Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,765,352 G→A A32A (GCG→GCA)  dgoR → predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,765,3520GA100.0% 269.9 / NA 97A32A (GCG→GCA) dgoRpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/97);  total (0/97)

AATACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCGGAACTCTGTGAGGAGTTTGCAACCTCG  >  W3110S.gb/3765318‑3765379
                                  |                           
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGTGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1292350/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTTAGGAGTTTGCAACCTcg  <  1:1229739/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:55764/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:692225/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:68537/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:680372/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:672677/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:645841/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:633437/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:619033/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:584433/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:572870/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:55894/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:76435/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:550235/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:547863/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:53462/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:53232/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:531542/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:529830/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:519059/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:484231/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:476530/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:459713/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:395243/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:868367/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:999040/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:981733/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:97216/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:970830/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:956398/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:922868/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:9179/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:911344/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:895686/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:875884/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:868534/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:75757/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:854170/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:841278/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:836089/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:829564/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:823037/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:814418/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:793073/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:786110/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:776175/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:389379/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:116378/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1332331/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1002510/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1329566/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1329290/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1323492/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1254434/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1247728/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1240198/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1201033/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1199905/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1332170/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1154882/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1153656/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1123568/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1101573/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1082498/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1056343/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:105246/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1052332/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1041352/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1007590/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:219586/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:382377/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:381322/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:379588/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:376094/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:346788/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:326784/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:3210/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:291574/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:225645/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:175766/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:168711/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:168614/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1437526/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1429359/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1428518/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1412854/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1392163/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1391654/62‑1 (MQ=255)
aaTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1342921/62‑1 (MQ=255)
aaTACGTGCCAGCCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1336639/62‑1 (MQ=255)
aaTACGTCCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1060899/62‑1 (MQ=255)
 aTACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:1245587/61‑1 (MQ=255)
  tACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:904621/60‑1 (MQ=255)
   aCGTGCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:884340/59‑1 (MQ=255)
       gCCAGGCTCGCCGCTTCCGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:766052/55‑1 (MQ=255)
                        cGGCTGAGGCAGAACTCTGTGAGGAGTTTGCAACCTcg  <  1:260518/38‑1 (MQ=255)
                                  |                           
AATACGTGCCAGGCTCGCCGCTTCCGGCTGAGGCGGAACTCTGTGAGGAGTTTGCAACCTCG  >  W3110S.gb/3765318‑3765379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A