Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,919,234 G→A 23.2% F154F (TTC→TTT)  argI ← ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,919,2340GA23.2% 233.6 / 65.1 125F154F (TTC→TTT) argIornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand):  ref base G (42/54);  new base A (17/12);  total (59/66)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

Only 100 of 129 total aligned reads displayed.

GCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAT  >  minE/2919166‑2919304
                                                                    |                                                                      
gcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGaa                                                                      <  1:4850436/71‑1 (MQ=255)
    cTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCt                                                                  <  1:3143561/71‑1 (MQ=255)
    cTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCt                                                                  <  1:3830421/71‑1 (MQ=255)
    cTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCt                                                                  <  1:4436257/71‑1 (MQ=255)
       ccaGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTg                                                               >  1:3993969/3‑71 (MQ=255)
       cGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTg                                                               >  1:659017/1‑71 (MQ=255)
       cGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTg                                                               >  1:3881427/1‑71 (MQ=255)
        gAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGc                                                              >  1:3463588/1‑71 (MQ=255)
        gAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGc                                                              >  1:4409404/1‑71 (MQ=255)
        gAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACAAGCGTCATTTCGTTGAACGATTTGc                                                              >  1:2598751/1‑71 (MQ=255)
         aGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGc                                                              <  1:3308479/70‑1 (MQ=255)
            aTCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCcgg                                                          <  1:4243788/71‑1 (MQ=255)
             tCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCcgg                                                          >  1:5584906/1‑70 (MQ=255)
             tCGAATTGCCCGTGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCggg                                                          >  1:4874228/1‑70 (MQ=255)
             tCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCttt                                                                <  1:1921360/64‑1 (MQ=255)
             tCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCttt                                                                <  1:921967/64‑1 (MQ=255)
               gagttGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCcgg                                                          >  1:2192853/4‑68 (MQ=255)
               gagttGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCcgg                                                          >  1:1837444/4‑68 (MQ=255)
               gagttGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCcgg                                                          >  1:4565363/4‑68 (MQ=255)
               gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGaa                                                                      >  1:4053313/1‑56 (MQ=255)
               gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGaa                                                                      >  1:4046558/1‑56 (MQ=255)
               gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGaa                                                                      >  1:2783816/1‑56 (MQ=255)
               gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa                                                        >  1:4089195/1‑70 (MQ=255)
               gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa                                                        <  1:4625081/70‑1 (MQ=255)
                aaTTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTg                                                                        <  1:1329905/53‑1 (MQ=255)
                 aTTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAAt                                                     >  1:1202170/1‑71 (MQ=255)
                 aTTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAAt                                                     >  1:3432057/1‑71 (MQ=255)
                   tGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGc                                                   >  1:663767/1‑71 (MQ=255)
                    gCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGc                                                   <  1:181489/70‑1 (MQ=255)
                    gCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGc                                                   <  1:3308099/70‑1 (MQ=255)
                    gCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGc                                                   <  1:3273038/70‑1 (MQ=255)
                    gCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCt                                                  >  1:5246231/1‑71 (MQ=255)
                     cccATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc                                                 <  1:5137429/71‑1 (MQ=255)
                       cATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCt                                               <  1:1725906/71‑1 (MQ=255)
                        aTGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCgg                                                           <  1:4587879/58‑1 (MQ=255)
                        aTGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCgg                                                           <  1:760622/58‑1 (MQ=255)
                         tgttgtTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGc                                                                   <  1:5447560/49‑1 (MQ=255)
                         tgttgtTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc                                                 <  1:5407096/67‑1 (MQ=255)
                         tgttgtTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGc                                             <  1:2808378/71‑1 (MQ=255)
                          gttgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGa                                                                       <  1:2684960/44‑1 (MQ=255)
                          gttgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGa                                                                       <  1:759527/44‑1 (MQ=255)
                           ttgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa                                                        <  1:4470762/58‑1 (MQ=255)
                           ttgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc                                                 >  1:1514884/1‑65 (MQ=255)
                           ttgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc                                                 >  1:4593094/1‑65 (MQ=255)
                           ttgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCa                                            <  1:5141844/70‑1 (MQ=255)
                            tgttGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATg                                          >  1:2632796/1‑71 (MQ=255)
                            tgttGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATg                                          >  1:3016110/1‑71 (MQ=255)
                              ttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATTGt                                        <  1:2013748/71‑1 (MQ=255)
                               tACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATg                                          >  1:4202588/1‑68 (MQ=255)
