Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,248,524 |
2 bp→TT |
coding (429‑430/1698 nt) |
treA ← |
periplasmic trehalase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,248,524 | 0 | C | T | 100.0%
| 261.8
/ NA
| 95 | E144K (GAA→AAA) | treA | periplasmic trehalase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (95/0); total (95/0) |
| * | W3110S.gb | 1,248,525 | 0 | C | T | 100.0%
| 261.9
/ NA
| 95 | P143P (CCG→CCA) | treA | periplasmic trehalase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (95/0); total (95/0) |
TACCTCGCGAAAGCGTCCGCCCGGCACGACATAAGGTTCCGGCAGCGGTAACAGAGAATCCC > W3110S.gb/1248486‑1248547
||
taCCTCTCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1234696/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATagag > 1:990175/1‑56 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATag > 1:1217029/1‑54 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATTGAGAATccc > 1:259568/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGTGAATccc > 1:1289247/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:391877/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:545538/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:538836/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:475985/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:474502/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:452077/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:443481/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:440133/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:43871/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:409814/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:396086/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:577554/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:385002/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:378632/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:378064/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:375883/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:374401/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:338604/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:337396/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:330409/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:322713/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:305711/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:736190/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:991782/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:982275/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:977969/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:971375/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:839822/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:833609/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:814276/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:782600/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:778211/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:776453/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:740278/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:548670/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:704490/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:689046/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:65188/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:649844/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:646567/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:638029/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:621154/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:582055/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:275800/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:575279/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:111030/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:129571/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1272119/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1235873/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1230691/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1227741/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:121575/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:120423/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1190487/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1182997/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1151874/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:11253/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:13134/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1107853/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1093849/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1090710/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1087142/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1085026/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1059947/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1054577/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1043681/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1029284/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1027309/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:151349/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:292169/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1000665/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:275413/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:26276/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:249321/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:243423/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:223945/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:22223/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:207723/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:186656/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:302567/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1467476/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1449345/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1446625/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1442315/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1410625/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1408525/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1380970/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1380230/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:1365526/1‑62 (MQ=255)
taCCTCGCGAAAACGTCCGCCGGGCACGACATAAGGTTTTGGCAGCGGTAATAGAGAATccc > 1:135272/1‑62 (MQ=255)
||
TACCTCGCGAAAGCGTCCGCCCGGCACGACATAAGGTTCCGGCAGCGGTAACAGAGAATCCC > W3110S.gb/1248486‑1248547
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
