Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,596,151 G→C 33.3% T149T (ACC→ACG)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,596,1510GC33.3% 76.2 / 58.0 69T149T (ACC→ACG) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (15/31);  new base C (10/13);  total (25/44)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.92e-01

GAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAG  >  minE/2596081‑2596219
                                                                      |                                                                    
gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCg                                                                      <  1:3270281/71‑1 (MQ=255)
 aaaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTTGAGACAATAAGCGGCAGGCTGGCgg                                                                     >  1:2759706/1‑71 (MQ=255)
   aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTa                                                                   >  1:4325507/1‑71 (MQ=255)
           ccgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCaa                                                            <  1:4561973/67‑1 (MQ=255)
           ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt                                                           <  1:61551/71‑1 (MQ=255)
            cgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAta                                                          <  1:1027594/69‑2 (MQ=255)
            cgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAt                                                           >  1:568668/3‑70 (MQ=255)
             gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa                                                         <  1:2907473/70‑3 (MQ=255)
             gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa                                                         <  1:1074104/70‑3 (MQ=255)
             aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCa                                                             <  1:254872/67‑1 (MQ=255)
             aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGa                                                         <  1:2147117/71‑1 (MQ=255)
              aagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGc                                                              <  1:2102362/65‑1 (MQ=255)
              aaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGaa                                                        <  1:1532620/71‑1 (MQ=255)
               aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt                                                       >  1:343780/1‑71 (MQ=255)
               aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt                                                       >  1:2210402/1‑71 (MQ=255)
                gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc                                                      >  1:8113/1‑71 (MQ=255)
                gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc                                                      <  1:1474192/71‑1 (MQ=255)
                gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc                                                      <  1:1231880/71‑1 (MQ=255)
                   aTCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCgg                                                   >  1:25208/1‑71 (MQ=255)
                     cAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg                                                                     >  1:3068564/1‑51 (MQ=255)
                        aggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc                                                              <  1:920563/54‑1 (MQ=255)
                          gagaCGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCAt                                            >  1:5038161/4‑71 (MQ=255)
                          gagaCGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCAt                                            >  1:618636/4‑71 (MQ=255)
                          gAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt                                            <  1:5361754/71‑1 (MQ=255)
                          gAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt                                            <  1:332899/71‑1 (MQ=255)
                              cGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACg                                        <  1:5620776/71‑1 (MQ=255)
                              cGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACg                                        <  1:1992297/71‑1 (MQ=255)
                              cGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAca                                        <  1:2088555/71‑2 (MQ=255)
                               gATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGgccgcc                                              <  1:1043670/64‑1 (MQ=255)
                               gATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGa                                       <  1:1032259/71‑1 (MQ=255)
                               gATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGa                                       <  1:1039141/71‑1 (MQ=255)
                                  atTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATc                                                      >  1:2517566/1‑53 (MQ=255)
                                   ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTAt                                                                  >  1:1198090/1‑40 (MQ=255)
                                   ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt                                                           <  1:86279/47‑1 (MQ=255)
                                   ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt                                                           <  1:5580356/47‑1 (MQ=255)
                                   ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc                                   <  1:2205322/71‑1 (MQ=255)
                                   ttCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGc                                   <  1:2890870/71‑1 (MQ=255)
                                   ttCACCAGCTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCAt                                            <  1:2379629/62‑1 (MQ=255)
                                     cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGcc                                  <  1:4629170/70‑1 (MQ=255)
                                     cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGcc                                  <  1:5543577/70‑1 (MQ=255)
                                     cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc                                   >  1:3401340/1‑69 (MQ=255)
                                     cACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGcc                                  <  1:2777360/70‑1 (MQ=255)
                                        cAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg                              <  1:1548326/71‑1 (MQ=255)
                                        cAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtg                              >  1:3791224/1‑69 (MQ=255)
                                         aGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt                                            >  1:3866034/1‑56 (MQ=255)
                                         aGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt                                            >  1:3513260/1‑56 (MQ=255)
                                         aGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCa                                 >  1:2594433/1‑67 (MQ=255)
                                          ggTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg                              >  1:4563082/1‑69 (MQ=255)
                                          ggTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgtc                            >  1:1878442/1‑70 (MQ=255)
                                          ggTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgtc                            >  1:2786185/1‑70 (MQ=255)
                                           gTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAcaa                                       <  1:4699307/59‑3 (MQ=255)
                                           gTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtg                              <  1:204203/68‑3 (MQ=255)
                                              ggAGACAATAAGCGGCAGGCTGGCGGTATCGGaaa                                                            >  1:995273/1‑35 (MQ=255)
                                               gagaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCg                                                    <  1:4551296/42‑1 (MQ=255)
                                               gagaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGcc                       <  1:2455605/71‑1 (MQ=255)
                                                 gaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCtt                     <  1:1011863/71‑1 (MQ=255)
                                                 gaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCtt                     <  1:5668941/71‑1 (MQ=255)
                                                 gaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCtt                     <  1:2713506/71‑1 (MQ=255)
                                                 gaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCtt                     <  1:3078189/71‑1 (MQ=255)
                                                  aCAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATcct                                                    <  1:3834837/39‑2 (MQ=21)
                                                  aCAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGccctg                    >  1:2704228/1‑68 (MQ=255)
                                                  aCAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGccctg                    <  1:1373634/71‑4 (MQ=255)
                                                   cAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCttt                    <  1:2206291/70‑1 (MQ=255)
                                                   cAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCttt                    <  1:2081361/70‑1 (MQ=255)
                                                      tAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACt                 >  1:3766970/1‑70 (MQ=255)
                                                        aGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGaa              <  1:679941/71‑1 (MQ=255)
                                                        aGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGa               <  1:3919204/70‑1 (MQ=255)
                                                        aGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATAACGaa                                      >  1:2443821/1‑47 (MQ=255)
                                                        aGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACaaa                                      >  1:4412467/1‑47 (MQ=255)
                                                        aGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGaa              >  1:1293074/1‑71 (MQ=255)
                                                                gCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAg      <  1:2946016/71‑1 (MQ=255)
                                                                    gCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCcag  >  1:1390934/1‑71 (MQ=255)
                                                                      |                                                                    
GAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAG  >  minE/2596081‑2596219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A