Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,919,465 G→T 23.0% L77L (CTC→CTA) ‡ argI ← ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,919,4650GT23.0% 283.6 / 59.5 126L77L (CTC→CTA) ‡argIornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand):  ref base G (44/53);  new base T (24/5);  total (68/58)
Fisher's exact test for biased strand distribution p-value = 5.28e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 129 total aligned reads displayed.

CATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCT  >  minE/2919396‑2919534
                                                                     |                                                                     
cATGCGACCAACCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCga                                                                      <  1:2594551/71‑1 (MQ=255)
   gCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCgaga                                                                    >  1:4028116/1‑70 (MQ=255)
       ccAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGt                                                               <  1:22761/71‑1 (MQ=255)
        cAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGt                                                               <  1:867675/70‑1 (MQ=255)
        cAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTa                                                              <  1:4491596/71‑1 (MQ=255)
         aaGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTaa                                                             >  1:3226887/1‑71 (MQ=255)
          aGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTaa                                                             <  1:4695923/70‑1 (MQ=255)
           gCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATaa                                                                 >  1:5623959/1‑65 (MQ=255)
            cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGt                                                               >  1:2871870/1‑66 (MQ=255)
            cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                          >  1:3650320/1‑71 (MQ=255)
            cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                          <  1:4518990/71‑1 (MQ=255)
            cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                          <  1:1254243/71‑1 (MQ=255)
                    cggtgtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGccc                                                  <  1:1362944/69‑1 (MQ=255)
                      gtgtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTg                                                >  1:3233839/1‑71 (MQ=255)
                      gtgtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTg                                                >  1:2540900/1‑71 (MQ=255)
                       tgtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAcc                                                   <  1:5352630/67‑1 (MQ=255)
                         tccttAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCATAAATAGGTACCGCGCGCGCCCTGGt                                              >  1:5591149/4‑70 (MQ=255)
                         tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCa                                                     <  1:4228580/63‑1 (MQ=255)
                           tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTatc                                                            >  1:4138938/1‑52 (MQ=255)
                           tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgcg                                                         <  1:1259835/57‑1 (MQ=255)
                           tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAc                                                            >  1:3718214/1‑54 (MQ=255)
                           tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa                                                        >  1:523689/1‑58 (MQ=255)
                           tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                            >  1:5383000/1‑70 (MQ=255)
                           cttAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCa                                            >  1:228556/2‑70 (MQ=255)
                             tAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCaaat                                         >  1:1306106/1‑71 (MQ=255)
                             tAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCaaat                                         >  1:5364924/1‑71 (MQ=255)
                             tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                          <  1:2132330/54‑1 (MQ=255)
                             tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCatat                                         >  1:4699447/1‑71 (MQ=255)
                             tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                            <  1:3904649/68‑1 (MQ=255)
                             tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                            <  1:4431115/68‑1 (MQ=255)
                              aaTCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa                                                        >  1:1250657/1‑55 (MQ=255)
                               aTCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGt                                              >  1:5045680/1‑64 (MQ=255)
                                ttgACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCg                                      >  1:1819107/3‑71 (MQ=255)
                                tCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                            <  1:2831777/65‑1 (MQ=255)
                                  gACTCTTTATGCCCAATCTGGCTTCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCgg                                     >  1:4404883/1‑70 (MQ=255)
                                  gACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGccc                                                  >  1:4104350/1‑57 (MQ=255)
                                  gACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCg                                      >  1:717621/1‑69 (MQ=255)
                                  gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGGGAGCACCCTGGTCATATGCGGc                                    <  1:1807272/71‑1 (MQ=255)
                                  gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCgg                                     >  1:2110440/1‑70 (MQ=255)
                                  gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGc                                    <  1:2767777/71‑1 (MQ=255)
                                   aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAccc                                                  <  1:136070/56‑1 (MQ=255)
                                   aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAccc                                                  <  1:3902671/56‑1 (MQ=255)
                                   aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCa                                   <  1:3390358/71‑1 (MQ=255)
                                    ctctTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAAcgcg                                                         <  1:631830/48‑1 (MQ=19)
                                     tctTTATGACCAATCTGGCTGCCGCTTGGGCCgag                                                                     <  1:941693/35‑1 (MQ=255)
                                      ctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa                                                        >  1:45659/1‑47 (MQ=255)
                                       tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAg                                                       >  1:867150/1‑47 (MQ=255)
                                       tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCata                                          >  1:2714018/1‑60 (MQ=255)
                                       tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACt                                >  1:1653631/1‑70 (MQ=255)
