Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
826 |
0.0 |
88706 |
74.7% |
66263 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,043,856 |
T→C |
V115V (GTT→GTC) |
yodA → |
conserved metal‑binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,043,856 | 0 | T | C | 100.0%
| 56.0
/ NA
| 32 | V115V (GTT→GTC) | yodA | conserved metal‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (0/32); total (0/32) |
GCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATG > W3110S.gb/2043815‑2043875
|
gcgaCAGATATCGAAATGATTGGCATTGAGGACTGCATTGTCGAATTCCATAGAAATAATg < 1:15993/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:74771/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:54850/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:55144/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:59800/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:62404/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:69556/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:71076/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:73703/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:74423/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:5459/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:78149/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:80844/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:81183/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:85622/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:87802/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:9695/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:11119/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:50560/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:50310/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:42229/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:40914/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:36879/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:31733/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:29662/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:25951/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:25763/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:22230/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:21849/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:20989/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:17125/58‑1 (MQ=255)
gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg < 1:12414/58‑1 (MQ=255)
|
GCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATG > W3110S.gb/2043815‑2043875
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
