Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,919,471 A→G 27.0% T75T (ACT→ACC)  argI ← ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,919,4710AG27.0% 209.4 / 89.8 137T75T (ACT→ACC) argIornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand):  ref base A (43/57);  new base G (28/9);  total (71/66)
Fisher's exact test for biased strand distribution p-value = 9.31e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 142 total aligned reads displayed.

CCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCG  >  minE/2919403‑2919540
                                                                    |                                                                     
ccAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGt                                                                     <  1:22761/71‑1 (MQ=255)
 cAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGt                                                                     <  1:867675/70‑1 (MQ=255)
 cAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTa                                                                    <  1:4491596/71‑1 (MQ=255)
 cAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTa                                                                    <  1:3437969/71‑1 (MQ=255)
  aaGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTaa                                                                   >  1:3226887/1‑71 (MQ=255)
   aGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTaa                                                                   <  1:4695923/70‑1 (MQ=255)
    gCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATaa                                                                       >  1:5623959/1‑65 (MQ=255)
     cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                                >  1:3650320/1‑71 (MQ=255)
     cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                                <  1:1254243/71‑1 (MQ=255)
      aCGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                                <  1:5614383/70‑1 (MQ=255)
              aGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCt                                                       <  1:4949510/71‑1 (MQ=255)
               gtgtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTg                                                      >  1:3233839/1‑71 (MQ=255)
                tgtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAcc                                                         <  1:5352630/67‑1 (MQ=255)
                  tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCa                                                           <  1:4228580/63‑1 (MQ=255)
                    tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAc                                                                  >  1:3718214/1‑54 (MQ=255)
                    tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa                                                              >  1:523689/1‑58 (MQ=255)
                      tAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCaaat                                               >  1:1306106/1‑71 (MQ=255)
                      tAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCaaat                                               >  1:5364924/1‑71 (MQ=255)
                      tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc                                                                <  1:2132330/54‑1 (MQ=255)
                      tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                                  <  1:4431115/68‑1 (MQ=255)
                      tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                                  <  1:3904649/68‑1 (MQ=255)
                       aaTCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa                                                              >  1:1250657/1‑55 (MQ=255)
                        aTCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGt                                                    >  1:5045680/1‑64 (MQ=255)
                         ttgACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCg                                            >  1:4575185/3‑71 (MQ=255)
                         tCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                                  <  1:2831777/65‑1 (MQ=255)
                           gACTCTTTATGCCCAATCTGGCTTCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCgg                                           >  1:4404883/1‑70 (MQ=255)
                           gACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGccc                                                        >  1:4104350/1‑57 (MQ=255)
                           gACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCg                                            >  1:717621/1‑69 (MQ=255)
                           gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGGGAGCACCCTGGTCATATGCGGc                                          <  1:1807272/71‑1 (MQ=255)
                           gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCgg                                           >  1:2110440/1‑70 (MQ=255)
                           gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGc                                          <  1:218339/71‑1 (MQ=255)
                           gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGc                                          <  1:2767777/71‑1 (MQ=255)
                            aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAccc                                                        <  1:136070/56‑1 (MQ=255)
                            aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAccc                                                        <  1:3902671/56‑1 (MQ=255)
                            aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCa                                         <  1:3390358/71‑1 (MQ=255)
                             ctctTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAAcgcg                                                               <  1:631830/48‑1 (MQ=19)
                              tctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAg                                                                      <  1:3961688/40‑1 (MQ=255)
                              tctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                                  <  1:2669303/60‑1 (MQ=255)
                               ctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa                                                              >  1:45659/1‑47 (MQ=255)
                                tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGc                                          <  1:3622142/66‑1 (MQ=255)
                                tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACt                                      >  1:1653631/1‑70 (MQ=255)
                                 ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                                    >  1:2903921/1‑71 (MQ=255)
                                  tATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTc                                    >  1:3443826/1‑70 (MQ=255)
                                  tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTaa                                                                   >  1:2803130/1‑39 (MQ=255)
                                  tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCt                                                       >  1:145437/1‑51 (MQ=255)
                                  tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCaa                                        >  1:5332485/1‑66 (MQ=255)
                                  tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                                    >  1:3174424/1‑70 (MQ=255)
                                  tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                                    <  1:694644/70‑1 (MQ=255)
                                    tgcccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGa                                  <  1:3845007/67‑1 (MQ=255)
                                    tgcccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGa                                  <  1:280392/67‑1 (MQ=255)
