Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,919,000 A→G 22.5% D232D (GAT→GAC)  argI ← ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,919,0000AG22.5% 204.4 / 56.0 111D232D (GAT→GAC) argIornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand):  ref base A (35/51);  new base G (10/15);  total (45/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

GATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCAC  >  minE/2918933‑2919067
                                                                   |                                                                   
gATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCg                                                                  >  1:3528390/1‑71 (MQ=255)
 atatTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCgg                                                                 >  1:1227457/1‑71 (MQ=255)
 atatTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCgg                                                                 <  1:1257813/71‑1 (MQ=255)
    ttCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGtat                                                              <  1:5155714/71‑1 (MQ=255)
    ttCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGtat                                                              <  1:2629652/71‑1 (MQ=255)
      cgcGCAGCAGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGTATAg                                                            <  1:2516815/69‑1 (MQ=255)
      cACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGtata                                                             <  1:4063829/70‑1 (MQ=255)
      cACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGta                                                               >  1:844675/1‑68 (MQ=255)
       gcGCAGCAGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGta                                                               >  1:4522728/2‑67 (MQ=255)
        cGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGa                                                           >  1:1364132/1‑70 (MQ=255)
         gcagcaGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGTATAGATa                                                         <  1:5687885/71‑1 (MQ=255)
         gcagcaGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGTATAGATa                                                         <  1:4846009/71‑1 (MQ=255)
         gcagcaATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATa                                                         <  1:3591582/71‑1 (MQ=255)
          cagcaATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATa                                                         >  1:3362456/1‑70 (MQ=255)
               atgcaatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATTGATaaa                                                       <  1:7510/67‑1 (MQ=255)
               atgcaatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGt                                                     >  1:5590788/1‑69 (MQ=255)
                tgcaatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAg                                                  <  1:2816233/71‑1 (MQ=255)
                 gcaatCCGTTCCGCCCATTTCTCTTTTGCTTCACCCATCGACACCCACACATCGGTATAGATAAAGTCAg                                                  >  1:2352198/1‑70 (MQ=255)
                 gcaatCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGtata                                                             >  1:4372634/1‑59 (MQ=255)
                 gcaatCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGta                                                               >  1:4387439/1‑57 (MQ=255)
                  caatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCcacaca                                                                     >  1:5061525/1‑50 (MQ=255)
                  caatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCa                                                   >  1:5453245/1‑68 (MQ=255)
                  caatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCa                                                >  1:2686812/1‑71 (MQ=255)
                    atCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCAcc                                              <  1:5682900/71‑1 (MQ=255)
                     tCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGc                                                 <  1:4840095/67‑1 (MQ=255)
                     tCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGc                                                 <  1:2691474/67‑1 (MQ=255)
                     tCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGc                                                 <  1:3498023/67‑1 (MQ=255)
                     tCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGc                                                 <  1:1403631/67‑1 (MQ=255)
                       cGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTc                                           <  1:70331/71‑1 (MQ=255)
                          tCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATaaa                                                       <  1:4610721/56‑1 (MQ=255)
                           ccGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAg                                                  >  1:1860339/1‑60 (MQ=255)
                            cGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGtat                                                              >  1:1695806/1‑47 (MQ=255)
                              cccATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGta                                                               >  1:4553246/1‑44 (MQ=255)
                              cccATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTccc                                    <  1:3060935/71‑1 (MQ=255)
                              cccATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTccc                                    <  1:3629874/71‑1 (MQ=255)
                              cccACTTCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGTATAGATAAAGTCCGCGCCCTTAAcgc                                      <  1:5240011/69‑3 (MQ=255)
                               ccATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCAc                                               >  1:5649250/1‑59 (MQ=255)
                                cATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGta                                                               >  1:202856/1‑42 (MQ=255)
                                cATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCtt                                  <  1:1871039/71‑1 (MQ=255)
                                 aTTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATaaa                                                       <  1:5070797/49‑1 (MQ=255)
                                   ttCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGc                                                 >  1:2339786/1‑53 (MQ=255)
                                   ttCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGc                                                 >  1:5249031/1‑53 (MQ=255)
                                   ttCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGc                                                 >  1:2054529/1‑53 (MQ=255)
                                   ttCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTcgc                               >  1:4237013/1‑71 (MQ=255)
                                   ttCTCTTTTGCTTCCCCCATAGACACCCACACATCGGTATAGATAAAGt                                                     >  1:1567805/1‑49 (MQ=255)
                                   ttCTCTTTGGCCTCGCCCATCGACACCCACACGTCgg                                                                 <  1:1492026/37‑1 (MQ=21)
                                   ttCTCTTTGGCCTCGCCCATCGACACCCACACGTCgg                                                                 <  1:1199738/37‑1 (MQ=21)
                                   ttCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGtata                                                             <  1:1836673/41‑1 (MQ=25)
                                    tctcTTTTGCTTCCCCCATCGACACCCACACATCGGta                                                               >  1:5175639/1‑38 (MQ=255)
