Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 439,984 G→A G114G (GGC→GGT)  ispA ← geranyltranstransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb439,9840GA100.0% 341.2 / NA 121G114G (GGC→GGT) ispAgeranyltranstransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (121/0);  total (121/0)

Only 100 of 121 total aligned reads displayed.

GCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATC  >  W3110S.gb/439935‑439996
                                                 |            
gCATATCGGCATCGCTTAGAATCGTGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:687566/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGATAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1241040/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAAt   >  1:385302/1‑61 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAAt   >  1:523052/1‑61 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAAt   >  1:1451008/1‑61 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:613318/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:620000/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:602673/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:588272/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:585221/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:575072/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:574982/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:57197/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:546294/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:545550/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:510576/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:510441/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:486652/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:480037/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:422798/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:421896/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:384636/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:375848/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:351915/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:324901/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:320880/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:810940/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:985830/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:980218/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:950313/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:906368/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:857992/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:856469/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:854338/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:850930/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:833199/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:824591/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:823802/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:615701/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:791719/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:772257/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:771201/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:756611/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:74418/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:735632/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:696806/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:67531/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:647858/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:631882/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:312124/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1162846/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1309981/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1001785/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1303263/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1300821/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:127520/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1262079/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1252838/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1241583/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1232466/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1224909/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1164874/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1308130/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1160653/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1156382/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1148518/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1129130/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:112052/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1113404/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1095689/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1066682/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1065030/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1044752/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1021142/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:174399/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:307944/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:304722/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:272129/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:264702/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:262605/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:249005/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:213052/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:200791/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:189135/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1328451/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:150180/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1470141/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:146466/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1448366/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1448354/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1429048/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1411654/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1398747/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1373263/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1364235/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1344071/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACAAGCGAGAATc  >  1:1237852/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGAAAAGCGTCACCAGCGAGAATc  >  1:343965/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGACAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:919746/1‑62 (MQ=255)
gCATATCGGCACCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1102123/1‑62 (MQ=255)
                                                 |            
GCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATC  >  W3110S.gb/439935‑439996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A