Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,649,938 T→C A380A (GCT→GCC)  hemY → predicted protoheme IX synthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,649,9380TC100.0% 226.2 / NA 80A380A (GCT→GCC) hemYpredicted protoheme IX synthesis protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (80/0);  total (80/0)

CCGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCTGCAGCTATGCGTCGCGACGGTTTG  >  W3110S.gb/3649901‑3649962
                                     |                        
ccGACGCGCTGGACAGATTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:708487/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGc                        >  1:1136733/1‑40 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTc             >  1:581959/1‑51 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGttt   >  1:145957/1‑61 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGttt   >  1:316320/1‑61 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGttt   >  1:798243/1‑61 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGttt   >  1:571285/1‑61 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGttt   >  1:827363/1‑61 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGttga  >  1:1185381/1‑60 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:569943/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:698958/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:642520/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:641084/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:580707/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:578300/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:729002/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:549592/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:49529/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:492792/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:492771/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:491509/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:488251/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:47700/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:85414/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:92304/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:921127/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:909466/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:906503/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:900489/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:885405/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:883959/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:880788/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:856112/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:709493/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:830185/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:795245/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:788057/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:781671/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:757807/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:750327/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:440205/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:711279/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1230063/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1393944/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1352659/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1332706/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1292284/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1288570/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:128414/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1277059/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1275975/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:127232/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:140609/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1223727/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1208287/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1188434/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1164045/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1138045/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1134381/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1133861/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1028986/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:169144/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:428576/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:405982/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:376722/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:345124/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:312633/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:284028/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:216763/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:212799/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:161204/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:150963/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1446228/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1427632/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1427041/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1415204/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:1408774/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGCGATGCGTCGCGACGGTTTg  >  1:455029/1‑62 (MQ=255)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGCAGAGATGCGTCGCGACGGTTTg  >  1:728473/1‑62 (MQ=38)
ccGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCCGAAGCGATGCGTCGCGACGGATTg  >  1:1008951/1‑62 (MQ=255)
                                     |                        
CCGACGCGCTGGACAGACTGCACAAGCCGGAAGAAGCTGCAGCTATGCGTCGCGACGGTTTG  >  W3110S.gb/3649901‑3649962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A