Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,919,246 G→C 24.0% P150P (CCC→CCG)  argI ← ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,919,2460GC24.0% 180.1 / 57.4 100P150P (CCC→CCG) argIornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand):  ref base G (34/42);  new base C (12/12);  total (46/54)
Fisher's exact test for biased strand distribution p-value = 8.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

ATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGC  >  minE/2919178‑2919313
                                                                    |                                                                   
aTCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCcgg                                                                   <  1:4243788/71‑1 (MQ=255)
aTCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCcgg                                                                   <  1:5507909/71‑1 (MQ=255)
 tCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCcgg                                                                   >  1:5584906/1‑70 (MQ=255)
 tCGAATTGCCCGTGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCggg                                                                   >  1:4874228/1‑70 (MQ=255)
   gagttGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCcgg                                                                   >  1:1837444/4‑68 (MQ=255)
   gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa                                                                 <  1:4625081/70‑1 (MQ=255)
   gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa                                                                 >  1:4089195/1‑70 (MQ=255)
     aTTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAAt                                                              >  1:1202170/1‑71 (MQ=255)
     aTTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAAt                                                              >  1:3432057/1‑71 (MQ=255)
       tGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGc                                                            >  1:663767/1‑71 (MQ=255)
        gCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGc                                                            <  1:3308099/70‑1 (MQ=255)
        gCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGc                                                            <  1:3273038/70‑1 (MQ=255)
        gCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCt                                                           >  1:5246231/1‑71 (MQ=255)
         cccATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc                                                          <  1:5137429/71‑1 (MQ=255)
           cATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCt                                                        <  1:1725906/71‑1 (MQ=255)
            aTGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCgg                                                                    <  1:4587879/58‑1 (MQ=255)
             tgttgtTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc                                                          <  1:4580676/67‑1 (MQ=255)
             tgttgtTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTg                                                       >  1:4613013/1‑70 (MQ=255)
             tgttgtTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGc                                                      <  1:2808378/71‑1 (MQ=255)
               ttgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa                                                                 <  1:4470762/58‑1 (MQ=255)
               ttgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc                                                          >  1:1514884/1‑65 (MQ=255)
               ttgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc                                                          >  1:4593094/1‑65 (MQ=255)
               ttgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCa                                                     <  1:5141844/70‑1 (MQ=255)
                tgttGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATg                                                   >  1:2632796/1‑71 (MQ=255)
                tgttGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATg                                                   >  1:911300/1‑71 (MQ=255)
                tgttGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATg                                                   >  1:3016110/1‑71 (MQ=255)
                tgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCgg                                                                    <  1:373920/54‑1 (MQ=255)
                tgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCgg                                                                    >  1:2894917/1‑54 (MQ=255)
                 gttGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCt                                                           >  1:647392/1‑62 (MQ=255)
                  ttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATTGt                                                 <  1:2013748/71‑1 (MQ=255)
                   tACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATg                                                   >  1:2044921/1‑68 (MQ=255)
                   tACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATg                                                   >  1:4202588/1‑68 (MQ=255)
                   tACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGt                                                 >  1:3598803/1‑70 (MQ=255)
                    gcgcgcaTCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGtca                                               >  1:650508/2‑69 (MQ=255)
                     cgcgcaTCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGtca                                               <  1:846672/70‑3 (MQ=255)
                       cgcaTCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGc                                                      <  1:4367059/61‑1 (MQ=255)
                          gTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTgag                                              >  1:5313960/1‑66 (MQ=255)
                          gTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGAt                                         <  1:4850782/71‑1 (MQ=255)
                            cACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTg                                                       <  1:3114670/55‑1 (MQ=255)
                            cACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATcc                                       >  1:2351368/1‑71 (MQ=255)
                             aCCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCg                                      >  1:1206329/1‑71 (MQ=255)
                                tGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGc                                     >  1:1455516/1‑69 (MQ=255)
                                 gCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGcc                                    <  1:5354942/69‑1 (MQ=255)
                                  cATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCt                                                           <  1:3144619/45‑1 (MQ=255)
                                  cATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCt                                                           <  1:4560080/45‑1 (MQ=255)
                                   gtAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCcagca                                <  1:3256820/70‑1 (MQ=255)
                                   aTAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcagca                                <  1:2980714/71‑1 (MQ=255)
                                    tAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcagcag                               <  1:200232/71‑1 (MQ=255)
                                      gACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCa                                                     >  1:914893/1‑47 (MQ=255)
