Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,543,189 A→C K261Q (AAG→CAG)  rhaD → rhamnulose‑1‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,543,1890AC100.0% 196.0 / NA 69K261Q (AAG→CAG) rhaDrhamnulose‑1‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (0/69);  total (0/69)

TGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCAAGCGTTTCGGC  >  W3110S.gb/3543139‑3543200
                                                  |           
tGGGCGGCTTGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:331990/62‑1 (MQ=255)
tGGGCGGCATGAAACAGATCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1023461/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:585425/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:220453/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:550131/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:482637/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:465002/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:459986/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:431918/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:390585/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:349118/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:335998/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:323223/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:304196/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:28032/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:232547/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:993294/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:604449/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:648396/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:654386/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:682766/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:690401/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:72208/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:735653/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:784770/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:793247/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:79931/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:800193/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:824463/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:835373/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:889684/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:932131/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1006819/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1314640/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:104685/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1077168/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1097723/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1135589/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1164322/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1204091/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1207518/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1222585/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1232965/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1234190/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1240977/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1299180/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:200394/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1326188/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1446163/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:192713/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:183335/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:176216/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:16670/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:155210/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1453662/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1333292/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1445149/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1426316/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1365120/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1361438/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1354371/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1347825/62‑1 (MQ=255)
tGGGCGGCATGAAACAGACCATAAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:638936/62‑1 (MQ=255)
tGGGCGGCAAGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:676900/62‑1 (MQ=255)
 gggCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:789290/61‑1 (MQ=255)
 gggCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1307301/61‑1 (MQ=255)
 gggCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:182723/61‑1 (MQ=255)
 gggCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCCAGCGTTTCGGc  <  1:905881/61‑1 (MQ=255)
     ggcATGAAACAGACCATCAGCCGTGAAGAGTAGATAGCGCTCGGCCAGCGTTTCGGc  <  1:1194023/57‑1 (MQ=255)
                                                  |           
TGGGCGGCATGAAACAGACCATCAGCCGTGAAGAGTTGATAGCGCTCGGCAAGCGTTTCGGC  >  W3110S.gb/3543139‑3543200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A