Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,583,867 G→A V105V (GTC→GTT)  aegA ← fused predicted FeS binding subunit and predicted NAD/FAD‑binding subunit of oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,583,8670GA92.7% 258.5 / 5.5 109V105V (GTC→GTT) aegAfused predicted FeS binding subunit and predicted NAD/FAD‑binding subunit of oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (8/0);  new base A (101/0);  total (109/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 109 total aligned reads displayed.

CGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTACCAAAAGGACAGGCC  >  W3110S.gb/2583831‑2583897
                                    |                              
cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGt                  >  1:732203/1‑51 (MQ=255)
cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGt                  >  1:1178031/1‑51 (MQ=255)
cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTa                 >  1:516263/1‑52 (MQ=255)
cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTa                 >  1:239119/1‑52 (MQ=255)
cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTa                 >  1:571551/1‑52 (MQ=255)
cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTa                 >  1:73264/1‑52 (MQ=255)
cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTa                 >  1:848294/1‑52 (MQ=255)
cGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTa                 >  1:863613/1‑52 (MQ=255)
     cttttacttttaCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:43414/1‑62 (MQ=255)
     cttttacttttaCTACCGCAACCGGTGTAAGAACGCTTTGCATCGTACCAAATGGACAGGcc  >  1:1032604/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTAcc               >  1:1012801/1‑49 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATgg         >  1:420429/1‑55 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:957974/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:586439/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:573135/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:916027/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:537298/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:94106/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:532429/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:531399/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:526045/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:522429/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:660660/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:508243/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:508124/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:505755/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:496058/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:464182/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:442465/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:969058/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:400862/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:805548/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:854405/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:875387/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:876024/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:838193/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:836065/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:828691/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:821674/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:809929/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:808538/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:867455/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:800517/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:797036/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:78501/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:883905/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:894208/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:715845/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:677070/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:674290/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:666431/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1212296/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:400329/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1401878/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1396185/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1388554/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1309636/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1309144/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1300109/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1296792/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1284921/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1255767/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1254798/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1414623/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1199500/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1166420/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1147839/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1144065/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1130679/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1092165/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1081695/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1053813/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1038361/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1024287/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:268697/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:362296/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:360803/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:360568/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:355322/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:314296/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:312402/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:284719/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:274880/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:270475/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1444305/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:255499/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:241262/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:238572/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:230546/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:191246/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:182065/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:16451/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:147945/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1458094/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:1445963/1‑62 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGc   >  1:1080037/1‑61 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGc   >  1:693699/1‑61 (MQ=255)
     cTTTTACTTTTCCTACCGCAACGGGTGTCAGAAAGATTTGCATCGTACCAAATGGACAGGcc  >  1:919496/1‑62 (MQ=255)
     cTTTTACATTTCCTACCGCAACGGGTGTCAGAACg                             >  1:1152634/1‑35 (MQ=19)
     cTTTCACTTTTCCTACCGCAACGGGTGTCAGAACGATTTGCATCGTACCAAATGGACAGGcc  >  1:605768/1‑62 (MQ=255)
                                    |                              
CGTGGCTTTTACTTTTCCTGCCGCGACGGGTGTCAGGACGATTTGCATCGTACCAAAAGGACAGGCC  >  W3110S.gb/2583831‑2583897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A