Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 826 0.0 88706 74.7% 66263 57.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,153,236 G→A S213S (AGC→AGT)  yqhC ← predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,153,2360GA100.0% 135.3 / NA 75S213S (AGC→AGT) yqhCpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (75/0);  total (75/0)

ACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGGCTAATCAGACTGAA  >  W3110S.gb/3153189‑3153250
                                               |              
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGTGACTAATCAGGCTGa   >  1:78733/1‑61 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCta    >  1:77268/1‑59 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTg    >  1:54545/1‑60 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:67530/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:69717/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:66913/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:64562/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:64429/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:64115/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:58984/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:58660/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:57609/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:5405/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:53252/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:52778/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:50911/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:49400/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:47361/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:68192/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:49402/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:69726/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:72393/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:76364/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:76632/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:7738/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:77685/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:78763/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:82055/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:82278/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:84260/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:85974/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:86690/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:87900/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:9770/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:22139/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:47217/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:30622/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:28750/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:28446/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:26220/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:25652/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:25382/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:22836/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:22312/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:32127/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:20727/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:19265/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:18577/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:18206/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:17719/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:17097/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:15892/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:14711/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:36386/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:45505/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:44823/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:44052/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:43153/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:39619/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:37121/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:32438/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:35795/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:34509/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:34419/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:34077/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:33613/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGaa  >  1:33502/1‑62 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGa   >  1:74470/1‑61 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGa   >  1:54519/1‑61 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGa   >  1:7114/1‑61 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGa   >  1:5112/1‑61 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGa   >  1:64118/1‑61 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGGCTGa   >  1:12962/1‑61 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGACTAATCAGCCTGa   >  1:57294/1‑61 (MQ=255)
aCGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCAAGCGACTAATCAGGCTGaa  >  1:17834/1‑62 (MQ=255)
                                               |              
ACGCTCAGGTTTTCGGTGTATTTATTCTCAATCCGTTTCAGCACGCGGCTAATCAGACTGAA  >  W3110S.gb/3153189‑3153250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A