Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,355,773 T→G V429V (GTT→GTG)  gltB → glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,355,7730TG100.0% 375.1 / NA 131V429V (GTT→GTG) gltBglutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (0/131);  total (0/131)

Only 100 of 131 total aligned reads displayed.

GCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACC  >  W3110S.gb/3355755‑3355815
                  |                                          
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:453594/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:546990/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:539948/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:528153/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:514269/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:498370/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:495806/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:494253/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:474950/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:465617/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:465084/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:460290/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:55040/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:445254/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:399450/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:395049/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:383866/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:381841/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:33002/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:30914/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1011408/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:27591/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:263836/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:262891/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:757029/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:991222/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:988187/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:983502/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:965916/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:964171/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:960090/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:896600/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:869129/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:863124/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:820730/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:800483/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:29733/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:747251/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:745160/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:700990/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:678188/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:654418/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:636501/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:634371/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:622793/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:606421/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:565682/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:117876/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1256692/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1255026/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:262234/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1242443/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1240715/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1229125/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1223769/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1220842/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1211157/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1194024/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:118184/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1245692/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1172821/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1167990/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:116518/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1155492/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1133776/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1128303/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1101908/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:107666/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:107060/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1063743/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1303878/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:242730/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:238817/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:220237/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:215117/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:175699/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1459998/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1455878/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1447911/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1444408/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1418007/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1404176/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:14001/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1289459/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1317863/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1349159/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1377596/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1369474/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1349793/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCCCATTCTACACTCGGCAGAAAcc  <  1:364905/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGAGCATTCTACACTCGGCAGAAAcc  <  1:613372/61‑1 (MQ=255)
 ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1196712/60‑1 (MQ=255)
 ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:56641/60‑1 (MQ=255)
 ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:338231/60‑1 (MQ=255)
 ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1382870/60‑1 (MQ=255)
  cAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:892405/59‑1 (MQ=255)
        aaCTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:1451809/53‑1 (MQ=255)
          cTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:63718/51‑1 (MQ=255)
              tGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:924871/47‑1 (MQ=255)
              tGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc  <  1:119350/47‑1 (MQ=255)
                  |                                          
GCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACC  >  W3110S.gb/3355755‑3355815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A