Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
826 |
0.0 |
88706 |
74.7% |
66263 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,463,594 |
2 bp→CG |
coding (954‑955/1353 nt) |
pmbA → |
predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,463,594 | 0 | T | C | 100.0%
| 133.4
/ NA
| 72 | I318I (ATT→ATC) | pmbA | predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (0/72); total (0/72) |
| * | W3110S.gb | 4,463,595 | 0 | A | G | 100.0%
| 140.1
/ NA
| 72 | I319V (ATT→GTC) | pmbA | predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/72); total (0/72) |
CGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCT > W3110S.gb/4463574‑4463634
||
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGTCTGCTGACCAGCt < 1:42215/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:52002/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:64166/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:61195/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:58601/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:58055/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:57059/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:55230/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:54958/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:52138/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:66196/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:51692/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:51143/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:50929/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:49138/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:44289/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:44071/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:12065/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:77909/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:88572/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:88364/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:85245/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:8354/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:82869/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:80468/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:79213/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:78525/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:44028/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:71302/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:7102/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:69035/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:68692/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:68157/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:6732/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:66300/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:19960/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:28131/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:27210/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:25844/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:25820/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:23693/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:23690/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:23340/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:22390/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:29121/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:19427/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:19277/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:17707/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:17194/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:15012/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:146/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:13814/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:13298/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:37737/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:43399/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:42894/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:42635/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:42166/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:40826/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:40725/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:40704/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:39661/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:43759/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:33752/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:33004/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:32387/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:30452/61‑1 (MQ=255)
cgcACCGAGCGCCGCGATATCGGCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:67018/61‑1 (MQ=255)
gcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:36496/60‑1 (MQ=255)
gcACCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:32072/60‑1 (MQ=255)
aCCGAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:5192/58‑1 (MQ=255)
gAGCGCCGCGATATCGTCAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCt < 1:55845/55‑1 (MQ=255)
||
CGCACCGAGCGTCGCGATATTATTAAAGATGGCATCCTGACTCAGTGGCTGCTGACCAGCT > W3110S.gb/4463574‑4463634
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
