Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,919,369 A→G 21.0% Y109Y (TAT→TAC) ‡ argI ← ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,919,3690AG21.0% 264.2 / 66.6 138Y109Y (TAT→TAC) ‡argIornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand):  ref base A (62/47);  new base G (18/11);  total (80/58)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

Only 100 of 141 total aligned reads displayed.

ACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTAT  >  minE/2919299‑2919439
                                                                      |                                                                      
 cTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCAt                                                                       <  1:2992503/71‑1 (MQ=255)
 cTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCAt                                                                       <  1:3328650/71‑1 (MQ=255)
 cTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCAt                                                                       <  1:1589782/71‑1 (MQ=255)
   cATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAg                                                                     >  1:1217205/1‑71 (MQ=255)
    aTTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGc                                                                    >  1:5324878/1‑71 (MQ=255)
    aTTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGc                                                                    >  1:5674693/1‑71 (MQ=255)
        gTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCcgc                                                                 <  1:2879803/70‑1 (MQ=255)
        gTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCcgc                                                                 <  1:4780463/70‑1 (MQ=255)
        gTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCcgc                                                                 <  1:571441/70‑1 (MQ=255)
         tCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGa                                                               <  1:1858072/71‑1 (MQ=255)
         tCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGa                                                               <  1:3589598/71‑1 (MQ=255)
           aGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATa                                                             >  1:2758018/1‑71 (MQ=255)
            ggCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATa                                                                      <  1:4341432/61‑1 (MQ=255)
            ggCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGa                                                               <  1:241723/68‑1 (MQ=255)
              ccgttCCACACCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACt                                                           >  1:2042270/4‑70 (MQ=255)
              ccgttCCACACCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACt                                                           >  1:306238/4‑70 (MQ=255)
              ccATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTg                                                          <  1:1675769/71‑1 (MQ=255)
               cgttCCACACCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGa                                                         <  1:475425/69‑1 (MQ=255)
               cATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGa                                                         <  1:4742506/71‑1 (MQ=255)
                gttCCACACCGGCACGCCCGCATACTGCGCCAGCGTATCGACCACTTCCTGGCCGTGACCGCGATACTg                                                          <  1:1508771/68‑1 (MQ=255)
                   ccATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATAc                                                     >  1:3929235/1‑71 (MQ=255)
                   ccATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATAc                                                     >  1:5014182/1‑71 (MQ=255)
                   ccACACCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAAtgc                                                     >  1:3211553/1‑69 (MQ=255)
                     aTACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCcgcg                                                                <  1:4121044/58‑1 (MQ=255)
                      tACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGa                                                               <  1:4624778/58‑1 (MQ=255)
                      tACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGt                                                  <  1:4988659/71‑1 (MQ=255)
                       aCCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGa                                                               >  1:4976226/1‑57 (MQ=255)
                       aCCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGt                                                  >  1:1745219/1‑70 (MQ=255)
                       aCCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTc                                                 <  1:4298337/71‑1 (MQ=255)
                       aCCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTc                                                 <  1:333869/71‑1 (MQ=255)
                        ccGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTc                                                 <  1:3940701/70‑1 (MQ=255)
                        ccGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCa                                                >  1:4518782/1‑71 (MQ=255)
                        ccGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCa                                                >  1:2263157/1‑71 (MQ=255)
                          ggCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATa                                              <  1:2234140/71‑1 (MQ=255)
                          ggCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATa                                              >  1:3176107/1‑71 (MQ=255)
                           gCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATa                                              >  1:484841/1‑70 (MQ=255)
                           gCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATa                                              >  1:269145/1‑70 (MQ=255)
                            cACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACa                                            >  1:10161/1‑71 (MQ=255)
                               gCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATa                                                             >  1:3112073/1‑51 (MQ=255)
                               gCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGc                                         <  1:3089849/71‑1 (MQ=255)
                                cTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGc                                         >  1:400834/1‑70 (MQ=255)
                                cTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCg                                        <  1:4496304/71‑1 (MQ=255)
                                   gCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGa                                       <  1:5155006/69‑1 (MQ=255)
                                     gTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGAcc                                     >  1:4983560/1‑69 (MQ=255)
                                      tATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAg                                  >  1:2112056/1‑71 (MQ=255)
                                      tATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATAATGAATACCGTCATACATGCGACCAAg                                  >  1:5192670/1‑71 (MQ=255)
                                       aTTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAg                                  >  1:1579204/1‑70 (MQ=255)
                                       aTTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAg                                  >  1:776690/1‑70 (MQ=255)
                                        ttCCGCCAGTGTTTCGACAATCTCCTGACCATAGcc                                                                   >  1:4921986/1‑36 (MQ=255)
                                        ttCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGaa                                                        >  1:1573367/1‑47 (MQ=255)
