Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,919,483 |
A→G |
30.2% |
G71G (GGT→GGC) |
argI ← |
ornithine carbamoyltransferase 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,919,483 | 0 | A | G | 30.2%
| 179.6
/ 103.8
| 139 | G71G (GGT→GGC) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base A (40/57); new base G (32/10); total (72/67) |
| Fisher's exact test for biased strand distribution p-value = 1.87e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01 |
Only 100 of 142 total aligned reads displayed.
GGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCG > minE/2919414‑2919552
|
cggtgtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGccc < 1:1362944/69‑1 (MQ=255)
aGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCt < 1:4949510/71‑1 (MQ=255)
tgtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAcc < 1:5352630/67‑1 (MQ=255)
tccttAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCATAAATAGGTACCGCGCGCGCCCTGGt > 1:5591149/4‑70 (MQ=255)
tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCa < 1:4228580/63‑1 (MQ=255)
cttAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCa > 1:228556/2‑70 (MQ=255)
tAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCaaat > 1:5364924/1‑71 (MQ=255)
tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCatat > 1:4699447/1‑71 (MQ=255)
tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa < 1:4431115/68‑1 (MQ=255)
aTCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGt > 1:5045680/1‑64 (MQ=255)
ttgACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCg > 1:1819107/3‑71 (MQ=255)
tCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa < 1:2831777/65‑1 (MQ=255)
gACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCg > 1:717621/1‑69 (MQ=255)
gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGc < 1:218339/71‑1 (MQ=255)
aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAccc < 1:2929451/56‑1 (MQ=255)
aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAccc < 1:136070/56‑1 (MQ=255)
aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAccc < 1:3902671/56‑1 (MQ=255)
aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCa < 1:3390358/71‑1 (MQ=255)
tctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa < 1:2669303/60‑1 (MQ=255)
ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAcc > 1:3580523/1‑50 (MQ=255)
ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc > 1:2903921/1‑71 (MQ=255)
ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc > 1:153013/1‑71 (MQ=255)
tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCt > 1:145437/1‑51 (MQ=255)
tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCaa > 1:5332485/1‑66 (MQ=255)
tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc > 1:3174424/1‑70 (MQ=255)
tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc < 1:694644/70‑1 (MQ=255)
tgcccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGa < 1:280392/67‑1 (MQ=255)
tgcccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGa < 1:3845007/67‑1 (MQ=255)
tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa < 1:3818374/70‑1 (MQ=255)
tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa < 1:2969478/70‑1 (MQ=255)
gACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa < 1:3811750/69‑1 (MQ=255)
gACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa < 1:207597/69‑1 (MQ=255)
aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc < 1:2384455/66‑1 (MQ=255)
aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc < 1:4730925/66‑1 (MQ=255)
cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaaa > 1:5187240/1‑68 (MQ=255)
cAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCggaa > 1:4806502/1‑57 (MQ=255)
cAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCaa > 1:2786265/1‑60 (MQ=255)
aaTCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAagag > 1:4736921/1‑70 (MQ=255)
aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCa > 1:397055/1‑71 (MQ=255)
aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCa > 1:3479276/1‑71 (MQ=255)
tCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaaa < 1:5384988/65‑1 (MQ=255)
cTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCg > 1:1385077/1‑52 (MQ=255)
cTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCaac > 1:5124203/1‑69 (MQ=255)
tGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCg > 1:1763425/1‑71 (MQ=255)
tGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACg > 1:302305/1‑71 (MQ=255)
tcTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGGCAAATGCGGCAACTTCGAAAGAGCaac < 1:227589/67‑3 (MQ=255)
gCTGCCGCTTGTGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCggg < 1:2123353/71‑1 (MQ=255)
gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATc < 1:2610503/68‑1 (MQ=255)
gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCggg < 1:1695333/71‑1 (MQ=255)
gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCgg < 1:4551957/70‑1 (MQ=255)
gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCgg < 1:3465091/70‑1 (MQ=255)
cTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAaga > 1:4036508/1‑62 (MQ=255)
gCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTa > 1:3230002/1‑70 (MQ=255)
ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa < 1:1307294/39‑1 (MQ=255)
ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa < 1:1003907/39‑1 (MQ=255)
cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTa > 1:751527/1‑68 (MQ=255)
cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga < 1:1587703/71‑1 (MQ=255)
cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagt > 1:1326477/1‑71 (MQ=255)
gggCCGAGTTAAGTAACGCGAGCACCCTGGTCATATGCCGCAACTTCGAAAGAGCATCGGGTAcgagtcg < 1:5222055/70‑1 (MQ=255)
gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:685737/69‑1 (MQ=255)
gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:4282139/69‑1 (MQ=255)
ggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:3089739/68‑1 (MQ=255)
gCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa < 1:1302183/47‑1 (MQ=255)
cGAGATAAGTAACGCGAGCACCGTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCt < 1:3426440/71‑1 (MQ=255)
cGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa < 1:5400427/45‑1 (MQ=255)
aaaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAc < 1:1668361/56‑1 (MQ=255)
aaaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAc < 1:1392408/56‑1 (MQ=255)
aaaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAc < 1:465784/56‑1 (MQ=255)
aaaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCttt > 1:3576672/3‑71 (MQ=255)
gaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcga > 1:887751/1‑64 (MQ=255)
gaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtttt < 1:4420153/71‑1 (MQ=255)
aaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCtttt > 1:3430912/2‑71 (MQ=255)
aaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCtttt > 1:234508/2‑71 (MQ=255)
aaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCttt < 1:3762245/69‑1 (MQ=255)
aTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCg < 1:676513/40‑1 (MQ=255)
aTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCg < 1:329878/40‑1 (MQ=255)
taggTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTc > 1:3678306/4‑71 (MQ=255)
aggTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCtttt > 1:2723965/3‑68 (MQ=255)
aGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCg < 1:5149346/70‑1 (MQ=255)
aGTAACGCGAGCACCATGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:3956849/58‑1 (MQ=255)
aGTAACGCGAGCACCATGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:274060/58‑1 (MQ=255)
gTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtttttag > 1:3370418/1‑67 (MQ=255)
gTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttt < 1:892911/65‑1 (MQ=255)
gTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttt < 1:1094189/65‑1 (MQ=255)
gTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttt < 1:3269978/65‑1 (MQ=255)
tAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTa > 1:4805761/1‑50 (MQ=255)
tAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTa > 1:2063531/1‑50 (MQ=255)
tAACGCGAGCACCGTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTc > 1:3337491/1‑67 (MQ=255)
tAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa < 1:5377826/36‑1 (MQ=255)
tAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa < 1:771263/36‑1 (MQ=255)
aCGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGAt < 1:495332/71‑1 (MQ=255)
aCGCGAGCACCCTGGTCATATGCGTCAACTTCGAAAGAGCATCTGGTAc > 1:2053851/1‑49 (MQ=39)
aCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga > 1:2468374/1‑51 (MQ=255)
aCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGa > 1:3640181/1‑70 (MQ=255)
gAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcg < 1:3494316/51‑1 (MQ=255)
gAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa > 1:4629004/1‑64 (MQ=255)
aGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCt < 1:5239119/55‑1 (MQ=255)
gCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCg > 1:1859077/1‑60 (MQ=255)
gCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGa > 1:4083512/1‑61 (MQ=255)
aCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGcgcg < 1:4041117/70‑1 (MQ=255)
|
GGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCG > minE/2919414‑2919552
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
