Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 826 0.0 88706 74.7% 66263 57.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,340,588 2 bp→GG coding (726‑727/1689 nt) yfaA ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,340,5880TG100.0% 139.6 / NA 75S243R (AGC→CGC) yfaAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (75/0);  total (75/0)
*W3110S.gb2,340,5890TG100.0% 139.4 / NA 75A242T (GCA→ACC) yfaAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (75/0);  total (75/0)

GCCAAAGCTTGCTTGCCAGCGTTTTTTGCCGCTCAACAAATCACCTGCGATCGCCGTGGCTT  >  W3110S.gb/2340580‑2340641
        ||                                                    
gCCAAAGCGGGTTTCCCAGCGTTTTTTACTACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:8066/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACGACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:33254/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAAt                       >  1:35935/1‑41 (MQ=16)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:65872/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:66233/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:65773/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:65284/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:63326/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:63029/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:60811/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:60307/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:59726/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:56868/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:56251/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:54437/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:52681/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:52348/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:52005/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:51546/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:475/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:79287/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:9064/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:8808/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:87401/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:87332/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:87318/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:86191/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:8619/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:82299/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:80783/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:66068/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:78339/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:71669/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:71554/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:71465/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:68940/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:68702/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:67444/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:42999/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:21144/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:27562/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:27199/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:26011/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:24158/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:24016/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:22968/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:22726/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:22682/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:28056/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:19210/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:18567/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:18137/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:17766/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:17691/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:17521/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:16633/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:16525/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:39099/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:11812/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:42153/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:41965/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:41628/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:41516/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:41083/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:40299/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:39649/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:44680/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:36136/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:36100/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:35216/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:34076/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:32710/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:31533/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACCACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:30035/1‑62 (MQ=255)
gCCAAAGCGGGTTTCCCAGCGTTTTTTACAACTCAACAAATCACTTGCGATCGCCGTAGCtt  >  1:28434/1‑62 (MQ=255)
        ||                                                    
GCCAAAGCTTGCTTGCCAGCGTTTTTTGCCGCTCAACAAATCACCTGCGATCGCCGTGGCTT  >  W3110S.gb/2340580‑2340641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A