Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
589,308 |
G→T |
L290L (CTC→CTA) |
nfrA ← |
bacteriophage N4 receptor, outer membrane subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 589,308 | 0 | G | T | 100.0%
| 20.5
/ NA
| 8 | L290L (CTC→CTA) | nfrA | bacteriophage N4 receptor, outer membrane subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (0/8); total (0/8) |
TTAGCGCTGTATTTAGATAACAGATAGAGCCAACTTTTCTCTTGTGCGTCCGTGGTAAATAG > W3110S.gb/589282‑589343
|
ttAGCGCTGTATTTAGATAACAGATATAGCCAACTTTTCTCTTGTGCGTCCGTGGTAAATAg < 1:1055536/62‑1 (MQ=255)
ttAGCGCTGTATTTAGATAACAGATATAGCCAACTTTTCTCTTGTGCGTCCGTGGTAAATAg < 1:1953144/62‑1 (MQ=255)
ttAGCGCTGTATTTAGATAACAGATATAGCCAACTTTTCTCTTGTGCGTCCGTGGTAAATAg < 1:2155510/62‑1 (MQ=255)
ttAGCGCTGTATTTAGATAACAGATATAGCCAACTTTTCTCTTGTGCGTCCGTGGTAAATAg < 1:2237006/62‑1 (MQ=255)
ttAGCGCTGTATTTAGATAACAGATATAGCCAACTTTTCTCTTGTGCGTCCGTGGTAAATAg < 1:478022/62‑1 (MQ=255)
ttAGCGCTGTATTTAGATAACAGATATAGCCAACTTTTCTCTTGTGCGTCCGTGGTAAATAg < 1:516524/62‑1 (MQ=255)
ttAGCGCTGTATTTAGATAACAGATATAGCCAACTTTTCTCTTGTGCGTCCGTGGTAAATAg < 1:938999/62‑1 (MQ=255)
ttAGCGCTGTATTTAGATAACAGATATAGCCAACTTTTCTCTTGTGCGTCCGTGGTAAATAg < 1:98870/62‑1 (MQ=255)
|
TTAGCGCTGTATTTAGATAACAGATAGAGCCAACTTTTCTCTTGTGCGTCCGTGGTAAATAG > W3110S.gb/589282‑589343
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
