Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,919,459 T→C 26.4% P79P (CCA→CCG)  argI ← ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,919,4590TC26.4% 217.1 / 90.8 140P79P (CCA→CCG) argIornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand):  ref base T (55/48);  new base C (30/7);  total (85/55)
Fisher's exact test for biased strand distribution p-value = 3.21e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01

Only 100 of 143 total aligned reads displayed.

CGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGT  >  minE/2919389‑2919527
                                                                      |                                                                    
cGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCtt                                                                      <  1:1396736/71‑1 (MQ=255)
 gTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTg                                                                     <  1:94395/71‑1 (MQ=255)
 gTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTg                                                                     >  1:4752809/1‑71 (MQ=255)
 gTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTg                                                                     >  1:1869083/1‑71 (MQ=255)
 gTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTg                                                                     >  1:5531244/1‑71 (MQ=255)
    aTACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGc                                                                  >  1:3595857/1‑71 (MQ=255)
    aTACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCggcc                                                                  >  1:3962020/1‑69 (MQ=255)
     tACATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGccc                                                                 >  1:1830407/1‑71 (MQ=255)
      aCATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGcc                                                                 >  1:4259163/1‑70 (MQ=255)
      aCATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGccc                                                                 >  1:5546291/1‑70 (MQ=255)
      aCATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGccc                                                                 >  1:4988122/1‑70 (MQ=255)
       cATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGcc                                                                 >  1:5194333/1‑69 (MQ=255)
       cATGCGACCAACCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCga                                                               <  1:2594551/71‑1 (MQ=255)
       cATCCGCCCGAGAACCCGCGCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGcccta                                                               <  1:2485555/71‑3 (MQ=255)
         tGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGcc                                                                 >  1:3635020/1‑67 (MQ=255)
         tGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGcc                                                                 >  1:3080077/1‑67 (MQ=255)
          gCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCgaga                                                             >  1:4028116/1‑70 (MQ=255)
              ccAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGt                                                        <  1:22761/71‑1 (MQ=255)
               cAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGt                                                        <  1:867675/70‑1 (MQ=255)
                aaGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTaa                                                      >  1:3226887/1‑71 (MQ=255)
                 aGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCtt                                                                      <  1:2231518/54‑1 (MQ=255)
                 aGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTaa                                                      <  1:4695923/70‑1 (MQ=255)
                   cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGcc                                                                 <  1:4417167/57‑1 (MQ=255)
                   cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                   <  1:1254243/71‑1 (MQ=255)
                   cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                   >  1:3650320/1‑71 (MQ=255)
                   cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                   <  1:4518990/71‑1 (MQ=255)
                    aCGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                   <  1:5614383/70‑1 (MQ=255)
                            aGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCt                                          <  1:4949510/71‑1 (MQ=255)
                              tgtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAcc                                            <  1:5352630/67‑1 (MQ=255)
                                tccttAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCATAAATAGGTACCGCGCGCGCCCTGGt                                       >  1:5591149/4‑70 (MQ=255)
                                tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATa                                                           >  1:450414/1‑50 (MQ=255)
                                tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCa                                              <  1:4228580/63‑1 (MQ=255)
                                  tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATa                                                           >  1:40336/1‑48 (MQ=255)
                                  tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTatc                                                     >  1:4138938/1‑52 (MQ=255)
                                  tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                     >  1:5383000/1‑70 (MQ=255)
                                    tAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCaaat                                  >  1:1306106/1‑71 (MQ=255)
                                    tAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCaaat                                  >  1:5364924/1‑71 (MQ=255)
                                    tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCatat                                  >  1:4699447/1‑71 (MQ=255)
                                    tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                     <  1:4431115/68‑1 (MQ=255)
                                     aaTCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa                                                 >  1:1250657/1‑55 (MQ=255)
                                      aTCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGt                                       >  1:5045680/1‑64 (MQ=255)
                                       ttgACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCg                               >  1:4575185/3‑71 (MQ=255)
                                       tCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                     <  1:2831777/65‑1 (MQ=255)
                                         gACTCTTTATGCCCAATCTGGCTTCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCgg                              >  1:4404883/1‑70 (MQ=255)
                                         gACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGccc                                           >  1:4104350/1‑57 (MQ=255)
                                         gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGGGAGCACCCTGGTCATATGCGGc                             <  1:1807272/71‑1 (MQ=255)
                                         gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCgg                              >  1:3092168/1‑70 (MQ=255)
                                         gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCgg                              >  1:2110440/1‑70 (MQ=255)
                                         gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGc                             <  1:218339/71‑1 (MQ=255)
                                         gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGc                             <  1:2767777/71‑1 (MQ=255)
