Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,919,267 G→C 26.5% L143L (CTC→CTG) ‡ argI ← ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,919,2670GC26.5% 164.9 / 66.8 102L143L (CTC→CTG) ‡argIornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand):  ref base G (40/35);  new base C (13/14);  total (53/49)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 105 total aligned reads displayed.

GTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAG  >  minE/2919204‑2919334
                                                               |                                                                   
gTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTgag                                                                   >  1:5313960/1‑66 (MQ=255)
gTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGAt                                                              <  1:4850782/71‑1 (MQ=255)
  cACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATcc                                                            >  1:2351368/1‑71 (MQ=255)
   aCCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCg                                                           >  1:1206329/1‑71 (MQ=255)
      tGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGc                                                          >  1:1455516/1‑69 (MQ=255)
       gCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGcc                                                         <  1:5039536/69‑1 (MQ=255)
       gCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGcc                                                         <  1:5354942/69‑1 (MQ=255)
         gtAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCcagca                                                     <  1:3256820/70‑1 (MQ=255)
         aTAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcagca                                                     <  1:2980714/71‑1 (MQ=255)
          tAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcagcag                                                    <  1:200232/71‑1 (MQ=255)
            gACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCt                                                  <  1:5519083/71‑1 (MQ=255)
            gACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCt                                                  <  1:2168190/71‑1 (MQ=255)
            gACCAGCGCCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCt                                                  >  1:5551481/1‑71 (MQ=255)
             aCCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTg                                                 >  1:4465203/1‑71 (MQ=255)
             aCCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTg                                                 <  1:3663917/71‑1 (MQ=255)
             aCCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTTCATGGTCATCAGGTCCGCCAGCAGCt                                                  <  1:79489/70‑1 (MQ=255)
              ccAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcagcag                                                    >  1:3137687/1‑67 (MQ=255)
              ccAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGc                                                <  1:3548393/71‑1 (MQ=255)
               cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGc                                                <  1:2206133/70‑1 (MQ=255)
               cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCg                                               <  1:2776115/71‑1 (MQ=255)
               cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCg                                               >  1:3087521/1‑71 (MQ=255)
               cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTACTGCATGGTGAGAAGATCCGCcagcag                                                    >  1:5283254/1‑66 (MQ=255)
                 gCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGtc                                             <  1:3738147/71‑2 (MQ=255)
                  cGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGtc                                             <  1:1594569/70‑2 (MQ=255)
                   gTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTcgg                                           <  1:2875392/71‑1 (MQ=255)
                    tCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTcggg                                          >  1:958237/1‑70 (MQ=255)
                    tCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTcggg                                          <  1:988855/71‑2 (MQ=255)
                      aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGAt                                                              >  1:5114721/1‑49 (MQ=255)
                      aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGAt                                                              >  1:4524402/1‑49 (MQ=255)
                      aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGAt                                         <  1:3775002/70‑1 (MQ=255)
                      aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGAt                                         >  1:1165251/1‑70 (MQ=255)
                      aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGAt                                         >  1:2937142/1‑70 (MQ=255)
                       tttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTgag                                                                   <  1:1933938/43‑1 (MQ=255)
                       tttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTgag                                                                   <  1:3301538/43‑1 (MQ=255)
                       tttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATg                                        >  1:1521858/1‑70 (MQ=255)
                        ttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg                                       >  1:4612318/1‑70 (MQ=255)
                        ttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg                                       >  1:2694170/1‑70 (MQ=255)
                        ttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg                                       <  1:98710/70‑1 (MQ=255)
                         tCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTcggg                                          >  1:1377731/1‑65 (MQ=255)
                         tCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTcggg                                          >  1:203654/1‑65 (MQ=255)
                         tCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGaa                                     >  1:4193708/1‑71 (MQ=255)
                           gTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACt                                   <  1:2503699/71‑1 (MQ=255)
                           gTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTgg                                       <  1:2222422/67‑1 (MQ=255)
                            ttGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACt                                   <  1:4187329/70‑1 (MQ=255)
                             tGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg                                       <  1:5487741/65‑1 (MQ=255)
                             tGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTcg                                 >  1:3286920/1‑70 (MQ=255)
                             tGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCa                                 >  1:94922/1‑71 (MQ=255)
                              gAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAt                                >  1:3541285/1‑71 (MQ=255)
                              gAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAt                                >  1:641879/1‑71 (MQ=255)
                              aaaCGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTgg                                       <  1:2672590/63‑1 (MQ=255)
