Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
486,836 |
G→A |
A359A (GCG→GCA) |
kefA → |
fused mechanosensitive channel proteins |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 486,836 | 0 | G | A | 75.4%
| 109.7
/ 31.6
| 65 | A359A (GCG→GCA) | kefA | fused mechanosensitive channel proteins |
| Reads supporting (aligned to +/- strand): ref base G (0/16); new base A (49/0); total (49/16) |
| Fisher's exact test for biased strand distribution p-value = 1.54e-15 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTGCTGTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAA > W3110S.gb/486786‑486893
|
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGa > 1:1855020/1‑56 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2884081/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2089688/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2095060/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2161700/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2175270/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2188077/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2190367/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2205327/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2278927/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2385515/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2425898/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2601543/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2724623/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2759978/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1184895/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:364425/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:386113/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:392385/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:420020/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:503862/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:508112/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:542518/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:587652/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:665059/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:845169/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:904239/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:911840/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:948094/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1937820/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1798240/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1227499/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1871057/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1882546/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1894827/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1910216/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1927762/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1217898/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1946007/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:195024/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1213648/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1963184/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1965338/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1188783/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1997249/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTg > 1:976194/1‑60 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTg > 1:1867712/1‑60 (MQ=255)
ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTAGGCAGATGAACTgg > 1:540106/1‑61 (MQ=255)
ctgctgTTGTCACGTATCCTCTACCAGCAACAACAAACACTCCCCTCGGCAGATGAAc > 1:20110/1‑58 (MQ=255)
gtCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:100084/62‑1 (MQ=255)
gtCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:21047/62‑1 (MQ=255)
gtCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:1564645/62‑1 (MQ=255)
gtCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:1556384/62‑1 (MQ=255)
gtCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:2187547/62‑1 (MQ=255)
gtCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:69363/62‑1 (MQ=255)
gtCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:2787516/62‑1 (MQ=255)
tctcGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:1261116/61‑1 (MQ=255)
tctcGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:2742204/61‑1 (MQ=255)
cTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGttt < 1:1496773/61‑1 (MQ=255)
cTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGttt < 1:806687/61‑1 (MQ=255)
cTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGttt < 1:1552285/61‑1 (MQ=255)
cTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGttt < 1:1203855/61‑1 (MQ=255)
cTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGttt < 1:1661931/61‑1 (MQ=255)
cTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGttt < 1:31735/61‑1 (MQ=255)
cTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGttt < 1:1821289/61‑1 (MQ=255)
cggcggATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGaa < 1:222804/62‑1 (MQ=255)
|
CTGCTGTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAA > W3110S.gb/486786‑486893
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