                                        ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAcc                                                   >  1:3580523/1‑50 (MQ=255)
                                        ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                              >  1:153013/1‑71 (MQ=255)
                                        ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                              >  1:2903921/1‑71 (MQ=255)
                                         tATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTc                              >  1:3443826/1‑70 (MQ=255)
                                         tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGt                                                               >  1:576103/1‑37 (MQ=255)
                                         tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTaa                                                             >  1:2803130/1‑39 (MQ=255)
                                         tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCt                                                 >  1:145437/1‑51 (MQ=255)
                                         tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                              >  1:3174424/1‑70 (MQ=255)
                                         tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                              <  1:694644/70‑1 (MQ=255)
                                           tgcccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGa                            <  1:3845007/67‑1 (MQ=255)
                                           tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa                            <  1:513397/70‑1 (MQ=255)
                                           tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa                            <  1:3818374/70‑1 (MQ=255)
                                           tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa                            <  1:2969478/70‑1 (MQ=255)
                                             aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                              <  1:4730925/66‑1 (MQ=255)
                                             aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGACCACCCTGGTCATATGCGGCAACTTCGa                            >  1:3750491/1‑68 (MQ=255)
                                              ccAATCTGGCTGCCGCTTGGGGCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaaa                          >  1:4659433/1‑69 (MQ=255)
                                               cAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCggaa                                   >  1:4806502/1‑57 (MQ=255)
                                               cAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCaa                                  >  1:2786265/1‑60 (MQ=255)
                                                aaTCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAagag                       >  1:4736921/1‑70 (MQ=255)
                                                 aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCa                                                     >  1:5562645/1‑39 (MQ=255)
                                                 aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCa                     >  1:3479276/1‑71 (MQ=255)
                                                 aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCa                     >  1:397055/1‑71 (MQ=255)
                                                   cTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCg                                      >  1:1385077/1‑52 (MQ=255)
                                                   cTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCg                                      >  1:5238320/1‑52 (MQ=255)
                                                   cTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGaaa                          >  1:495666/1‑64 (MQ=255)
                                                   cTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCaac                   >  1:5124203/1‑69 (MQ=255)
                                                    tGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCg                  >  1:1763425/1‑71 (MQ=255)
                                                    tGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACg                  >  1:302305/1‑71 (MQ=255)
                                                      tcTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGGCAAATGCGGCAACTTCGAAAGAGCaac                   <  1:227589/67‑3 (MQ=255)
                                                      gCTGCCGCTTGTGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCggg                <  1:2123353/71‑1 (MQ=255)
                                                      gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATc                   <  1:2610503/68‑1 (MQ=255)
                                                      gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCgg                 <  1:4551957/70‑1 (MQ=255)
                                                      gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCgg                 <  1:3465091/70‑1 (MQ=255)
                                                      gCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACggg                >  1:5339171/1‑71 (MQ=255)
                                                      gCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACggg                >  1:726144/1‑71 (MQ=255)
                                                       cTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAaga                        >  1:4036508/1‑62 (MQ=255)
                                                       cTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACggg                >  1:3978607/1‑70 (MQ=255)
                                                       cTGCAGCTTGGGCCGAGATAAGTAACGCGAGCACCCTgg                                               >  1:225472/1‑39 (MQ=255)
                                                         gCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTa              >  1:3230002/1‑70 (MQ=255)
                                                         gCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAc             >  1:4940451/1‑71 (MQ=255)
                                                          ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                            <  1:1307294/39‑1 (MQ=255)
                                                          ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                            <  1:1003907/39‑1 (MQ=255)
                                                           cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTa              >  1:1963677/1‑68 (MQ=255)
                                                           cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga           <  1:1587703/71‑1 (MQ=255)
                                                             cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagt         >  1:1326477/1‑71 (MQ=255)
                                                             cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagt         >  1:342421/1‑71 (MQ=255)
                                                                gggCCGAGTTAAGTAACGCGAGCACCCTGGTCATATGCCGCAACTTCGAAAGAGCATCGGGTAcgagtcg       <  1:5222055/70‑1 (MQ=255)
                                                                 ggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc        <  1:3089739/68‑1 (MQ=255)
                                                                  gCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa                            <  1:684815/47‑1 (MQ=255)
                                                                    cGAGATAAGTAACGCGAGCACCGTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCt  <  1:3426440/71‑1 (MQ=255)
                                                                    cGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa                            <  1:5400427/45‑1 (MQ=255)
                                                                     |                                                                     
CATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCT  >  minE/2919396‑2919534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A