Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
635,223 |
T→C |
K190K (AAA→AAG) |
ybdN ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 635,223 | 0 | T | C | 100.0%
| 67.5
/ NA
| 21 | K190K (AAA→AAG) | ybdN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (0/21); total (0/21) |
AGTCCAGGGTTTATCGTCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGT > W3110S.gb/635214‑635275
|
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:2536534/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:836053/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:651185/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:618334/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:609675/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:525781/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:343244/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:2869465/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:2849119/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:2819982/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:2602856/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:2531425/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:244807/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:1937299/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:1823317/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:1571876/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:1515885/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:1393473/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:1374715/62‑1 (MQ=255)
aGTCCAGGGCTTATCATCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:1310366/62‑1 (MQ=255)
ccAGGGCTTATCATCTGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGt < 1:1182069/59‑1 (MQ=255)
|
AGTCCAGGGTTTATCGTCGGCAAAACGTTGTTTATTTAAACTGGCGATGGCGACAAAACGGT > W3110S.gb/635214‑635275
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
