Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,500,574 T→C L55L (CTA→CTG)  yfdY ← predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,500,5740TC100.0% 295.7 / NA 105L55L (CTA→CTG) yfdYpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (105/0);  total (105/0)

Only 100 of 105 total aligned reads displayed.

TCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTT  >  W3110S.gb/2500531‑2500592
                                           |                  
tcaAGCCACAAGCCGGGGTAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:888071/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCATGAAACATAAATATCCCCGTCACCAGTTCAt             >  1:153941/1‑51 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTc               >  1:843620/1‑49 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCAtt            >  1:1422411/1‑52 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTGCCGCCAGtt  >  1:1131819/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:443977/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:594472/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:589727/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:572240/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:554464/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:545394/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:510314/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:506422/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:506342/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:489711/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:486045/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:468242/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:632723/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:429421/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:399074/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:386911/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:381994/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:345321/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:302284/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:292007/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:280424/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:279466/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:81120/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:978263/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:977251/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:973389/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:957681/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:943977/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:941352/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:91455/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:890484/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:832022/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:830589/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:82897/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:597674/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:809272/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:808608/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:80025/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:75143/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:709564/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:664217/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:649645/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:646806/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:644318/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:26343/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:624367/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1158014/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1315829/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1293543/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1248876/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1240175/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1216716/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1215058/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1214016/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1197145/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1195425/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1194092/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1300965/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1149156/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1142100/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1133101/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1122976/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1121544/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1105862/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1074028/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1052912/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1014094/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1006597/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1411818/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:260897/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:238507/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:236458/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:233857/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:229829/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:220335/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:191059/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:17779/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:173086/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1444587/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1430507/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1319803/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1409723/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1402237/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1401471/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1391178/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1389545/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1368852/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGtt  >  1:1323055/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGt   >  1:173690/1‑61 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGt   >  1:1114091/1‑61 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAGt   >  1:1277768/1‑61 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTAACGCCAGtt  >  1:1402045/1‑62 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATACCCGTCACCAGTTCa              >  1:765612/1‑50 (MQ=255)
tcaAGCCACAAGCCGGGGCAAGAAACATAAATATACCCGTCACCAGTTCATTACCGCCAGtt  >  1:1004283/1‑62 (MQ=255)
tcaAGCCACAAGCAGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCGCCAg    >  1:752468/1‑60 (MQ=255)
                                           |                  
TCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTT  >  W3110S.gb/2500531‑2500592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A