Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 478,855 G→A I96I (ATC→ATT)  maa ← maltose O‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb478,8550GA100.0% 283.8 / NA 102I96I (ATC→ATT) maamaltose O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/102);  total (0/102)

Only 100 of 102 total aligned reads displayed.

ATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTAT  >  W3110S.gb/478849‑478910
      |                                                       
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:511053/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:622485/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:615978/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:614516/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:576487/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:575841/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:570256/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:558519/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:528502/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:528368/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:521021/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:517225/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:639137/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:449025/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:440370/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:426881/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:419790/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:41728/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:40527/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:384517/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:379060/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1011043/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:312394/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:307212/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:798203/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:97828/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:938753/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:93014/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:922206/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:918665/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:886851/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:872015/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:849272/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:837685/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:825882/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:80794/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:37566/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:77114/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:728618/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:725947/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:70431/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:693306/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:692622/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:689110/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:67827/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:665156/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:656558/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:640985/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1218174/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1324071/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1297491/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1274070/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1271753/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1256364/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1247904/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1243988/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1232908/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1224937/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1222741/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1222466/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:296078/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1201866/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1194450/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1184758/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1170680/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1133621/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1125286/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1109935/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1076858/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1057553/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1032412/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1025449/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1426450/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:198864/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:168113/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:154778/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:202254/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:14720/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:203758/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:205487/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1438305/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1431794/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1412567/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:2418/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1409422/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:258938/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1386378/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1368214/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1355460/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:305870/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1334347/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCCAAAAAattat  <  1:1440577/62‑1 (MQ=255)
aTCACCAATGCGAATAGGGCAGACATCAAGCAACACGCAATCGAAGTTGGCGAAAAAattat  <  1:1308122/62‑1 (MQ=255)
 tCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:445605/61‑1 (MQ=255)
 tCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:469811/61‑1 (MQ=255)
 tCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:766641/61‑1 (MQ=255)
 tCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:542565/61‑1 (MQ=255)
 tCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:670592/61‑1 (MQ=255)
 tCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAattat  <  1:1303847/61‑1 (MQ=255)
 tCACCAATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGGTGGCGAAAAAattat  <  1:110975/61‑1 (MQ=255)
      |                                                       
ATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTAT  >  W3110S.gb/478849‑478910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A