                                    tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa                                  <  1:2969478/70‑1 (MQ=255)
                                     gACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa                                  <  1:207597/69‑1 (MQ=255)
                                     gACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa                                  <  1:3811750/69‑1 (MQ=255)
                                      aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                                    <  1:2384455/66‑1 (MQ=255)
                                      aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc                                    <  1:4730925/66‑1 (MQ=255)
                                       ccAATCTGGCTGCCGCTTGGGGCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaaa                                >  1:4659433/1‑69 (MQ=255)
                                        cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaaa                                >  1:5187240/1‑68 (MQ=255)
                                        cAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCggaa                                         >  1:4806502/1‑57 (MQ=255)
                                        cAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAagag                             >  1:4733164/1‑71 (MQ=255)
                                          aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCa                                                           >  1:5562645/1‑39 (MQ=255)
                                          aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCa                           >  1:397055/1‑71 (MQ=255)
                                          aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCa                           >  1:3479276/1‑71 (MQ=255)
                                           tCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaaa                                <  1:5384988/65‑1 (MQ=255)
                                            cTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCg                                            >  1:5238320/1‑52 (MQ=255)
                                            cTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCg                                            >  1:1385077/1‑52 (MQ=255)
                                            cTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCaac                         >  1:5124203/1‑69 (MQ=255)
                                             tGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCg                        >  1:1763425/1‑71 (MQ=255)
                                             tGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCaa                                        >  1:1343995/1‑55 (MQ=255)
                                             tGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACg                        >  1:302305/1‑71 (MQ=255)
                                               tcTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGGCAAATGCGGCAACTTCGAAAGAGCaac                         <  1:227589/67‑3 (MQ=255)
                                               gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATc                         <  1:2610503/68‑1 (MQ=255)
                                               gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCgg                       <  1:4551957/70‑1 (MQ=255)
                                                cTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAaga                              >  1:4036508/1‑62 (MQ=255)
                                                  gCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAc                   >  1:4940451/1‑71 (MQ=255)
                                                   ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa                                                  <  1:1003907/39‑1 (MQ=255)
                                                    cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTa                    >  1:1963677/1‑68 (MQ=255)
                                                    cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTa                    >  1:751527/1‑68 (MQ=255)
                                                    cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga                 <  1:1587703/71‑1 (MQ=255)
                                                      cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagt               >  1:1326477/1‑71 (MQ=255)
                                                      cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagt               >  1:342421/1‑71 (MQ=255)
                                                         gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc              <  1:685737/69‑1 (MQ=255)
                                                         gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc              <  1:4282139/69‑1 (MQ=255)
                                                          ggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc              <  1:3089739/68‑1 (MQ=255)
                                                           gCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa                                  <  1:1302183/47‑1 (MQ=255)
                                                             cGAGATAAGTAACTCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCt        >  1:1494119/1‑71 (MQ=255)
                                                             cGAGATAAGTAACGCGAGCACCGTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCt        <  1:3426440/71‑1 (MQ=255)
                                                             cGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa                                  <  1:5400427/45‑1 (MQ=255)
                                                               aaaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAc                   <  1:1668361/56‑1 (MQ=255)
                                                               aaaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCttt      >  1:3576672/3‑71 (MQ=255)
                                                                gaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcga            >  1:887751/1‑64 (MQ=255)
                                                                aaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCtttt     >  1:234508/2‑71 (MQ=255)
                                                                aaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCtttt     >  1:2311696/2‑71 (MQ=255)
                                                                aaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCttt      <  1:3762245/69‑1 (MQ=255)
                                                                 aTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCg                                   <  1:676513/40‑1 (MQ=255)
                                                                 aTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCg                                   <  1:329878/40‑1 (MQ=255)
                                                                 aTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCAt                          >  1:854560/1‑49 (MQ=255)
                                                                 aTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttt      <  1:2889611/69‑1 (MQ=255)
                                                                    aGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCg  <  1:1388748/70‑1 (MQ=255)
                                                                    aGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCg  <  1:5149346/70‑1 (MQ=255)
                                                                    aGTAACGCGAGCACCATGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc              <  1:274060/58‑1 (MQ=255)
                                                                    |                                                                     
CCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCG  >  minE/2919403‑2919540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A