                                    tctcTTTTGCTTCCCCCATCGACACCCACACATCGGta                                                               >  1:5169201/1‑38 (MQ=255)
                                    tctcTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTc                                                    <  1:743526/49‑1 (MQ=255)
                                    tctcTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTc                                                    <  1:4605952/49‑1 (MQ=255)
                                    tctcTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTc                                                    <  1:532981/49‑1 (MQ=255)
                                      tctTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAg                                                  <  1:2367910/49‑1 (MQ=255)
                                        ttttGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAg                                                  >  1:1170137/1‑47 (MQ=255)
                                        ttttGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGa                             <  1:2528728/68‑1 (MQ=255)
                                          ttGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACa                           <  1:4651977/68‑1 (MQ=255)
                                          ttGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCt                        >  1:5557643/1‑71 (MQ=255)
                                            gCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCtt                       <  1:2004203/70‑1 (MQ=255)
                                             cTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTtc                      <  1:220242/70‑1 (MQ=255)
                                             cTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTtc                      <  1:4167243/70‑1 (MQ=255)
                                              ttCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAg                                                  <  1:2736064/41‑1 (MQ=255)
                                              ttCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAg                                                  <  1:4878457/41‑1 (MQ=255)
                                                cgcccATCGACACCCACACGTCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTtccgt                   >  1:3276132/3‑70 (MQ=255)
                                                 gcccATCGACACCCACACGTCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTtccgt                   <  1:5155262/68‑1 (MQ=255)
                                                 ccccATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTtcagtca                 <  1:1016743/71‑1 (MQ=255)
                                                 ccccATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTtcagt                   >  1:2729781/1‑69 (MQ=255)
                                                   ccATCGACACCCACACATCGGTATAGATAAAGTCAg                                                  <  1:4465292/36‑1 (MQ=255)
                                                   ccATCGACACCCACACATCGGTATAGATAAAGTCAg                                                  <  1:2073657/36‑1 (MQ=255)
                                                   ccATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTcg                                <  1:5627018/54‑1 (MQ=255)
                                                    cATCGACACCCACACGTCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTtccgtcagag              >  1:4499752/1‑69 (MQ=255)
                                                    cATCGACACCCACACGTCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTtccgtcagag              >  1:2895963/1‑69 (MQ=255)
                                                    cATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACAt                          >  1:4784833/1‑59 (MQ=255)
                                                    cATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACAt                          >  1:3795003/1‑59 (MQ=255)
                                                    cATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCg              <  1:771674/71‑1 (MQ=255)
                                                    cATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCg              <  1:1275353/71‑1 (MQ=255)
                                                     aTCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTcgcg                              >  1:5672435/1‑54 (MQ=255)
                                                     aTCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGt             <  1:1037820/71‑1 (MQ=255)
                                                     aTCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGt             <  1:4560752/71‑1 (MQ=255)
                                                     aTCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTACCTTcgcg                              >  1:1802820/1‑54 (MQ=255)
                                                      tCGACACCCACACGTCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTTCCGTCAGAGt             >  1:2500106/1‑70 (MQ=255)
                                                      tCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCg              <  1:1752024/69‑1 (MQ=255)
                                                      tCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGt             <  1:2239125/70‑1 (MQ=255)
                                                      tCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGt             <  1:5486764/70‑1 (MQ=255)
                                                      tCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTa            <  1:915455/71‑1 (MQ=255)
                                                        gACACCCACACGTCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTtcc                     <  1:273483/60‑2 (MQ=255)
                                                        gACACCCACACGTCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTtcc                     <  1:688040/60‑2 (MQ=255)
                                                        gACACCCACACGTCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTtcc                     <  1:1093968/60‑2 (MQ=255)
                                                        gACACCCACACGTCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTTCCGTCAGAGTaa           <  1:44697/70‑1 (MQ=255)
                                                        gACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa           <  1:5400366/70‑1 (MQ=255)
                                                        gACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa           >  1:201459/1‑70 (MQ=255)
                                                        gACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa           <  1:4719430/70‑1 (MQ=255)
                                                         acacCCACACGTCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTTCCGTCAGAGTAatt         <  1:136832/71‑2 (MQ=255)
                                                          cacCCACACGTCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTTCCGTCAGAGTAatt         <  1:4678501/70‑2 (MQ=255)
                                                          cacCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAatat        <  1:1667379/71‑1 (MQ=255)
                                                           acCCACACGTCGGTATAGATAAAGTCAGCGCCCTTAACGcct                                    >  1:5390758/1‑41 (MQ=19)
                                                             ccACACATCGGTATAGATAAAGTCAGCACCTTCAACt                                       <  1:1319840/37‑1 (MQ=255)
                                                             ccACACATCGGTATAGATAAAGTCAGCACCTTCAACTc                                      <  1:1558059/38‑1 (MQ=255)
                                                               acacgTCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTTCCGTCAGAGTAATTTTccc    <  1:502133/70‑1 (MQ=255)
                                                                cacgTCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTTCCGTCAGAGTAATTTTcccgc  <  1:4008764/71‑3 (MQ=255)
                                                                   |                                                                   
GATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCAC  >  minE/2918933‑2919067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A