                              aaaCGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTcg                                 <  1:1719064/69‑2 (MQ=255)
                               aaCGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTcgt                                <  1:893408/70‑3 (MQ=255)
                                 cGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTgg                             >  1:1824122/1‑71 (MQ=255)
                                  gCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGt                            <  1:3591007/71‑1 (MQ=255)
                                   ccttGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACt                                   >  1:3075624/3‑63 (MQ=255)
                                       gCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCTAGCAGCTGCGTGGGATGGAACTCATTGGTCAgg                        <  1:3382133/70‑1 (MQ=255)
                                       gCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAgg                        <  1:5174129/70‑1 (MQ=255)
                                        ccGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACt                                   <  1:4193588/58‑1 (MQ=255)
                                        ccGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGc                       <  1:4659488/70‑1 (MQ=255)
                                        ccGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGc                       <  1:3721670/70‑1 (MQ=255)
                                            gCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTc                  <  1:1168233/71‑1 (MQ=255)
                                            gCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTc                  <  1:71432/71‑1 (MQ=255)
                                               aaTGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAt                                >  1:724246/1‑54 (MQ=255)
                                               aaTGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAt                                >  1:682287/1‑54 (MQ=255)
                                               aaTGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAt                                >  1:5669422/1‑54 (MQ=255)
                                               aaTGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCAt               <  1:2454188/71‑1 (MQ=255)
                                                gtGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTCGTTGGTCAGcccg                     <  1:1420698/63‑2 (MQ=255)
                                                 tGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCa                                 >  1:4413032/1‑51 (MQ=255)
                                                 tGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCa                                 >  1:3068557/1‑51 (MQ=255)
                                                 tGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCa                                 >  1:2230691/1‑51 (MQ=255)
                                                 tGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATAc             >  1:4737010/1‑71 (MQ=255)
                                                 tGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATAc             <  1:5521335/71‑1 (MQ=255)
                                                 tGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATAc             >  1:3730550/1‑71 (MQ=255)
                                                 tGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATAc             >  1:3789684/1‑71 (MQ=255)
                                                 tGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTCGTTGGTCAGCCCGTTCCacac             >  1:2477149/1‑71 (MQ=255)
                                                  gCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTCGTTGGTCAGCCCGTTCCa                >  1:3222573/1‑67 (MQ=255)
                                                   cTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAtt                               >  1:1986079/1‑51 (MQ=255)
                                                   cTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAtt                               >  1:2391817/1‑51 (MQ=255)
                                                   cTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAtt                               >  1:5339647/1‑51 (MQ=255)
                                                   cTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTCGTTGGTCa                          <  1:3334773/56‑1 (MQ=255)
                                                    tCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCgg          >  1:1028938/1‑71 (MQ=255)
                                                     ccTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTCGTTGGTCAGCCCGTTCCACACCgg          >  1:4908013/1‑70 (MQ=255)
                                                      cTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCAt               <  1:3892490/64‑1 (MQ=255)
                                                      cTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCa        >  1:1223372/1‑71 (MQ=255)
                                                      cTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCa        >  1:5638374/1‑71 (MQ=255)
                                                      cTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCa        >  1:4666714/1‑71 (MQ=255)
                                                      cTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCa        >  1:4673446/1‑71 (MQ=255)
                                                      cTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTcgt                                <  1:4455188/47‑3 (MQ=12)
                                                      cTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTCGTTGGTCAGCACGTTCCACACCGGCa        >  1:5084020/1‑71 (MQ=255)
                                                       tGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTCGTTGGTCAGCCCGTTCCac               <  1:5158794/63‑2 (MQ=255)
                                                         cATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGc     >  1:1811018/1‑71 (MQ=255)
                                                         cATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTCGTTGGTCAGCCCGTTCCACACCGGCACGc     >  1:666396/1‑71 (MQ=255)
                                                         cATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTCGTTGGTCAGCCCGTTCCACACCGGCACGc     >  1:3624200/1‑71 (MQ=255)
                                                          aTGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGc     >  1:4712972/1‑70 (MQ=255)
                                                          aTGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCt    <  1:1884845/71‑1 (MQ=255)
                                                            ggTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGt                            <  1:4166004/45‑1 (MQ=255)
                                                            ggTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAg  >  1:4734366/1‑71 (MQ=255)
                                                             gTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGc                       <  1:2185865/49‑1 (MQ=255)
                                                             gTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCt    <  1:5235145/68‑1 (MQ=255)
                                                              tGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCg           <  1:1008725/60‑1 (MQ=255)
                                                               |                                                                   
GTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAG  >  minE/2919204‑2919334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A