255)
                                                         aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                       <  1:3889169/43‑1 (MQ=255)
                                                         aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                       <  1:3435103/43‑1 (MQ=255)
                                                         aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg                                    >  1:5338130/1‑46 (MQ=255)
                                                          tAAACCACCAGATATATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                <  1:4049902/49‑1 (MQ=255)
                                                          tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                       <  1:3462397/42‑1 (MQ=255)
                                                          tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc            <  1:299542/69‑1 (MQ=255)
                                                           aaaCCACCAGATACATGCCGAGCGAGAAGATGACAAt                                           >  1:2089697/1‑37 (MQ=255)
                                                            aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                     >  1:1519448/1‑42 (MQ=255)
                                                            aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                     >  1:4664768/1‑42 (MQ=255)
                                                            aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                            >  1:5229832/1‑51 (MQ=255)
                                                            aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                            >  1:1532783/1‑51 (MQ=255)
                                                             accaccAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAtt       <  1:719264/71‑1 (MQ=255)
                                                              ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc                                       >  1:1349049/1‑38 (MQ=255)
                                                              ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc                                       >  1:1025090/1‑38 (MQ=255)
                                                              ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc                                       >  1:945133/1‑38 (MQ=255)
                                                               caccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa     <  1:257140/71‑1 (MQ=255)
                                                                 ccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATc   <  1:2879642/71‑1 (MQ=255)
                                                                  caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg  <  1:3722434/71‑1 (MQ=255)
                                                                   aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg                      >  1:234510/1‑50 (MQ=255)
                                                                   aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg                      >  1:1036405/1‑50 (MQ=255)
                                                                   aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg  >  1:4186020/1‑70 (MQ=255)
                                                                    |                                                                    
CGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCG  >  minE/2595659‑2595795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A