Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
364,870 |
A→G |
T220T (ACT→ACC) |
lacZ ← |
beta‑D‑galactosidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 364,870 | 0 | A | G | 94.2%
| 125.9
/ ‑1.9
| 52 | T220T (ACT→ACC) | lacZ | beta‑D‑galactosidase |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base G (0/49); total (3/49) |
| Fisher's exact test for biased strand distribution p-value = 4.52e-05 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01 |
TCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGACGTCACGGAAA > W3110S.gb/364829‑364903
|
tcatTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACga > 1:256238/1‑62 (MQ=255)
tcatTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACga > 1:188175/1‑62 (MQ=255)
tcatTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACga > 1:537473/1‑62 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:491931/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:300833/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:318965/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:324635/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:348754/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:376047/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:39212/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:443576/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:451573/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:473122/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:473611/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:284379/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:516664/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:624932/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:670152/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:679758/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:695825/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:747488/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:747541/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:76553/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:775059/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:978902/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:999434/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1230391/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1019069/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1021230/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1041387/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1104466/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1105224/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:11119/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1177459/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:122481/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1229148/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1230000/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1012529/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1246602/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1248126/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1271942/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1414937/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:1442171/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:148790/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:160064/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:189323/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:206204/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:230204/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:247934/51‑1 (MQ=255)
aaaTCGCTGATTTGCGTGGTCGGGTTATGCAGCAACGAGACGTCACGGaaa < 1:593846/51‑1 (MQ=255)
aaTCGCTGATTTGCGTGGTCGGTTTATGCAGCAACGAGACGTCACGGaaa < 1:74772/50‑1 (MQ=255)
tttGCGTGGTCGGTTTATGCAGCAACGAGACGTCACTGaaa < 1:1272766/41‑1 (MQ=37)
|
TCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGACGTCACGGAAA > W3110S.gb/364829‑364903
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
