Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,918,751 A→G 23.8% D315D (GAT→GAC)  argI ← ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,918,7510AG23.8% 182.8 / 56.7 105D315D (GAT→GAC) argIornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand):  ref base A (40/40);  new base G (16/9);  total (56/49)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

ATAGCAATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAAT  >  minE/2918684‑2918820
                                                                   |                                                                     
aTAGCAATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATc                                                                     >  1:877360/1‑70 (MQ=255)
   gCAATTATTTACTGAGCGTCGCGCCCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCaaa                                                                  >  1:492088/1‑70 (MQ=255)
         attTACTGAGCGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATg                                                           >  1:2060654/1‑71 (MQ=255)
          tttACTGAGCGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATg                                                           <  1:644712/70‑1 (MQ=255)
          tttACTGAGCGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATAAAAAACAATg                                                           <  1:4485733/70‑1 (MQ=255)
            tACTGAGCGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCt                                                         <  1:5530519/70‑1 (MQ=255)
               caaGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTcgcc                                                     >  1:2189689/3‑70 (MQ=255)
                gAGCGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTgg                                                       <  1:5353396/68‑1 (MQ=255)
                aaGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTcg                                                                    <  1:5402266/54‑2 (MQ=255)
                aaGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTcgccg                                                    >  1:4261758/2‑69 (MQ=255)
                 aGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTcg                                                                    <  1:1345654/54‑2 (MQ=255)
                 aGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAacac                                                               <  1:1100466/59‑1 (MQ=255)
                 aGCGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTggcgg                                                    >  1:4924839/1‑70 (MQ=255)
                 aGCGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTggcgg                                                    <  1:4542014/70‑1 (MQ=255)
                 aGCGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTggcgg                                                    >  1:3020288/1‑70 (MQ=255)
                  gCGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCa                                                  <  1:4845447/71‑1 (MQ=255)
                   cGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCaaaaa                                                                >  1:757299/1‑56 (MQ=255)
                   cGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCaaaaa                                                                >  1:4725549/1‑56 (MQ=255)
                     gcgcgACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCa                                                  <  1:4935280/67‑1 (MQ=255)
                       gcgACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACa                                                              >  1:1689430/1‑54 (MQ=255)
                       gcgACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTc                                             >  1:526949/1‑71 (MQ=255)
                       gcgACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTc                                             >  1:5537665/1‑71 (MQ=255)
                        cgACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCg                                            <  1:1460990/71‑1 (MQ=255)
                          atcATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAacac                                                               <  1:5057015/48‑1 (MQ=255)
                           tcATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCaaac                                         <  1:2630418/70‑2 (MQ=255)
                           tcATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTCGTCGAACACGATGCTCGCCGCCGACTCaaac                                         <  1:4758191/70‑2 (MQ=255)
                            cATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTc                                             >  1:376165/1‑66 (MQ=255)
                            cATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGaa                                          >  1:1033733/1‑69 (MQ=255)
                            cATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCaaaca                                        >  1:3187916/1‑69 (MQ=255)
                            cATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCaaaca                                        >  1:3081639/1‑69 (MQ=255)
                            cATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCaaaca                                        >  1:1793959/1‑69 (MQ=255)
                             aTCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGAc                                       <  1:195660/71‑1 (MQ=255)
                             aTCACCGCTTTGATAGTATGCATACGGTTTTACGCCTGATCAAAAACAATGCTgg                                                       >  1:3327857/1‑55 (MQ=255)
                             aTCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCgaac                                                                 >  1:3876414/1‑44 (MQ=255)
                             aTCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCgaac                                                                 >  1:3346911/1‑44 (MQ=255)
                             aTCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCaaa                                          >  1:1322001/1‑68 (MQ=255)
                             aTCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAacac                                       >  1:1985400/1‑71 (MQ=255)
                                aCCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACa                                                              >  1:2851831/1‑45 (MQ=255)
                                aCCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCt                                     <  1:2302600/70‑1 (MQ=255)
                                aCCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCt                                     <  1:4749531/70‑1 (MQ=255)
                                aCCGCTTTGATAGTATGCATACGGTTTTCCG‑CTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCtca                                   >  1:5102262/1‑71 (MQ=255)
                                 ccGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCtca                                   <  1:447210/71‑1 (MQ=255)
                                 ccGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCtcg                                   >  1:2938459/1‑70 (MQ=255)
                                 ccGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCtcg                                   >  1:2910214/1‑70 (MQ=255)
                                  cGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCtcat                                  >  1:3119782/1‑71 (MQ=255)
                                  cGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTcg                                                                    <  1:295088/37‑2 (MQ=16)
                                   gCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCt                                                         <  1:4946635/47‑1 (MQ=255)
                                    cTTTGATAGTATTCATACGGTTTTCCGCCTGATCAAAAACaa                                                             >  1:4833172/1‑42 (MQ=255)
                                    cTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACaa                                                             >  1:306636/1‑42 (MQ=255)
