Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
891,095 |
T→C |
E27E (GAA→GAG) |
grxA ← |
glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 891,095 | 0 | T | C | 77.8%
| 241.4
/ 56.8
| 153 | E27E (GAA→GAG) | grxA | glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) |
| Reads supporting (aligned to +/- strand): ref base T (0/34); new base C (119/0); total (119/34) |
| Fisher's exact test for biased strand distribution p-value = 8.20e-35 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 153 total aligned reads displayed.
TATCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTT > W3110S.gb/891064‑891125
|
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:1141858/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:601345/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:1320879/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:641480/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:705607/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:764898/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:833803/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:85286/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:1163819/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:1163598/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:112948/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:311884/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:690502/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:1092620/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:1072691/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:669363/62‑1 (MQ=255)
tatCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:971016/62‑1 (MQ=255)
atCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:174722/61‑1 (MQ=255)
atCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:492800/61‑1 (MQ=255)
atCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:748163/61‑1 (MQ=255)
atCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCtttt < 1:1471658/61‑1 (MQ=255)
tgatactggAAATCATCGCGCTCATTGCTCAAttt > 1:970854/1‑35 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:555121/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:662353/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:657833/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:650898/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:599092/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:586341/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:573405/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:571268/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:708151/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:554015/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:551258/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:526490/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:507313/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:490536/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:762941/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:985202/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:9557/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:945792/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:915402/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:911607/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:872798/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:871131/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:774813/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:670376/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:74913/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:73592/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:724020/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:71993/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:403640/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:701659/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:700834/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:68269/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1192637/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:485064/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1350862/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1349528/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1333549/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1332991/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1320432/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1312342/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1295844/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1276999/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1259872/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1203964/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1356677/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1174018/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1123873/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1120354/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1087862/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1053908/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1045438/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1037498/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1026194/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1022863/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1019937/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:255964/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:458263/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:457520/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1001528/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:35895/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:339224/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:304374/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:277955/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:274602/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1358187/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:238592/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:232669/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:204305/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:187356/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:162148/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1464713/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1425951/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:1405191/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtct > 1:139818/1‑37 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtc > 1:649889/1‑36 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtc > 1:58441/1‑36 (MQ=37)
tgatactggAAATCATCGCGCTCATTGCTCAATTtc > 1:297262/1‑36 (MQ=37)
tgatactggAAATCATCGCGCTCATAGCTCAATTtct > 1:864053/1‑37 (MQ=25)
|
TATCTACATACTGATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTT > W3110S.gb/891064‑891125
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
