Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
332,960 |
T→A |
Q233L (CAG→CTG) |
yahB ← |
predicted DNA‑bindng transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 332,960 | 0 | T | A | 100.0%
| 19.5
/ NA
| 8 | Q233L (CAG→CTG) | yahB | predicted DNA‑bindng transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (8/0); total (8/0) |
CTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATT > W3110S.gb/332905‑332966
|
cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt > 1:1110872/1‑62 (MQ=255)
cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt > 1:1989128/1‑62 (MQ=255)
cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt > 1:2289906/1‑62 (MQ=255)
cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt > 1:2800865/1‑62 (MQ=255)
cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt > 1:2801845/1‑62 (MQ=255)
cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt > 1:302169/1‑62 (MQ=255)
cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt > 1:719359/1‑62 (MQ=255)
cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt > 1:857429/1‑62 (MQ=255)
|
CTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATT > W3110S.gb/332905‑332966
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
