Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 826 0.0 88706 74.7% 66263 57.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 546,860 G→T T319T (ACG→ACT)  fdrA → predicted acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb546,8600GT100.0% 214.0 / NA 112T319T (ACG→ACT) fdrApredicted acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (112/0);  total (112/0)

Only 100 of 112 total aligned reads displayed.

GGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAG  >  W3110S.gb/546818‑546879
                                          |                   
ggATGATGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:79640/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGTCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:48539/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGAt    >  1:67431/1‑60 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATa   >  1:46591/1‑61 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATa   >  1:65171/1‑61 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATa   >  1:49992/1‑61 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:61675/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:68409/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:68407/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:68469/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:67257/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:67248/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:49728/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:64463/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:63293/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:62165/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:51845/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:61474/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:59761/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:58304/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:58070/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:55238/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:54485/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:53592/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:5294/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:79238/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:86426/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:85610/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:85318/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:83620/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:83487/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:81680/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:81360/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:81206/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:80168/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:80114/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:79838/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:68465/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:76777/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:7390/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:73595/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:73357/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:73173/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:73097/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:72231/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:70004/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:69992/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:68673/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:10438/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:22323/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:32884/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:32406/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:31219/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:30972/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:28634/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:27825/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:2756/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:26189/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:25250/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:24966/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:24937/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:46531/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:21505/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:17474/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:16598/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:15449/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:14820/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:14277/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:13687/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:12355/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:10846/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:1034/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:34651/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:46967/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:45896/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:45389/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:45334/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:44822/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:4422/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:42702/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:41626/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:41242/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:40179/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:3973/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:38856/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:38614/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:38243/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:37873/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:36275/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:3600/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:35910/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:35727/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:35557/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:35373/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:34382/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGAGCGACGTAACGCGATAg  >  1:11114/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCTGCTTTCAAGCGTCACTGCGCGACGTAACGCGATAg  >  1:83381/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGGCTTGTCAGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:26536/1‑62 (MQ=255)
ggATGAGGCCGCTCGTCTGCCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:87604/1‑62 (MQ=255)
ggATGAGGCAGCTCGTCTGGCTTGTCTGCTTTCACGCGTCACTGCGCGACGTAACGCGATAg  >  1:88222/1‑62 (MQ=255)
                                          |                   
GGATGAGGCCGCACGCCTGGCTTGCCTGCTTTCACGCGTCACGGCGCGACGTAACGCAATAG  >  W3110S.gb/546818‑546879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A