                               tACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGt                                        >  1:3598803/1‑70 (MQ=255)
                                gcgcgcaTCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGtca                                      >  1:650508/2‑69 (MQ=255)
                                 cgcgcaTCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGtca                                      <  1:846672/70‑3 (MQ=255)
                                      gTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTgag                                     >  1:5313960/1‑66 (MQ=255)
                                      gTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGAt                                <  1:4850782/71‑1 (MQ=255)
                                        cACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATcc                              >  1:2351368/1‑71 (MQ=255)
                                         aCCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCg                             >  1:1206329/1‑71 (MQ=255)
                                            tGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGc                            >  1:1455516/1‑69 (MQ=255)
                                              cATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCt                                                  <  1:4560080/45‑1 (MQ=255)
                                              cATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCt                                                  <  1:3144619/45‑1 (MQ=255)
                                               gtAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCcagca                       <  1:3256820/70‑1 (MQ=255)
                                               aTAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcagca                       <  1:2980714/71‑1 (MQ=255)
                                                tAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcagcag                      <  1:200232/71‑1 (MQ=255)
                                                  gACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCa                                            >  1:914893/1‑47 (MQ=255)
                                                  gACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCt                    <  1:2168190/71‑1 (MQ=255)
                                                  gACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCt                    <  1:5519083/71‑1 (MQ=255)
                                                  gACCAGCGCCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCt                    >  1:5551481/1‑71 (MQ=255)
                                                   aCCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTg                   >  1:4465203/1‑71 (MQ=255)
                                                   aCCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTg                   <  1:3663917/71‑1 (MQ=255)
                                                   aCCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTTCATGGTCATCAGGTCCGCCAGCAGCt                    <  1:79489/70‑1 (MQ=255)
                                                    ccAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcagcag                      >  1:3137687/1‑67 (MQ=255)
                                                    ccAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGc                  <  1:3548393/71‑1 (MQ=255)
                                                     cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCt                    >  1:3230000/1‑68 (MQ=255)
                                                     cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGc                  <  1:2206133/70‑1 (MQ=255)
                                                     cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCg                 >  1:3087521/1‑71 (MQ=255)
                                                     cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTACTGCATGGTGAGAAGATCCGCcagcag                      >  1:5283254/1‑66 (MQ=255)
                                                       gCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGtc               <  1:3738147/71‑2 (MQ=255)
                                                         gTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGt                                        >  1:3611139/1‑44 (MQ=16)
                                                         gTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTcgg             <  1:2875392/71‑1 (MQ=255)
                                                          tCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTcggg            <  1:988855/71‑2 (MQ=255)
                                                          tCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTcggg            >  1:958237/1‑70 (MQ=255)
                                                            aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGAt                                >  1:4524402/1‑49 (MQ=255)
                                                            aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGAt                                >  1:5114721/1‑49 (MQ=255)
                                                            aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGAt           >  1:2937142/1‑70 (MQ=255)
                                                            aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGAt           <  1:3775002/70‑1 (MQ=255)
                                                            aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGAt           >  1:1165251/1‑70 (MQ=255)
                                                             tttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTgag                                     <  1:3301538/43‑1 (MQ=255)
                                                             tttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATg          >  1:1521858/1‑70 (MQ=255)
                                                              ttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg         >  1:4612318/1‑70 (MQ=255)
                                                              ttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg         <  1:98710/70‑1 (MQ=255)
                                                              ttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg         >  1:2694170/1‑70 (MQ=255)
                                                               tCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTcggg            >  1:203654/1‑65 (MQ=255)
                                                               tCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGaa       >  1:4193708/1‑71 (MQ=255)
                                                                 gTTGTACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACt     <  1:5298801/71‑1 (MQ=255)
                                                                 gTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACt     <  1:2503699/71‑1 (MQ=255)
                                                                 gTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTgg         <  1:2222422/67‑1 (MQ=255)
                                                                   tGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcagc                        <  1:2175456/50‑1 (MQ=255)
                                                                   tGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg         <  1:5487741/65‑1 (MQ=255)
                                                                   tGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCa   >  1:94922/1‑71 (MQ=255)
                                                                    gAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAt  >  1:3541285/1‑71 (MQ=255)
                                                                    gAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAt  >  1:641879/1‑71 (MQ=255)
                                                                    |                                                                      
GCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAT  >  minE/2919166‑2919304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A