                                      gACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCt                             <  1:2168190/71‑1 (MQ=255)
                                      gACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCt                             <  1:5519083/71‑1 (MQ=255)
                                      gACCAGCGCCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCt                             >  1:5551481/1‑71 (MQ=255)
                                       aCCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTg                            <  1:3663917/71‑1 (MQ=255)
                                       aCCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTg                            >  1:4465203/1‑71 (MQ=255)
                                       aCCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTTCATGGTCATCAGGTCCGCCAGCAGCt                             <  1:79489/70‑1 (MQ=255)
                                        ccAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcagcag                               >  1:3137687/1‑67 (MQ=255)
                                        ccAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGc                           <  1:3548393/71‑1 (MQ=255)
                                         cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGc                           <  1:2206133/70‑1 (MQ=255)
                                         cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCg                          >  1:3087521/1‑71 (MQ=255)
                                         cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCg                          <  1:2776115/71‑1 (MQ=255)
                                         cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTACTGCATGGTGAGAAGATCCGCcagcag                               >  1:5283254/1‑66 (MQ=255)
                                           gCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGtc                        <  1:3738147/71‑2 (MQ=255)
                                            cGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGtc                        <  1:1594569/70‑2 (MQ=255)
                                             gTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGt                                                 >  1:3611139/1‑44 (MQ=16)
                                              tCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTcggg                     >  1:958237/1‑70 (MQ=255)
                                              tCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTcggg                     <  1:988855/71‑2 (MQ=255)
                                                aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGAt                                         >  1:4524402/1‑49 (MQ=255)
                                                aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGAt                                         >  1:5114721/1‑49 (MQ=255)
                                                aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGAt                    >  1:2937142/1‑70 (MQ=255)
                                                aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGAt                    >  1:1165251/1‑70 (MQ=255)
                                                aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGAt                    <  1:3775002/70‑1 (MQ=255)
                                                 tttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTgag                                              <  1:3301538/43‑1 (MQ=255)
                                                 tttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTgag                                              <  1:1933938/43‑1 (MQ=255)
                                                 tttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATg                   >  1:1521858/1‑70 (MQ=255)
                                                  ttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg                  >  1:4612318/1‑70 (MQ=255)
                                                  ttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg                  <  1:98710/70‑1 (MQ=255)
                                                  ttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg                  >  1:2694170/1‑70 (MQ=255)
                                                   tCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTcggg                     >  1:1377731/1‑65 (MQ=255)
                                                   tCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTcggg                     >  1:203654/1‑65 (MQ=255)
                                                   tCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGaa                >  1:4193708/1‑71 (MQ=255)
                                                     gTTGTACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACt              <  1:5298801/71‑1 (MQ=255)
                                                     gTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACt              <  1:2503699/71‑1 (MQ=255)
                                                     gTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTgg                  <  1:2222422/67‑1 (MQ=255)
                                                       tGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcagc                                 <  1:2175456/50‑1 (MQ=255)
                                                       tGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg                  <  1:5487741/65‑1 (MQ=255)
                                                       tGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTcg            >  1:3286920/1‑70 (MQ=255)
                                                       tGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCa            >  1:94922/1‑71 (MQ=255)
                                                        gAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAt           >  1:641879/1‑71 (MQ=255)
                                                        gAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAt           >  1:3541285/1‑71 (MQ=255)
                                                        aaaCGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTgg                  <  1:2672590/63‑1 (MQ=255)
                                                        aaaCGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTcg            <  1:1719064/69‑2 (MQ=255)
                                                         aaCGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTcgt           <  1:893408/70‑3 (MQ=255)
                                                           cGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTgg        >  1:1824122/1‑71 (MQ=255)
                                                            gCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGt       <  1:3591007/71‑1 (MQ=255)
                                                             ccttGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACt              >  1:3075624/3‑63 (MQ=255)
                                                                 gCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCTAGCAGCTGCGTGGGATGGAACTCATTGGTCAgg   <  1:3382133/70‑1 (MQ=255)
                                                                 gCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAgg   <  1:5174129/70‑1 (MQ=255)
                                                                  ccGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACt              <  1:4193588/58‑1 (MQ=255)
                                                                  ccGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGc  <  1:4659488/70‑1 (MQ=255)
                                                                  ccGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGc  <  1:3721670/70‑1 (MQ=255)
                                                                    |                                                                   
ATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGC  >  minE/2919178‑2919313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A