Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,390,948 G→A R102K (AGA→AAA)  ycjG → L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,390,9480GA100.0% 350.0 / NA 126R102K (AGA→AAA) ycjGL‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/126);  total (0/126)

Only 100 of 126 total aligned reads displayed.

GCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAC  >  W3110S.gb/1390923‑1390984
                         |                                    
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGTCATAAc  <  1:495775/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATaag  <  1:1051876/61‑2 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:516150/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:699159/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:68506/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:677634/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:6738/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:633954/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:632444/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:617029/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:608447/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:568403/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:56569/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:54812/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:547804/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:71086/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:514915/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:510595/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:487754/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:468745/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:458046/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:456071/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:454535/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:436651/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:426878/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:391062/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:813501/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:939098/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:921318/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:909667/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:903614/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:897672/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:888720/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:8858/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:885252/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:875328/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:843754/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:832784/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:36823/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:813023/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:802515/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:795376/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:782026/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:779475/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:777737/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:771551/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:768505/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:75877/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:757144/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:748608/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1233375/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1015918/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1333074/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1331213/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1322729/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1320044/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1319749/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1309078/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1273968/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1254714/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1250326/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1239440/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1235109/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1356270/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1232054/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1219726/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1183770/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1176527/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1115486/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1113209/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1094046/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1085049/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1076825/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1053559/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1047028/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:241931/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:333203/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:32297/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:320212/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:312462/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:303074/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:301976/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:280662/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:256246/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:255519/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:24874/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1396831/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:209657/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:207863/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:202640/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:18403/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1467614/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1452252/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1443801/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1437449/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1420457/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1419566/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1397556/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGCCATAAc  <  1:497466/61‑1 (MQ=38)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGCCTGATTTGATCGGCATAAc  <  1:1100636/61‑1 (MQ=38)
                         |                                    
GCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAC  >  W3110S.gb/1390923‑1390984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A