Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,032,323 G→A N548N (AAC→AAT)  zntA ← zinc, cobalt and lead efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,032,3230GA100.0% 313.3 / NA 111N548N (AAC→AAT) zntAzinc, cobalt and lead efflux system
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (111/0);  total (111/0)

Only 100 of 111 total aligned reads displayed.

CGCGCAGGGTATCCTGTAACGCAATGACACCAAGCACGTCATCGTTACGTACTACCAGCACT  >  W3110S.gb/4032280‑4032341
                                           |                  
cgcgcAGGGTATCCTGCAACGCAATGCCACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:474372/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACg               >  1:606283/1‑49 (MQ=38)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTAcc        >  1:401954/1‑56 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCAc   >  1:661246/1‑61 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:414677/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:541589/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:497697/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:485265/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:469424/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:464217/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:456821/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:453449/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:44484/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:431991/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:556159/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:410833/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:402295/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:390098/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:38195/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:374761/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:372167/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:283246/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:26318/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:248943/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:231766/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1003344/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:83564/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:985306/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:984001/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:957674/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:933659/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:933221/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:914393/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:872339/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:867838/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:858369/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:85219/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:850229/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:547915/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:82165/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:783842/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:740875/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:73405/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:700695/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:671034/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:64886/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:610414/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:586475/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:209846/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1178444/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:20546/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:128542/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1277117/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1264862/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1252424/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1242422/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1239575/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1207363/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1200916/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1197146/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1182552/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1182135/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1290629/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:117374/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1160176/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1149424/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1119335/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1103167/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1095014/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:108001/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1064498/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1043517/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1030342/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1007411/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1440862/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:203466/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:198937/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:195707/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:178168/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:176508/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:172033/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:166299/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:15528/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1461915/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:131058/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1427161/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:141429/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1402956/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1399176/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:139123/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1388092/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1387929/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1361714/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1358229/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1353048/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTAACAGCACt  >  1:187682/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAAAGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:359213/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACGCAAAGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1311146/1‑62 (MQ=255)
cgcgcAGGGTATCCTGCAACCCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt  >  1:1171635/1‑62 (MQ=255)
cgcgcAGGGGATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGaactacaa       >  1:730447/1‑55 (MQ=255)
                                           |                  
CGCGCAGGGTATCCTGTAACGCAATGACACCAAGCACGTCATCGTTACGTACTACCAGCACT  >  W3110S.gb/4032280‑4032341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A