Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 826 0.0 88706 74.7% 66263 57.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,141,583 A→T I515I (ATA→ATT)  yegH → fused predicted membrane proteins

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,141,5830AT100.0% 265.5 / NA 139I515I (ATA→ATT) yegHfused predicted membrane proteins
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (139/0);  total (139/0)

Only 100 of 139 total aligned reads displayed.

AACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATG  >  W3110S.gb/2141538‑2141599
                                             |                
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGGAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:78704/1‑62 (MQ=255)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGcat         >  1:57153/1‑53 (MQ=37)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCGTAAGGATg  >  1:83104/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGaag  >  1:26437/1‑60 (MQ=37)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGAt   >  1:32639/1‑61 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGAt   >  1:53298/1‑61 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGAt   >  1:36556/1‑61 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGAt   >  1:34513/1‑61 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGAt   >  1:27454/1‑61 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGAt   >  1:12257/1‑61 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:6382/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:68824/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:67774/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:67664/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:86301/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:66375/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:65943/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:65754/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:64998/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:64566/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:6416/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:88315/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:62195/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:60806/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:60539/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:60194/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:58414/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:86556/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:55607/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:55479/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:53790/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:82983/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:7623/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:8234/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:80853/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:80766/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:79002/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:84429/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:78312/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:76495/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:76432/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:85019/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:85747/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:69065/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:75773/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:74506/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:7325/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:73133/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:7263/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:48498/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:72070/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:71756/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:69498/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:20686/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:31844/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:31628/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:30017/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:29122/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:28381/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:27831/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:25136/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:24069/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:22189/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:21871/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:21419/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:33754/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:19932/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:18044/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:17517/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:17441/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:1637/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:15975/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:1377/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:12236/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:10758/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:10172/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:41341/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:49530/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:1003/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:48166/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:46688/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:44287/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:42753/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:42686/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:41990/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:419/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:50063/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:39964/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:39064/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:37623/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:37286/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:35916/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:35405/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:35252/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:34337/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:34274/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:3410/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCAAAAGGATg  >  1:37763/1‑62 (MQ=37)
aaCGTTGCAGGTGGAAAGCCATAGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGAt   >  1:45840/1‑61 (MQ=37)
aaCGTTGCAGGTGGAAAGCCAACGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:88281/1‑62 (MQ=37)
                                             |                
AACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATG  >  W3110S.gb/2141538‑2141599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A