Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 534,504 G→A E455E (GAG→GAA)  gcl → glyoxylate carboligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb534,5040GA100.0% 301.5 / NA 107E455E (GAG→GAA) gclglyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (107/0);  total (107/0)

Only 100 of 107 total aligned reads displayed.

GCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGT  >  W3110S.gb/534463‑534523
                                         |                   
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGcc               >  1:788481/1‑47 (MQ=37)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGTCGCACAGt  >  1:328646/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGTGCACAGt  >  1:794114/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAg   >  1:485344/1‑60 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:517221/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:745886/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:725563/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:698588/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:638369/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:617648/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:603174/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:602343/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:569972/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:540457/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:517801/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:769437/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:493945/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:478062/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:472685/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:46101/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:458977/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:440343/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:424236/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:407783/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:354841/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:346869/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:846549/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:98353/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:974073/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:960839/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:938343/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:93617/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:926739/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:925316/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:920044/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:914866/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:862656/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:848082/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:752605/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:842/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:838936/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:824459/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:815458/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:810134/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:796045/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:792670/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:790091/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:789783/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:774390/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:339410/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:121467/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1344772/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1330072/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1329707/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:131139/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1287065/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1278281/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:126999/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1266627/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1243288/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:123945/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1224183/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1219030/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:336411/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1191366/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1167626/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1144687/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1122638/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1087648/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1080670/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1079617/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1071768/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1069370/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1049816/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1035753/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:160901/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1013709/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:327799/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:277234/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:244501/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:237037/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:231239/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:2269/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:201791/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:190077/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:179046/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:177153/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:17110/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:153632/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1460009/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1456871/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1451664/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1420964/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:141953/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1409366/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1402156/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1353579/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1347722/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACTTACAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1346982/1‑61 (MQ=255)
gCGATTTCCGGTGACTTCGACATCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:583781/1‑61 (MQ=255)
                                         |                   
GCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGT  >  W3110S.gb/534463‑534523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A