Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
826 |
0.0 |
88706 |
74.7% |
66263 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,118,415 |
G→A |
I507I (ATC→ATT) |
yghK ← |
glycolate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,118,415 | 0 | G | A | 100.0%
| 86.6
/ NA
| 48 | I507I (ATC→ATT) | yghK | glycolate transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/48); total (0/48) |
TTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCT > W3110S.gb/3118361‑3118422
|
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:67576/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:100/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:47589/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:55314/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:57069/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:57881/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:58130/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:60026/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:60612/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:62424/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:62701/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:62885/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:46610/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:68423/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:72902/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:72908/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:74837/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:75335/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:75464/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:75948/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:77608/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:79831/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:83300/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:88085/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:29494/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:11046/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:12747/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:15280/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:19280/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:21426/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:22889/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:25002/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:29077/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:29341/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:29461/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:45633/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:30570/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:32134/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:32829/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:34940/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:35769/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:39004/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:39902/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:43812/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:44058/62‑1 (MQ=255)
ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:4406/62‑1 (MQ=255)
tctcGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:62858/61‑1 (MQ=255)
ccaTGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt < 1:28268/51‑1 (MQ=38)
|
TTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCT > W3110S.gb/3118361‑3118422
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
