Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 826 0.0 88706 74.7% 66263 57.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 834,802 A→T G437G (GGA→GGT)  dinG → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb834,8020AT100.0% 132.1 / NA 71G437G (GGA→GGT) dinGATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (71/0);  total (71/0)

GTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTAT  >  W3110S.gb/834797‑834858
     |                                                        
gTGGGTATTCTTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTa               >  1:70015/1‑49 (MQ=19)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCtgtg                       >  1:70430/1‑41 (MQ=19)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTTCGCACattat  >  1:10613/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:57575/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:66984/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:66597/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:6659/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:66179/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:64919/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:63699/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:62952/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:61786/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:69659/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:5703/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:56949/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:56646/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:55519/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:55172/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:5487/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:54703/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:87682/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:9235/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:9039/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:71938/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:72203/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:73450/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:74444/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:75616/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:76388/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:76819/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:77091/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:78800/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:7951/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:83653/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:83716/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:84372/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:18494/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:30696/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:30557/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:29486/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:28297/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:24931/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:24425/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:2369/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:19307/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:31618/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:17004/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:1641/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:15239/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:14816/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:14258/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:1344/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:11706/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:43582/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:51415/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:51218/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:51203/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:491/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:46751/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:45720/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:45269/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:45127/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:52817/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:43561/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:41578/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:40287/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:3905/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:37173/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:32869/1‑62 (MQ=255)
gTGGGTATTCGTGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat  >  1:32494/1‑62 (MQ=255)
gTGGGTATTCGCGTTAGCGACCAACTGGAAAGGCTGCTGTGGCGCAGTa               >  1:72530/1‑49 (MQ=19)
     |                                                        
GTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTAT  >  W3110S.gb/834797‑834858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A