                                          aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAccc                                           <  1:136070/56‑1 (MQ=255)
                                          aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAccc                                           <  1:3902671/56‑1 (MQ=255)
                                          aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAccc                                           <  1:2929451/56‑1 (MQ=255)
                                          aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCa                            <  1:3390358/71‑1 (MQ=255)
                                           ctctTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAAcgcg                                                  <  1:631830/48‑1 (MQ=19)
                                            tctTTATGACCAATCTGGCTGCCGCTTGGGCCgag                                                              <  1:941693/35‑1 (MQ=255)
                                            tctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAg                                                         <  1:3961688/40‑1 (MQ=255)
                                             ctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa                                                 >  1:45659/1‑47 (MQ=255)
                                              tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAg                                                >  1:867150/1‑47 (MQ=255)
                                              tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCata                                   >  1:2714018/1‑60 (MQ=255)
                                              tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACt                         >  1:1653631/1‑70 (MQ=255)
                                               ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAcc                                            >  1:3580523/1‑50 (MQ=255)
                                               ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                       >  1:2903921/1‑71 (MQ=255)
                                                tATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTc                       >  1:3443826/1‑70 (MQ=255)
                                                tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGt                                                        >  1:576103/1‑37 (MQ=255)
                                                tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCt                                          >  1:145437/1‑51 (MQ=255)
                                                tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCaa                           >  1:5332485/1‑66 (MQ=255)
                                                tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                       <  1:694644/70‑1 (MQ=255)
                                                  tgcccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGa                     <  1:3845007/67‑1 (MQ=255)
                                                  tgcccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGa                     <  1:280392/67‑1 (MQ=255)
                                                  tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa                     <  1:3818374/70‑1 (MQ=255)
                                                   gACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa                     <  1:3811750/69‑1 (MQ=255)
                                                    aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                       <  1:2384455/66‑1 (MQ=255)
                                                    aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                       <  1:4730925/66‑1 (MQ=255)
                                                    aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGACCACCCTGGTCATATGCGGCAACTTCGa                     >  1:3750491/1‑68 (MQ=255)
                                                      cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaaa                   >  1:5187240/1‑68 (MQ=255)
                                                      cAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCaa                           >  1:2786265/1‑60 (MQ=255)
                                                       aaTCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAagag                >  1:4736921/1‑70 (MQ=255)
                                                        aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCa              >  1:3479276/1‑71 (MQ=255)
                                                          cTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCg                               >  1:1385077/1‑52 (MQ=255)
                                                          cTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCg                               >  1:5238320/1‑52 (MQ=255)
                                                          cTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCaac            >  1:5124203/1‑69 (MQ=255)
                                                           tGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCg           >  1:1763425/1‑71 (MQ=255)
                                                           tGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCaa                           >  1:1343995/1‑55 (MQ=255)
                                                           tGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACg           >  1:302305/1‑71 (MQ=255)
                                                             tcTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGGCAAATGCGGCAACTTCGAAAGAGCaac            <  1:227589/67‑3 (MQ=255)
                                                             gCTGCCGCTTGTGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCggg         <  1:2123353/71‑1 (MQ=255)
                                                             gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATc            <  1:2610503/68‑1 (MQ=255)
                                                             gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCgg          <  1:3465091/70‑1 (MQ=255)
                                                             gCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACggg         >  1:726144/1‑71 (MQ=255)
                                                              cTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACggg         >  1:3978607/1‑70 (MQ=255)
                                                              cTGCAGCTTGGGCCGAGATAAGTAACGCGAGCACCCTgg                                        >  1:225472/1‑39 (MQ=255)
                                                                gCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTa       >  1:3230002/1‑70 (MQ=255)
                                                                gCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAc      >  1:4940451/1‑71 (MQ=255)
                                                                 ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                     <  1:1307294/39‑1 (MQ=255)
                                                                 ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                     <  1:1003907/39‑1 (MQ=255)
                                                                  cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTa       >  1:751527/1‑68 (MQ=255)
                                                                  cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTa       >  1:1963677/1‑68 (MQ=255)
                                                                  cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga    <  1:1587703/71‑1 (MQ=255)
                                                                    cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagt  >  1:342421/1‑71 (MQ=255)
                                                                      |                                                                    
CGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGT  >  minE/2919389‑2919527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A