Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 826 0.0 88706 74.7% 66263 57.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,277,868 G→A A443V (GCG→GTG)  narX ← sensory histidine kinase in two‑component regulatory system with NarL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,277,8680GA100.0% 163.8 / NA 93A443V (GCG→GTG) narXsensory histidine kinase in two‑component regulatory system with NarL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (93/0);  total (93/0)

GTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTT  >  W3110S.gb/1277844‑1277903
                        |                                   
gtggtgAGGAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:68374/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGtt                   >  1:42640/1‑43 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:68937/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:75582/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:74422/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:74237/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:73913/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:73738/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:71470/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:7050/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:70163/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:69169/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:69038/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:76635/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:61807/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:6180/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:61177/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:60195/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:59904/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:59886/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:59831/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:59622/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:59531/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:83516/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:9860/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:9027/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:88436/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:88390/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:85259/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:85148/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:84993/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:84784/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:84582/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:84004/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:83835/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:59487/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:83141/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:82754/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:81440/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:79938/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:79513/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:78812/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:78744/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:78026/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:77329/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:76797/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:2529/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:11721/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:34256/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:33198/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:32368/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:30245/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:2981/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:28561/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:27714/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:26495/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:26212/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:35819/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:25116/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:22022/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:20910/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:20879/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:16446/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:15847/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:14496/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:14108/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:13668/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:1206/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:37704/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:59384/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:59284/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:58189/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:56469/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:56313/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:53478/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:51535/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:50236/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:47515/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:47324/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:44639/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:44410/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:43584/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:42130/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:41681/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:40347/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:40296/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:39131/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:36286/1‑60 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACt   >  1:24240/1‑59 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACt   >  1:2177/1‑59 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACt   >  1:72447/1‑59 (MQ=255)
gtggtgAGCAATTCACGCAACTGCACCAAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:24418/1‑60 (MQ=255)
gtggagAGCAATTCACGCAACTGCACCCAGGATGCATTCAGTTCGTTACGGATCTGACtt  >  1:6772/1‑60 (MQ=255)
                        |                                   
GTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTT  >  W3110S.gb/1277844‑1277903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A