Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,032,319 T→C V504V (GTA→GTG)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,032,3190TC100.0% 341.9 / NA 120V504V (GTA→GTG) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (120/0);  total (120/0)

Only 100 of 120 total aligned reads displayed.

ATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGG  >  W3110S.gb/3032309‑3032359
          |                                        
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGt           >  1:151836/1‑42 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgt  >  1:712860/1‑50 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:622809/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:735409/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:733439/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:715302/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:703020/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:672226/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:662020/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:661678/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:653202/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:636932/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:629731/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:74447/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:605266/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:574386/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:538450/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:52538/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:519523/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:51420/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:502394/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:502291/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:50066/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:496546/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:475760/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:472187/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:860223/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:972467/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:968216/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:959045/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:923856/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:917686/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:912076/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:907346/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:906175/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:904967/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:904417/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:888919/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:8805/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:449283/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:85534/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:847064/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:840435/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:831852/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:806739/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:805965/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:775539/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:760041/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:75651/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:752295/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:75084/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1198346/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1026784/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1362232/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1354074/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1347605/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:132884/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1285691/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1280919/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1252799/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1239001/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1206965/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1201889/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1366892/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1192130/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1186411/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1142155/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1127639/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1126388/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1095257/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1090299/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1080363/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1065876/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1039443/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1029843/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:28103/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:442522/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:440724/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:433069/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:432629/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:427644/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:407561/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:407323/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:393298/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:385050/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:375202/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:337029/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:289202/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1388392/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:258840/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:229000/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:169738/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:163885/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:159677/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1467803/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1436590/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1424811/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg  >  1:1424562/1‑51 (MQ=255)
aTGCTTATTTCACCGGACGCATCGCCGGGAACAGAATAACGGCGCGGATgg  >  1:1305080/1‑51 (MQ=25)
aTGCTTATTTCACCGGACCCATCGCCGGGAACAGAATAACGTCGCGGATg   >  1:1280944/1‑50 (MQ=25)
          |                                        
ATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGG  >  W3110S.gb/3032309‑3032359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A