Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,911,612 |
G→A |
F232F (TTC→TTT) |
relA ← |
(p)ppGpp synthetase I/GTP pyrophosphokinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,911,612 | 0 | G | A | 99.3%
| 390.0
/ ‑4.8
| 141 | F232F (TTC→TTT) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (0/140); minor base T (0/1); total (0/141) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 |
Only 100 of 141 total aligned reads displayed.
CAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTT > W3110S.gb/2911600‑2911661
|
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCTGTCGAGACGGCGTTCATGCAGCAGtt < 1:455808/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGcagcagat < 1:84571/62‑3 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:454112/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:587566/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:575081/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:563026/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:544650/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:537743/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:52757/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:505047/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:482066/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:469495/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:603993/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:425062/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:383503/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:380372/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:364768/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:339499/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:31598/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:303317/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:297452/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:814493/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:958564/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:940760/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:93814/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:932362/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:916376/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:914889/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:899151/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:89904/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:826712/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1022570/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:81192/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:810078/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:791988/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:705804/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:703583/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:666651/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:623707/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:604987/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1420334/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1243791/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1267261/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1281950/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1226873/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1282099/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1306657/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1226453/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:121870/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1192999/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1353580/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1188513/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1392803/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1402934/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1404477/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1185034/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1434154/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1159025/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1448012/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1148101/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:146663/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:168888/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:200352/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:214195/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:230518/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1112230/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1102094/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:246680/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1093702/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1033825/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:262104/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCCAGACGGCGTTCATGCAGCAGtt < 1:965107/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGGCGAGACGGCGTTCATGCAGCAGtt < 1:736936/62‑1 (MQ=255)
cAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGGCGAGACGGCGTTCATGCAGCAGtt < 1:462655/62‑1 (MQ=255)
cAGATGGCCGACAAAATCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:731454/62‑1 (MQ=255)
aGATGGCCGACTAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:449179/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGTGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1213405/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCTGTCGAGACGGCGTTCATGCAGCAGtt < 1:668800/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1202555/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:275453/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1015051/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:286317/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:296010/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:238690/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:304482/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:417211/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:857220/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:138010/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:842898/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1343917/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:627548/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1210731/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:809033/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1333432/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:763535/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:707684/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:1221856/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:596276/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGtt < 1:627578/61‑1 (MQ=255)
aGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGGTCATGCAGCAGtt < 1:564010/61‑1 (MQ=255)
|
CAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTT > W3110S.gb/2911600‑2911661
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