                                     tttGATAGTATGCATACGGTTTTCCGCCTGATCAAAAAc                                                               <  1:1011809/39‑1 (MQ=255)
                                     tttGATAGTATGCATACGGTTTTCCGCCTGATCAAAAAc                                                               <  1:674663/39‑1 (MQ=255)
                                     tttGATAGTATGCATACGGTTTTCCGCCTGATCAAAAAc                                                               <  1:3987717/39‑1 (MQ=255)
                                     tttGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAg                               >  1:2536734/1‑71 (MQ=255)
                                       tGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGt                              <  1:961524/70‑1 (MQ=255)
                                       tGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGt                              <  1:1198552/70‑1 (MQ=255)
                                        gATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTg                             <  1:3663839/70‑1 (MQ=255)
                                        gATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTg                             <  1:2907587/70‑1 (MQ=255)
                                         aTAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGAc                           <  1:1445269/71‑1 (MQ=255)
                                         aTAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGAc                           >  1:1402036/1‑71 (MQ=255)
                                          tAGTATCCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACt                          <  1:5361374/71‑1 (MQ=255)
                                            gTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTc                        <  1:2452414/71‑1 (MQ=255)
                                              aTGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGaa                                          >  1:5548068/1‑51 (MQ=255)
                                              aTGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGaa                                          >  1:2568765/1‑51 (MQ=255)
                                               tGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCtcatca                                <  1:4097790/60‑1 (MQ=255)
                                               tGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCa                      >  1:4739036/1‑70 (MQ=255)
                                                 cATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCACCTCCAtcc                   >  1:3895276/1‑69 (MQ=255)
                                                  aTACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATAcc                  >  1:4716868/1‑71 (MQ=255)
                                                   tACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTc                        <  1:3943634/64‑1 (MQ=255)
                                                    ccGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCACCTCCATCCCGc                >  1:1221099/2‑71 (MQ=255)
                                                     cGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCACCTCCATCCCGc                >  1:4455993/1‑70 (MQ=255)
                                                     cGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGa                                                >  1:3950618/1‑38 (MQ=255)
                                                     cGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGGCCTCATCAg                               >  1:2695654/1‑55 (MQ=255)
                                                     cGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCtca                                   >  1:5146430/1‑51 (MQ=255)
                                                     cGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCtca                                   >  1:2192111/1‑51 (MQ=255)
                                                     cGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAg                               >  1:2339251/1‑55 (MQ=255)
                                                     cGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGc                >  1:2323733/1‑70 (MQ=255)
                                                     cGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGc                >  1:1584815/1‑70 (MQ=255)
                                                     cGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGc                >  1:4756248/1‑70 (MQ=255)
                                                     cGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGc                <  1:1832383/70‑1 (MQ=255)
                                                        ttttCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCACCTCCATCCCGCCGTg            <  1:818793/71‑1 (MQ=255)
                                                        ttttCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCACCTCCATCCCGCCGTg            <  1:2992857/71‑1 (MQ=255)
                                                        ttttCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATg            <  1:1080511/71‑1 (MQ=255)
                                                          ttCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATg            >  1:627066/1‑69 (MQ=255)
                                                          ttCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGt           <  1:5079649/70‑1 (MQ=255)
                                                          ttCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGt           <  1:1652947/70‑1 (MQ=255)
                                                          ttCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGt           <  1:132974/70‑1 (MQ=255)
                                                            ccGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTa          <  1:2975957/69‑1 (MQ=255)
                                                            ccGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAg         <  1:3210685/70‑1 (MQ=255)
                                                               ccTGATCAAAAACAATGCTGGCGGCAGATTCGAAGAc                                       >  1:3574575/1‑37 (MQ=255)
                                                               ccTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCa     <  1:4915402/71‑1 (MQ=255)
                                                                cTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCa     >  1:4355450/1‑70 (MQ=255)
                                                                cTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCa     >  1:1270875/1‑70 (MQ=255)
                                                                  gATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGAc                           <  1:1376599/46‑1 (MQ=255)
                                                                   aTCAAAAACAATGCTGGCGGCAGATTCGAAGACCtcat                                  <  1:2247571/38‑1 (MQ=255)
                                                                   aTCAAAAACAATGCTGGCGGCAGATTCGAAGACCtcat                                  <  1:2498425/38‑1 (MQ=255)
                                                                   aTCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTc                        <  1:879623/48‑1 (MQ=255)
                                                                   aTCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGt           <  1:3617310/61‑1 (MQ=255)
                                                                   aTCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTa          <  1:5396164/62‑1 (MQ=255)
                                                                   aTCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAAt  <  1:4201521/70‑1 (MQ=255)
                                                                   aTCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAAt  >  1:2346855/1‑70 (MQ=255)
                                                                   |                                                                     
ATAGCAATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAAT  >  minE/2918684‑2918820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A