Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,162,391 C→G S246S (TCG→TCC)  fhuE ← ferric‑rhodotorulic acid outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,162,3910CG100.0% 310.4 / NA 110S246S (TCG→TCC) fhuEferric‑rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (0/110);  total (0/110)

Only 100 of 110 total aligned reads displayed.

CTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAT  >  W3110S.gb/1162369‑1162430
                      |                                       
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:538398/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:663036/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:644957/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:631003/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:627417/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:596823/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:595744/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:584730/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:568977/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:55412/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:547833/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:541445/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:663601/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:537563/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:521538/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:503405/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:497547/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:493279/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:491908/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:470612/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:457699/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:397592/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:397005/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:349053/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:838834/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:966521/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:957878/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:954239/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:946727/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:938159/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:921389/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:91297/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:911996/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:90864/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:857887/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:853826/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:437487/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:807005/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:770512/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:751251/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:750318/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:739587/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:729162/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:722227/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:720531/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:69085/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:675027/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1180414/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:129937/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1257229/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:317997/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:124464/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1238043/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1199822/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1198553/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1184012/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1182833/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1181699/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1181533/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1250543/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1173380/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1161025/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1139674/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1121040/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1086795/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1082477/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1082237/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1069259/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1038553/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1001346/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:221771/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:282800/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:25462/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:253365/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:247245/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:234128/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:23130/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:18327/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:163665/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:162106/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1460550/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1466942/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:144924/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1425299/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1431341/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGGCCAGCCAt  <  1:1037914/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGGCCAGCCAt  <  1:465793/62‑1 (MQ=255)
cTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGGCCAGCCAt  <  1:557763/62‑1 (MQ=255)
cTAAATCAGCACCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1274670/62‑1 (MQ=255)
 tAAATCAGCATCGACTATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1379776/61‑1 (MQ=255)
 tAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:835926/61‑1 (MQ=255)
 tAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1337519/61‑1 (MQ=255)
 tAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1000347/61‑1 (MQ=255)
 tAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:941411/61‑1 (MQ=255)
 tAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1345313/61‑1 (MQ=255)
 tAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:102356/61‑1 (MQ=255)
 tAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGGCCAGCCAt  <  1:517223/61‑1 (MQ=255)
     tCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1135940/57‑1 (MQ=255)
       aGCATAGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:753391/55‑1 (MQ=255)
             gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt  <  1:1101764/49‑1 (MQ=255)
                      |                                       
CTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAT  >  W3110S.gb/1162369‑1162430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A