                                        ttCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCa                                                >  1:2796713/1‑55 (MQ=255)
                                        ttCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCa                                                >  1:1888607/1‑55 (MQ=255)
                                         tCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCa                                >  1:5674224/1‑70 (MQ=255)
                                          ccGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAcg                              >  1:832260/1‑71 (MQ=255)
                                          ccGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAc                               <  1:74845/70‑1 (MQ=255)
                                          ccGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAc                               <  1:949554/70‑1 (MQ=255)
                                           cGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACa                                            >  1:4034677/1‑56 (MQ=255)
                                           cGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACa                                            >  1:975375/1‑56 (MQ=255)
                                            gCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGt                                                  <  1:2428923/49‑1 (MQ=255)
                                              cAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCa                                                >  1:1321866/1‑49 (MQ=255)
                                              cAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCa                                                >  1:550620/1‑49 (MQ=255)
                                              cAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCggg                          <  1:4073582/71‑1 (MQ=255)
                                              cAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAAcccgcg                          <  1:4149179/71‑3 (MQ=255)
                                               agcgtTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCgcgc                         <  1:1399009/68‑1 (MQ=255)
                                               agcgtTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCgcgc                         <  1:2994542/68‑1 (MQ=255)
                                                gtgtTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCggg                          >  1:1392164/1‑69 (MQ=255)
                                                gtgtTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCggg                          >  1:846323/1‑69 (MQ=255)
                                                gtgtTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCggg                          >  1:1101333/1‑69 (MQ=255)
                                                gtgtTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCggg                          >  1:3615736/1‑69 (MQ=255)
                                                 cgtTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCgcgcgg                       >  1:1599345/2‑69 (MQ=255)
                                                  gtTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACAtc                                          >  1:1706309/1‑50 (MQ=255)
                                                  gtTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACAtc                                          >  1:4166330/1‑50 (MQ=255)
                                                  gtTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAgaa                                >  1:2348327/1‑59 (MQ=255)
                                                   tttCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGa                                       <  1:1687128/53‑1 (MQ=255)
                                                   tttCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGa                                       <  1:4010447/53‑1 (MQ=255)
                                                   tttCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGa                                       <  1:3460941/53‑1 (MQ=255)
                                                   tttCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAgtg                     >  1:261763/1‑71 (MQ=255)
                                                   tttCGACAATCTCCTGACAATAGCCGCGATACTGAATACCGTCATAc                                             <  1:1045940/47‑1 (MQ=255)
                                                     tCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCaa                                   >  1:4513022/1‑55 (MQ=255)
                                                     tCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCa                                >  1:137224/1‑58 (MQ=255)
                                                     tCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTc                   >  1:4688723/1‑71 (MQ=255)
                                                      cGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCa                                >  1:3830768/1‑57 (MQ=255)
                                                       gACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCa                        >  1:4847672/1‑64 (MQ=255)
                                                       gACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCtt                 <  1:5474177/71‑1 (MQ=255)
                                                       gACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCtt                 <  1:2301822/71‑1 (MQ=255)
                                                         cAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCa                                >  1:5171538/1‑54 (MQ=255)
                                                          aaTCTCCTGACCATAGCCGCGGTACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTaa              >  1:1903331/1‑71 (MQ=255)
                                                          aaTCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAcgc                             >  1:2663031/1‑56 (MQ=255)
                                                          aaTCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTaa              <  1:1606965/71‑1 (MQ=255)
                                                                cTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATc            <  1:1424075/67‑1 (MQ=255)
                                                                cTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGa          >  1:2821532/1‑69 (MQ=255)
                                                                cTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGAct        >  1:2343272/1‑71 (MQ=255)
                                                                cTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGAGTCTTTAATCGAct        >  1:772278/1‑71 (MQ=255)
                                                                 tGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGa          <  1:629304/68‑1 (MQ=255)
                                                                 tGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGActc       >  1:5559379/1‑71 (MQ=255)
                                                                  gACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTAAATCGActct      >  1:2658552/1‑71 (MQ=255)
                                                                   aCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCa                                >  1:1658580/1‑44 (MQ=255)
                                                                   aCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAgtgt                    >  1:2735544/1‑56 (MQ=255)
                                                                    ccATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGa          >  1:2899789/1‑65 (MQ=255)
                                                                      aTAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTAt  >  1:612447/1‑71 (MQ=255)
                                                                      |                                                                      
ACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTAT  >  minE/2919299‑2919439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A