Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
429,762 |
A→T |
intergenic (+62/‑67) |
secF → / → yajD |
SecYEG protein translocase auxillary subunit/conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 429,762 | 0 | A | T | 81.6%
| 111.7
/ 19.9
| 49 | intergenic (+62/‑67) | secF/yajD | SecYEG protein translocase auxillary subunit/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (7/2); new base T (0/40); total (7/42) |
| Fisher's exact test for biased strand distribution p-value = 4.19e-07 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 |
TCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTC > W3110S.gb/429707‑429812
|
tCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTg > 1:2348104/1‑61 (MQ=255)
tCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTg > 1:527544/1‑61 (MQ=255)
tCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTg > 1:525627/1‑61 (MQ=255)
tCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTg > 1:2134999/1‑61 (MQ=255)
tCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTg > 1:1731472/1‑61 (MQ=255)
tCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTg > 1:2072952/1‑61 (MQ=255)
tCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTg > 1:1746963/1‑61 (MQ=255)
aTTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCt < 1:133079/62‑1 (MQ=255)
aTTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCt < 1:1635191/62‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:387628/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2321770/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2429130/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2529183/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2607741/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2674720/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2774684/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2781401/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2787180/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2804222/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2244557/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:444440/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:481940/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:523433/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:525994/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:681927/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:914108/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2286030/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:111645/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1188332/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1212526/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1331440/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1514773/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1618053/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1657256/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1736233/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1748739/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:177620/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1794408/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1808801/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1883844/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2006540/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2035986/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2107522/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2143410/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1094580/61‑1 (MQ=255)
tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2319410/61‑1 (MQ=255)
gATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2911600/60‑1 (MQ=255)
gATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:496643/60‑1 (MQ=255)
gATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:824696/60‑1 (MQ=255)
|
TCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTC > W3110S.gb/429707‑429812
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
